Experiment: RCH2 grown on a colony of Serratia
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1132 and Psest_1133 are separated by 172 nucleotides Psest_1133 and Psest_1134 are separated by 119 nucleotides Psest_1134 and Psest_1135 are separated by 212 nucleotides
Psest_1132: Psest_1132 - mutator mutT protein, at 1,228,882 to 1,229,820
_1132
Psest_1133: Psest_1133 - peroxiredoxin, at 1,229,993 to 1,230,556
_1133
Psest_1134: Psest_1134 - alkyl hydroperoxide reductase, F subunit, at 1,230,676 to 1,232,238
_1134
Psest_1135: Psest_1135 - Protein of unknown function (DUF3182)., at 1,232,451 to 1,233,596
_1135
Position (kb)
1230
1231
1232
1233 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1229.705 kb on + strand, within Psest_1132 at 1229.726 kb on - strand, within Psest_1132 at 1229.836 kb on + strand at 1229.836 kb on + strand at 1229.836 kb on + strand at 1229.836 kb on + strand at 1229.837 kb on - strand at 1229.837 kb on - strand at 1229.837 kb on - strand at 1229.837 kb on - strand at 1229.861 kb on + strand at 1229.861 kb on + strand at 1229.875 kb on + strand at 1229.945 kb on + strand at 1229.945 kb on + strand at 1229.950 kb on + strand at 1230.554 kb on + strand at 1230.554 kb on + strand at 1230.554 kb on + strand at 1230.554 kb on + strand at 1230.555 kb on - strand at 1230.643 kb on - strand at 1231.195 kb on + strand, within Psest_1134 at 1231.360 kb on + strand, within Psest_1134 at 1231.360 kb on + strand, within Psest_1134 at 1231.360 kb on + strand, within Psest_1134 at 1231.954 kb on + strand, within Psest_1134 at 1231.979 kb on + strand, within Psest_1134 at 1231.979 kb on + strand, within Psest_1134 at 1231.979 kb on + strand, within Psest_1134 at 1231.979 kb on + strand, within Psest_1134 at 1231.979 kb on + strand, within Psest_1134 at 1231.980 kb on - strand, within Psest_1134 at 1231.980 kb on - strand, within Psest_1134 at 1231.980 kb on - strand, within Psest_1134 at 1232.293 kb on + strand at 1232.293 kb on + strand at 1232.434 kb on + strand at 1232.434 kb on + strand at 1232.434 kb on + strand at 1232.434 kb on + strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.435 kb on - strand at 1232.649 kb on + strand, within Psest_1135 at 1232.671 kb on + strand, within Psest_1135 at 1232.671 kb on + strand, within Psest_1135 at 1232.671 kb on + strand, within Psest_1135 at 1232.671 kb on + strand, within Psest_1135 at 1232.672 kb on - strand, within Psest_1135 at 1232.672 kb on - strand, within Psest_1135 at 1233.040 kb on + strand, within Psest_1135 at 1233.040 kb on + strand, within Psest_1135 at 1233.040 kb on + strand, within Psest_1135 at 1233.041 kb on - strand, within Psest_1135 at 1233.117 kb on + strand, within Psest_1135 at 1233.117 kb on + strand, within Psest_1135 at 1233.117 kb on + strand, within Psest_1135 at 1233.117 kb on + strand, within Psest_1135 at 1233.117 kb on + strand, within Psest_1135 at 1233.117 kb on + strand, within Psest_1135 at 1233.117 kb on + strand, within Psest_1135 at 1233.117 kb on + strand, within Psest_1135 at 1233.118 kb on - strand, within Psest_1135 at 1233.118 kb on - strand, within Psest_1135 at 1233.118 kb on - strand, within Psest_1135 at 1233.146 kb on + strand, within Psest_1135 at 1233.165 kb on + strand, within Psest_1135
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of Serratia remove 1,229,705 + Psest_1132 0.88 -0.7 1,229,726 - Psest_1132 0.90 +0.8 1,229,836 + +3.3 1,229,836 + -1.3 1,229,836 + -1.5 1,229,836 + +2.4 1,229,837 - -1.8 1,229,837 - -0.8 1,229,837 - +1.6 1,229,837 - +0.5 1,229,861 + -0.7 1,229,861 + +0.8 1,229,875 + +0.7 1,229,945 + -2.0 1,229,945 + +0.5 1,229,950 + -1.2 1,230,554 + -1.3 1,230,554 + -2.2 1,230,554 + +1.9 1,230,554 + +0.7 1,230,555 - -0.3 1,230,643 - +0.8 1,231,195 + Psest_1134 0.33 +0.7 1,231,360 + Psest_1134 0.44 -0.3 1,231,360 + Psest_1134 0.44 +1.6 1,231,360 + Psest_1134 0.44 +0.7 1,231,954 + Psest_1134 0.82 -0.9 1,231,979 + Psest_1134 0.83 +2.1 1,231,979 + Psest_1134 0.83 -0.9 1,231,979 + Psest_1134 0.83 +3.5 1,231,979 + Psest_1134 0.83 +1.3 1,231,979 + Psest_1134 0.83 -0.1 1,231,980 - Psest_1134 0.83 -1.0 1,231,980 - Psest_1134 0.83 +0.6 1,231,980 - Psest_1134 0.83 +4.2 1,232,293 + -2.2 1,232,293 + -1.5 1,232,434 + -0.1 1,232,434 + -0.7 1,232,434 + +1.3 1,232,434 + -0.2 1,232,435 - -0.3 1,232,435 - -1.7 1,232,435 - -0.6 1,232,435 - -0.3 1,232,435 - -0.5 1,232,435 - -0.3 1,232,435 - +0.7 1,232,435 - -1.6 1,232,435 - -2.5 1,232,435 - -0.3 1,232,435 - -1.3 1,232,435 - -0.2 1,232,435 - -1.7 1,232,435 - +0.3 1,232,435 - -0.4 1,232,435 - +2.8 1,232,649 + Psest_1135 0.17 -0.8 1,232,671 + Psest_1135 0.19 +0.3 1,232,671 + Psest_1135 0.19 -0.0 1,232,671 + Psest_1135 0.19 -0.7 1,232,671 + Psest_1135 0.19 -1.0 1,232,672 - Psest_1135 0.19 +1.6 1,232,672 - Psest_1135 0.19 -0.1 1,233,040 + Psest_1135 0.51 +0.3 1,233,040 + Psest_1135 0.51 +2.7 1,233,040 + Psest_1135 0.51 +1.8 1,233,041 - Psest_1135 0.51 +1.5 1,233,117 + Psest_1135 0.58 -0.5 1,233,117 + Psest_1135 0.58 -0.1 1,233,117 + Psest_1135 0.58 -0.7 1,233,117 + Psest_1135 0.58 -1.5 1,233,117 + Psest_1135 0.58 -0.6 1,233,117 + Psest_1135 0.58 +1.7 1,233,117 + Psest_1135 0.58 -1.7 1,233,117 + Psest_1135 0.58 +2.9 1,233,118 - Psest_1135 0.58 +1.3 1,233,118 - Psest_1135 0.58 +2.1 1,233,118 - Psest_1135 0.58 -1.8 1,233,146 + Psest_1135 0.61 +1.4 1,233,165 + Psest_1135 0.62 -2.2
Or see this region's nucleotide sequence