Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0423 and Psest_0424 overlap by 8 nucleotides
Psest_0423: Psest_0423 - hypothetical protein, at 459,051 to 461,111
_0423
Psest_0424: Psest_0424 - Phage integrase family., at 461,104 to 462,975
_0424
Position (kb) 461
462
463 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 460.128 kb on + strand, within Psest_0423 at 460.131 kb on + strand, within Psest_0423 at 460.132 kb on - strand, within Psest_0423 at 460.149 kb on - strand, within Psest_0423 at 460.189 kb on + strand, within Psest_0423 at 460.320 kb on - strand, within Psest_0423 at 460.349 kb on + strand, within Psest_0423 at 460.419 kb on - strand, within Psest_0423 at 460.539 kb on + strand, within Psest_0423 at 460.540 kb on - strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.543 kb on + strand, within Psest_0423 at 460.563 kb on + strand, within Psest_0423 at 460.563 kb on + strand, within Psest_0423 at 460.564 kb on - strand, within Psest_0423 at 460.740 kb on - strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.822 kb on + strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.823 kb on - strand, within Psest_0423 at 460.832 kb on + strand, within Psest_0423 at 460.871 kb on - strand, within Psest_0423 at 460.941 kb on - strand at 460.998 kb on + strand at 461.448 kb on + strand, within Psest_0424 at 461.598 kb on + strand, within Psest_0424 at 461.613 kb on + strand, within Psest_0424 at 461.664 kb on + strand, within Psest_0424 at 461.679 kb on + strand, within Psest_0424 at 461.744 kb on + strand, within Psest_0424 at 461.823 kb on - strand, within Psest_0424 at 461.869 kb on + strand, within Psest_0424 at 461.870 kb on - strand, within Psest_0424 at 461.919 kb on + strand, within Psest_0424 at 461.963 kb on - strand, within Psest_0424 at 461.987 kb on + strand, within Psest_0424 at 462.075 kb on + strand, within Psest_0424 at 462.075 kb on + strand, within Psest_0424 at 462.261 kb on + strand, within Psest_0424 at 462.262 kb on - strand, within Psest_0424 at 462.432 kb on + strand, within Psest_0424 at 462.600 kb on + strand, within Psest_0424 at 462.636 kb on + strand, within Psest_0424 at 462.636 kb on + strand, within Psest_0424 at 462.648 kb on + strand, within Psest_0424 at 462.648 kb on + strand, within Psest_0424 at 462.669 kb on + strand, within Psest_0424 at 462.692 kb on + strand, within Psest_0424 at 462.692 kb on + strand, within Psest_0424 at 462.735 kb on + strand, within Psest_0424 at 462.825 kb on + strand at 462.850 kb on - strand at 463.103 kb on + strand at 463.103 kb on + strand at 463.195 kb on + strand at 463.340 kb on + strand at 463.340 kb on + strand at 463.361 kb on + strand at 463.361 kb on + strand at 463.363 kb on + strand at 463.453 kb on + strand at 463.497 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.570 kb on + strand at 463.746 kb on + strand at 463.796 kb on + strand at 463.797 kb on - strand at 463.836 kb on + strand at 463.852 kb on + strand at 463.852 kb on + strand at 463.853 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.867 kb on - strand at 463.871 kb on - strand at 463.871 kb on - strand at 463.871 kb on - strand at 463.941 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 460,128 + Psest_0423 0.52 +1.4 460,131 + Psest_0423 0.52 +1.4 460,132 - Psest_0423 0.52 +2.1 460,149 - Psest_0423 0.53 -2.0 460,189 + Psest_0423 0.55 +1.0 460,320 - Psest_0423 0.62 +0.1 460,349 + Psest_0423 0.63 -0.4 460,419 - Psest_0423 0.66 -2.9 460,539 + Psest_0423 0.72 -1.4 460,540 - Psest_0423 0.72 +2.6 460,543 + Psest_0423 0.72 -2.7 460,543 + Psest_0423 0.72 -1.4 460,543 + Psest_0423 0.72 -1.9 460,563 + Psest_0423 0.73 +1.3 460,563 + Psest_0423 0.73 -1.6 460,564 - Psest_0423 0.73 -1.7 460,740 - Psest_0423 0.82 -1.9 460,822 + Psest_0423 0.86 -1.5 460,822 + Psest_0423 0.86 -2.3 460,822 + Psest_0423 0.86 +0.1 460,822 + Psest_0423 0.86 -2.5 460,823 - Psest_0423 0.86 +0.2 460,823 - Psest_0423 0.86 +0.1 460,823 - Psest_0423 0.86 -0.4 460,823 - Psest_0423 0.86 +2.9 460,832 + Psest_0423 0.86 -1.8 460,871 - Psest_0423 0.88 -0.3 460,941 - -1.0 460,998 + +1.9 461,448 + Psest_0424 0.18 -1.0 461,598 + Psest_0424 0.26 -1.2 461,613 + Psest_0424 0.27 -2.1 461,664 + Psest_0424 0.30 +1.5 461,679 + Psest_0424 0.31 +0.5 461,744 + Psest_0424 0.34 -1.4 461,823 - Psest_0424 0.38 -1.7 461,869 + Psest_0424 0.41 +2.8 461,870 - Psest_0424 0.41 +0.1 461,919 + Psest_0424 0.44 -1.5 461,963 - Psest_0424 0.46 +1.5 461,987 + Psest_0424 0.47 +3.9 462,075 + Psest_0424 0.52 +0.3 462,075 + Psest_0424 0.52 +3.0 462,261 + Psest_0424 0.62 +1.1 462,262 - Psest_0424 0.62 +3.2 462,432 + Psest_0424 0.71 +1.4 462,600 + Psest_0424 0.80 -1.2 462,636 + Psest_0424 0.82 -0.6 462,636 + Psest_0424 0.82 -2.9 462,648 + Psest_0424 0.82 +2.1 462,648 + Psest_0424 0.82 +1.7 462,669 + Psest_0424 0.84 -0.6 462,692 + Psest_0424 0.85 -2.0 462,692 + Psest_0424 0.85 -0.9 462,735 + Psest_0424 0.87 -0.3 462,825 + +2.2 462,850 - -0.0 463,103 + +4.3 463,103 + +1.4 463,195 + +2.0 463,340 + +0.8 463,340 + -3.2 463,361 + -1.2 463,361 + -0.0 463,363 + -3.9 463,453 + +2.8 463,497 + +4.1 463,570 + -0.2 463,570 + +1.7 463,570 + -1.6 463,746 + +0.9 463,796 + +1.0 463,797 - +0.8 463,836 + -1.2 463,852 + +3.9 463,852 + +0.5 463,853 - -2.5 463,867 - -0.7 463,867 - -0.0 463,867 - -2.5 463,867 - -0.5 463,867 - -1.5 463,867 - +0.4 463,867 - -1.1 463,867 - -1.4 463,867 - +2.4 463,867 - -1.0 463,867 - +1.9 463,871 - -1.0 463,871 - -1.0 463,871 - -2.3 463,941 + +1.1
Or see this region's nucleotide sequence