Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1436 and Psest_1437 are separated by 135 nucleotides Psest_1437 and Psest_1438 are separated by 56 nucleotides Psest_1438 and Psest_1439 are separated by 4 nucleotides Psest_1439 and Psest_1440 are separated by 261 nucleotides
Psest_1436: Psest_1436 - Uncharacterized protein conserved in bacteria, at 1,547,293 to 1,547,745
_1436
Psest_1437: Psest_1437 - Uncharacterized protein conserved in archaea, at 1,547,881 to 1,548,399
_1437
Psest_1438: Psest_1438 - Transcriptional regulator, at 1,548,456 to 1,549,415
_1438
Psest_1439: Psest_1439 - Transcriptional regulator, at 1,549,420 to 1,550,091
_1439
Psest_1440: Psest_1440 - Nucleoside-binding outer membrane protein, at 1,550,353 to 1,551,168
_1440
Position (kb)
1548
1549
1550 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1547.581 kb on + strand, within Psest_1436 at 1547.581 kb on + strand, within Psest_1436 at 1547.582 kb on - strand, within Psest_1436 at 1547.627 kb on + strand, within Psest_1436 at 1547.627 kb on + strand, within Psest_1436 at 1547.628 kb on - strand, within Psest_1436 at 1547.628 kb on - strand, within Psest_1436 at 1547.628 kb on - strand, within Psest_1436 at 1547.678 kb on + strand, within Psest_1436 at 1547.745 kb on + strand at 1547.746 kb on - strand at 1547.746 kb on - strand at 1548.209 kb on + strand, within Psest_1437 at 1548.209 kb on + strand, within Psest_1437 at 1548.210 kb on - strand, within Psest_1437 at 1548.361 kb on + strand at 1548.361 kb on + strand at 1548.361 kb on + strand at 1548.361 kb on + strand at 1548.362 kb on - strand at 1548.362 kb on - strand at 1548.362 kb on - strand at 1548.362 kb on - strand at 1548.432 kb on + strand at 1548.432 kb on + strand at 1548.433 kb on - strand at 1548.433 kb on - strand at 1548.433 kb on - strand at 1548.565 kb on + strand, within Psest_1438 at 1548.566 kb on - strand, within Psest_1438 at 1548.566 kb on - strand, within Psest_1438 at 1548.583 kb on + strand, within Psest_1438 at 1548.583 kb on + strand, within Psest_1438 at 1548.583 kb on + strand, within Psest_1438 at 1548.583 kb on + strand, within Psest_1438 at 1548.584 kb on - strand, within Psest_1438 at 1548.584 kb on - strand, within Psest_1438 at 1548.584 kb on - strand, within Psest_1438 at 1548.641 kb on + strand, within Psest_1438 at 1549.144 kb on - strand, within Psest_1438 at 1549.144 kb on - strand, within Psest_1438 at 1549.376 kb on - strand at 1549.376 kb on - strand at 1549.376 kb on - strand at 1549.785 kb on - strand, within Psest_1439 at 1549.785 kb on - strand, within Psest_1439 at 1549.877 kb on + strand, within Psest_1439 at 1549.877 kb on + strand, within Psest_1439 at 1549.877 kb on + strand, within Psest_1439 at 1549.877 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.901 kb on + strand, within Psest_1439 at 1549.902 kb on - strand, within Psest_1439 at 1549.902 kb on - strand, within Psest_1439 at 1549.902 kb on - strand, within Psest_1439 at 1549.902 kb on - strand, within Psest_1439 at 1549.902 kb on - strand, within Psest_1439 at 1549.902 kb on - strand, within Psest_1439 at 1549.904 kb on + strand, within Psest_1439 at 1549.905 kb on - strand, within Psest_1439 at 1549.905 kb on - strand, within Psest_1439 at 1550.060 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 1,547,581 + Psest_1436 0.64 +4.0 1,547,581 + Psest_1436 0.64 -0.3 1,547,582 - Psest_1436 0.64 +0.3 1,547,627 + Psest_1436 0.74 -1.5 1,547,627 + Psest_1436 0.74 +1.1 1,547,628 - Psest_1436 0.74 +0.6 1,547,628 - Psest_1436 0.74 +1.9 1,547,628 - Psest_1436 0.74 +1.7 1,547,678 + Psest_1436 0.85 +2.0 1,547,745 + +1.0 1,547,746 - -1.6 1,547,746 - +2.8 1,548,209 + Psest_1437 0.63 -0.4 1,548,209 + Psest_1437 0.63 -0.2 1,548,210 - Psest_1437 0.63 -1.4 1,548,361 + -2.2 1,548,361 + +3.1 1,548,361 + -1.7 1,548,361 + +2.8 1,548,362 - -1.9 1,548,362 - +1.7 1,548,362 - +2.9 1,548,362 - -1.8 1,548,432 + +2.2 1,548,432 + -2.5 1,548,433 - +3.5 1,548,433 - +1.8 1,548,433 - +0.6 1,548,565 + Psest_1438 0.11 -1.1 1,548,566 - Psest_1438 0.11 +0.4 1,548,566 - Psest_1438 0.11 -1.8 1,548,583 + Psest_1438 0.13 +0.4 1,548,583 + Psest_1438 0.13 -1.3 1,548,583 + Psest_1438 0.13 +1.7 1,548,583 + Psest_1438 0.13 +0.4 1,548,584 - Psest_1438 0.13 -1.5 1,548,584 - Psest_1438 0.13 +0.8 1,548,584 - Psest_1438 0.13 -0.5 1,548,641 + Psest_1438 0.19 -0.6 1,549,144 - Psest_1438 0.72 -2.2 1,549,144 - Psest_1438 0.72 -0.7 1,549,376 - -1.0 1,549,376 - -1.6 1,549,376 - -1.5 1,549,785 - Psest_1439 0.54 +0.2 1,549,785 - Psest_1439 0.54 +3.2 1,549,877 + Psest_1439 0.68 -1.5 1,549,877 + Psest_1439 0.68 -2.0 1,549,877 + Psest_1439 0.68 +1.2 1,549,877 + Psest_1439 0.68 +1.7 1,549,901 + Psest_1439 0.72 +3.2 1,549,901 + Psest_1439 0.72 -0.5 1,549,901 + Psest_1439 0.72 +1.7 1,549,901 + Psest_1439 0.72 -0.6 1,549,901 + Psest_1439 0.72 +3.0 1,549,901 + Psest_1439 0.72 -0.4 1,549,901 + Psest_1439 0.72 +4.0 1,549,901 + Psest_1439 0.72 -0.5 1,549,901 + Psest_1439 0.72 -0.2 1,549,901 + Psest_1439 0.72 +2.2 1,549,901 + Psest_1439 0.72 +1.1 1,549,901 + Psest_1439 0.72 +0.5 1,549,901 + Psest_1439 0.72 -0.8 1,549,901 + Psest_1439 0.72 +0.2 1,549,901 + Psest_1439 0.72 +1.8 1,549,902 - Psest_1439 0.72 -2.1 1,549,902 - Psest_1439 0.72 +0.6 1,549,902 - Psest_1439 0.72 +2.8 1,549,902 - Psest_1439 0.72 -1.1 1,549,902 - Psest_1439 0.72 -0.6 1,549,902 - Psest_1439 0.72 +1.5 1,549,904 + Psest_1439 0.72 -0.2 1,549,905 - Psest_1439 0.72 -0.4 1,549,905 - Psest_1439 0.72 -1.2 1,550,060 + +0.3
Or see this region's nucleotide sequence