Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_1004

Experiment: RCH2 grown on a colony of ecoli

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_1002 and Psest_1003 are separated by 89 nucleotidesPsest_1003 and Psest_1004 are separated by 191 nucleotidesPsest_1004 and Psest_1005 overlap by 1 nucleotidesPsest_1005 and Psest_1006 are separated by 263 nucleotides Psest_1002: Psest_1002 - glutamyl-queuosine tRNA(Asp) synthetase, at 1,087,302 to 1,088,207 _1002 Psest_1003: Psest_1003 - RNA polymerase-binding protein DksA, at 1,088,297 to 1,088,743 _1003 Psest_1004: Psest_1004 - Aspartate/tyrosine/aromatic aminotransferase, at 1,088,935 to 1,090,107 _1004 Psest_1005: Psest_1005 - sugar fermentation stimulation protein, at 1,090,107 to 1,090,814 _1005 Psest_1006: Psest_1006 - dTDP-4-dehydrorhamnose reductase, at 1,091,078 to 1,091,998 _1006 Position (kb) 1088 1089 1090 1091Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4 5at 1087.990 kb on + strand, within Psest_1002at 1087.991 kb on - strand, within Psest_1002at 1087.991 kb on - strand, within Psest_1002at 1088.101 kb on + strand, within Psest_1002at 1088.102 kb on - strand, within Psest_1002at 1088.102 kb on - strand, within Psest_1002at 1088.686 kb on - strand, within Psest_1003at 1088.686 kb on - strand, within Psest_1003at 1088.901 kb on - strandat 1088.947 kb on + strandat 1088.947 kb on + strandat 1088.947 kb on + strandat 1088.947 kb on + strandat 1088.947 kb on + strandat 1088.947 kb on + strandat 1088.947 kb on + strandat 1088.947 kb on + strandat 1088.948 kb on - strandat 1088.948 kb on - strandat 1088.948 kb on - strandat 1088.948 kb on - strandat 1089.128 kb on - strand, within Psest_1004at 1089.130 kb on - strand, within Psest_1004at 1089.197 kb on + strand, within Psest_1004at 1089.197 kb on + strand, within Psest_1004at 1089.197 kb on + strand, within Psest_1004at 1089.198 kb on - strand, within Psest_1004at 1089.198 kb on - strand, within Psest_1004at 1089.198 kb on - strand, within Psest_1004at 1089.198 kb on - strand, within Psest_1004at 1089.202 kb on - strand, within Psest_1004at 1089.202 kb on - strand, within Psest_1004at 1089.553 kb on + strand, within Psest_1004at 1089.553 kb on + strand, within Psest_1004at 1089.553 kb on + strand, within Psest_1004at 1089.553 kb on + strand, within Psest_1004at 1089.554 kb on - strand, within Psest_1004at 1089.554 kb on - strand, within Psest_1004at 1089.554 kb on - strand, within Psest_1004at 1089.554 kb on - strand, within Psest_1004at 1089.569 kb on - strand, within Psest_1004at 1089.600 kb on - strand, within Psest_1004at 1089.637 kb on + strand, within Psest_1004at 1089.637 kb on + strand, within Psest_1004at 1089.637 kb on + strand, within Psest_1004at 1089.638 kb on - strand, within Psest_1004at 1089.638 kb on - strand, within Psest_1004at 1089.638 kb on - strand, within Psest_1004at 1089.724 kb on + strand, within Psest_1004at 1089.725 kb on - strand, within Psest_1004at 1089.725 kb on - strand, within Psest_1004at 1089.867 kb on + strand, within Psest_1004at 1089.896 kb on - strand, within Psest_1004at 1089.896 kb on - strand, within Psest_1004at 1089.896 kb on - strand, within Psest_1004at 1089.902 kb on - strand, within Psest_1004at 1089.902 kb on - strand, within Psest_1004at 1090.034 kb on - strandat 1090.034 kb on - strandat 1090.152 kb on + strandat 1090.443 kb on + strand, within Psest_1005at 1090.444 kb on - strand, within Psest_1005at 1090.444 kb on - strand, within Psest_1005at 1090.444 kb on - strand, within Psest_1005at 1090.444 kb on - strand, within Psest_1005at 1090.444 kb on - strand, within Psest_1005at 1090.642 kb on - strand, within Psest_1005at 1090.642 kb on - strand, within Psest_1005at 1090.699 kb on + strand, within Psest_1005at 1090.699 kb on + strand, within Psest_1005at 1090.700 kb on - strand, within Psest_1005at 1090.700 kb on - strand, within Psest_1005at 1090.701 kb on + strand, within Psest_1005at 1090.943 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli
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1,087,990 + Psest_1002 0.76 +0.4
1,087,991 - Psest_1002 0.76 -1.8
1,087,991 - Psest_1002 0.76 +1.0
1,088,101 + Psest_1002 0.88 -0.8
1,088,102 - Psest_1002 0.88 +0.3
1,088,102 - Psest_1002 0.88 -2.1
1,088,686 - Psest_1003 0.87 -1.4
1,088,686 - Psest_1003 0.87 -1.6
1,088,901 - -0.4
1,088,947 + +0.6
1,088,947 + -1.9
1,088,947 + +5.1
1,088,947 + +1.2
1,088,947 + -0.9
1,088,947 + -2.6
1,088,947 + +0.0
1,088,947 + +1.7
1,088,948 - -1.5
1,088,948 - -2.2
1,088,948 - +4.7
1,088,948 - -2.0
1,089,128 - Psest_1004 0.16 -2.6
1,089,130 - Psest_1004 0.17 -0.2
1,089,197 + Psest_1004 0.22 -1.6
1,089,197 + Psest_1004 0.22 -1.5
1,089,197 + Psest_1004 0.22 -0.2
1,089,198 - Psest_1004 0.22 -0.4
1,089,198 - Psest_1004 0.22 -1.4
1,089,198 - Psest_1004 0.22 +1.0
1,089,198 - Psest_1004 0.22 +0.7
1,089,202 - Psest_1004 0.23 -2.7
1,089,202 - Psest_1004 0.23 -2.5
1,089,553 + Psest_1004 0.53 +1.4
1,089,553 + Psest_1004 0.53 -3.0
1,089,553 + Psest_1004 0.53 +0.1
1,089,553 + Psest_1004 0.53 -0.2
1,089,554 - Psest_1004 0.53 -2.9
1,089,554 - Psest_1004 0.53 -2.0
1,089,554 - Psest_1004 0.53 -0.8
1,089,554 - Psest_1004 0.53 -1.2
1,089,569 - Psest_1004 0.54 +2.2
1,089,600 - Psest_1004 0.57 -0.9
1,089,637 + Psest_1004 0.60 +0.9
1,089,637 + Psest_1004 0.60 -3.0
1,089,637 + Psest_1004 0.60 -0.7
1,089,638 - Psest_1004 0.60 -1.8
1,089,638 - Psest_1004 0.60 +0.2
1,089,638 - Psest_1004 0.60 +1.5
1,089,724 + Psest_1004 0.67 +0.8
1,089,725 - Psest_1004 0.67 +0.8
1,089,725 - Psest_1004 0.67 -1.8
1,089,867 + Psest_1004 0.79 +1.2
1,089,896 - Psest_1004 0.82 +1.5
1,089,896 - Psest_1004 0.82 -0.0
1,089,896 - Psest_1004 0.82 -3.1
1,089,902 - Psest_1004 0.82 +0.4
1,089,902 - Psest_1004 0.82 -0.6
1,090,034 - +1.7
1,090,034 - -1.9
1,090,152 + +2.8
1,090,443 + Psest_1005 0.47 -0.6
1,090,444 - Psest_1005 0.48 -3.1
1,090,444 - Psest_1005 0.48 +2.0
1,090,444 - Psest_1005 0.48 -3.5
1,090,444 - Psest_1005 0.48 +1.2
1,090,444 - Psest_1005 0.48 +2.4
1,090,642 - Psest_1005 0.76 -0.4
1,090,642 - Psest_1005 0.76 -1.2
1,090,699 + Psest_1005 0.84 +1.3
1,090,699 + Psest_1005 0.84 +2.8
1,090,700 - Psest_1005 0.84 +0.1
1,090,700 - Psest_1005 0.84 -1.3
1,090,701 + Psest_1005 0.84 -3.2
1,090,943 - +0.8

Or see this region's nucleotide sequence