Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0039 and Psest_0040 are separated by 248 nucleotides Psest_0040 and Psest_0041 are separated by 184 nucleotides Psest_0041 and Psest_0042 are separated by 118 nucleotides
Psest_0039: Psest_0039 - phosphoglucomutase, alpha-D-glucose phosphate-specific, at 41,591 to 43,255
_0039
Psest_0040: Psest_0040 - Protein of unknown function (DUF2934)., at 43,504 to 43,701
_0040
Psest_0041: Psest_0041 - Uncharacterized protein conserved in bacteria, at 43,886 to 45,367
_0041
Psest_0042: Psest_0042 - Transcriptional regulator, at 45,486 to 46,388
_0042
Position (kb) 43
44
45
46 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 43.176 kb on + strand at 43.176 kb on + strand at 43.176 kb on + strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.177 kb on - strand at 43.337 kb on + strand at 43.338 kb on - strand at 43.347 kb on + strand at 43.347 kb on + strand at 43.348 kb on - strand at 43.537 kb on + strand, within Psest_0040 at 43.538 kb on - strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.621 kb on + strand, within Psest_0040 at 43.622 kb on + strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.622 kb on - strand, within Psest_0040 at 43.699 kb on + strand at 43.818 kb on + strand at 43.818 kb on + strand at 43.819 kb on - strand at 43.819 kb on - strand at 43.914 kb on + strand at 43.915 kb on - strand at 43.946 kb on + strand at 43.947 kb on - strand at 44.093 kb on + strand, within Psest_0041 at 44.339 kb on + strand, within Psest_0041 at 44.558 kb on + strand, within Psest_0041 at 44.558 kb on + strand, within Psest_0041 at 44.558 kb on + strand, within Psest_0041 at 44.559 kb on - strand, within Psest_0041 at 44.559 kb on - strand, within Psest_0041 at 44.559 kb on - strand, within Psest_0041 at 45.263 kb on + strand at 45.263 kb on + strand at 45.263 kb on + strand at 45.264 kb on - strand at 45.484 kb on - strand at 45.574 kb on + strand at 45.574 kb on + strand at 45.574 kb on + strand at 45.574 kb on + strand at 45.574 kb on - strand at 45.575 kb on - strand at 45.575 kb on - strand at 45.575 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 43,176 + +1.6 43,176 + +0.4 43,176 + +0.3 43,177 - -0.3 43,177 - -2.7 43,177 - -0.3 43,177 - -2.8 43,177 - -0.5 43,177 - +1.6 43,337 + +1.3 43,338 - -0.6 43,347 + +2.9 43,347 + +1.3 43,348 - -1.7 43,537 + Psest_0040 0.17 +0.8 43,538 - Psest_0040 0.17 -2.5 43,621 + Psest_0040 0.59 -0.9 43,621 + Psest_0040 0.59 -1.5 43,621 + Psest_0040 0.59 +0.6 43,621 + Psest_0040 0.59 -0.3 43,621 + Psest_0040 0.59 -0.4 43,621 + Psest_0040 0.59 +0.2 43,621 + Psest_0040 0.59 -1.2 43,621 + Psest_0040 0.59 +1.7 43,621 + Psest_0040 0.59 +2.1 43,622 + Psest_0040 0.60 -0.7 43,622 - Psest_0040 0.60 +1.0 43,622 - Psest_0040 0.60 -3.7 43,622 - Psest_0040 0.60 -1.9 43,622 - Psest_0040 0.60 -1.9 43,622 - Psest_0040 0.60 -2.3 43,622 - Psest_0040 0.60 -1.2 43,622 - Psest_0040 0.60 +0.6 43,699 + -2.8 43,818 + +0.7 43,818 + +0.5 43,819 - -2.0 43,819 - +2.2 43,914 + -2.0 43,915 - -0.7 43,946 + +2.9 43,947 - -0.6 44,093 + Psest_0041 0.14 +2.4 44,339 + Psest_0041 0.31 +0.9 44,558 + Psest_0041 0.45 +1.3 44,558 + Psest_0041 0.45 -0.8 44,558 + Psest_0041 0.45 +3.9 44,559 - Psest_0041 0.45 -2.1 44,559 - Psest_0041 0.45 -0.8 44,559 - Psest_0041 0.45 +1.2 45,263 + -1.5 45,263 + +3.7 45,263 + +0.1 45,264 - -2.6 45,484 - -0.8 45,574 + -1.1 45,574 + -0.0 45,574 + -2.7 45,574 + -0.6 45,574 - -0.2 45,575 - -0.6 45,575 - -2.4 45,575 - -0.3
Or see this region's nucleotide sequence