Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1159 and Psest_1160 are separated by 169 nucleotides Psest_1160 and Psest_1161 are separated by 1 nucleotides Psest_1161 and Psest_1162 are separated by 14 nucleotides Psest_1162 and Psest_1163 are separated by 125 nucleotides
Psest_1159: Psest_1159 - Uncharacterized iron-regulated protein, at 1,259,680 to 1,261,014
_1159
Psest_1160: Psest_1160 - Uncharacterized conserved protein, at 1,261,184 to 1,261,756
_1160
Psest_1161: Psest_1161 - E3 Ubiquitin ligase., at 1,261,758 to 1,262,651
_1161
Psest_1162: Psest_1162 - Spermidine synthase, at 1,262,666 to 1,263,349
_1162
Psest_1163: Psest_1163 - diguanylate cyclase (GGDEF) domain, at 1,263,475 to 1,265,535
_1163
Position (kb) 1261
1262
1263 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 1261.010 kb on - strand at 1261.010 kb on - strand at 1261.010 kb on - strand at 1261.235 kb on + strand at 1261.235 kb on + strand at 1261.236 kb on - strand at 1261.250 kb on + strand, within Psest_1160 at 1261.251 kb on - strand, within Psest_1160 at 1261.251 kb on - strand, within Psest_1160 at 1261.364 kb on + strand, within Psest_1160 at 1261.607 kb on + strand, within Psest_1160 at 1261.607 kb on + strand, within Psest_1160 at 1261.608 kb on - strand, within Psest_1160 at 1261.899 kb on + strand, within Psest_1161 at 1261.900 kb on - strand, within Psest_1161 at 1261.900 kb on - strand, within Psest_1161 at 1262.260 kb on - strand, within Psest_1161 at 1262.636 kb on + strand at 1262.636 kb on + strand at 1263.078 kb on - strand, within Psest_1162 at 1263.078 kb on - strand, within Psest_1162 at 1263.156 kb on - strand, within Psest_1162 at 1263.336 kb on - strand at 1263.336 kb on - strand at 1263.336 kb on - strand at 1263.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 1,261,010 - -3.2 1,261,010 - -2.5 1,261,010 - -0.5 1,261,235 + -2.5 1,261,235 + +1.3 1,261,236 - +2.9 1,261,250 + Psest_1160 0.12 +0.4 1,261,251 - Psest_1160 0.12 -0.9 1,261,251 - Psest_1160 0.12 -0.1 1,261,364 + Psest_1160 0.31 -0.9 1,261,607 + Psest_1160 0.74 +3.5 1,261,607 + Psest_1160 0.74 -0.9 1,261,608 - Psest_1160 0.74 -1.2 1,261,899 + Psest_1161 0.16 +3.9 1,261,900 - Psest_1161 0.16 -2.6 1,261,900 - Psest_1161 0.16 +2.0 1,262,260 - Psest_1161 0.56 -1.5 1,262,636 + +1.7 1,262,636 + -0.9 1,263,078 - Psest_1162 0.60 -2.5 1,263,078 - Psest_1162 0.60 -1.0 1,263,156 - Psest_1162 0.72 -2.3 1,263,336 - -1.6 1,263,336 - -1.6 1,263,336 - -0.1 1,263,448 - -0.4
Or see this region's nucleotide sequence