Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0382 and Psest_0383 are separated by 2 nucleotides Psest_0383 and Psest_0384 are separated by 108 nucleotides Psest_0384 and Psest_0385 are separated by 313 nucleotides Psest_0385 and Psest_0386 are separated by 0 nucleotides
Psest_0382: Psest_0382 - Uncharacterized iron-regulated membrane protein, at 411,287 to 412,813
_0382
Psest_0383: Psest_0383 - Protein of unknown function (DUF3325)., at 412,816 to 413,139
_0383
Psest_0384: Psest_0384 - outer membrane porin, OprD family., at 413,248 to 414,519
_0384
Psest_0385: Psest_0385 - Conserved secreted protein, at 414,833 to 415,336
_0385
Psest_0386: Psest_0386 - Membrane protease subunits, stomatin/prohibitin homologs, at 415,337 to 416,095
_0386
Position (kb) 413
414
415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 412.292 kb on + strand, within Psest_0382 at 412.292 kb on + strand, within Psest_0382 at 412.293 kb on - strand, within Psest_0382 at 412.293 kb on - strand, within Psest_0382 at 413.015 kb on + strand, within Psest_0383 at 413.138 kb on - strand at 413.250 kb on - strand at 413.267 kb on + strand at 413.310 kb on - strand at 413.377 kb on - strand, within Psest_0384 at 413.377 kb on - strand, within Psest_0384 at 413.378 kb on + strand, within Psest_0384 at 413.379 kb on - strand, within Psest_0384 at 413.454 kb on - strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.501 kb on + strand, within Psest_0384 at 413.502 kb on - strand, within Psest_0384 at 413.502 kb on - strand, within Psest_0384 at 413.502 kb on - strand, within Psest_0384 at 413.543 kb on + strand, within Psest_0384 at 413.543 kb on + strand, within Psest_0384 at 413.639 kb on + strand, within Psest_0384 at 413.639 kb on + strand, within Psest_0384 at 413.640 kb on - strand, within Psest_0384 at 413.681 kb on + strand, within Psest_0384 at 413.681 kb on + strand, within Psest_0384 at 413.681 kb on + strand, within Psest_0384 at 413.681 kb on + strand, within Psest_0384 at 413.682 kb on - strand, within Psest_0384 at 413.682 kb on - strand, within Psest_0384 at 413.682 kb on - strand, within Psest_0384 at 413.738 kb on + strand, within Psest_0384 at 413.738 kb on + strand, within Psest_0384 at 413.739 kb on - strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.801 kb on + strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.802 kb on - strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.813 kb on + strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.814 kb on - strand, within Psest_0384 at 413.870 kb on + strand, within Psest_0384 at 414.190 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.212 kb on + strand, within Psest_0384 at 414.213 kb on - strand, within Psest_0384 at 414.290 kb on + strand, within Psest_0384 at 414.290 kb on + strand, within Psest_0384 at 414.380 kb on + strand, within Psest_0384 at 414.380 kb on + strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.381 kb on - strand, within Psest_0384 at 414.520 kb on + strand at 414.690 kb on + strand at 414.691 kb on - strand at 414.719 kb on - strand at 414.719 kb on - strand at 414.771 kb on - strand at 414.847 kb on - strand at 415.128 kb on + strand, within Psest_0385 at 415.129 kb on - strand, within Psest_0385 at 415.129 kb on - strand, within Psest_0385 at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.328 kb on + strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.329 kb on - strand at 415.443 kb on + strand, within Psest_0386 at 415.443 kb on + strand, within Psest_0386 at 415.444 kb on - strand, within Psest_0386
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 412,292 + Psest_0382 0.66 -2.4 412,292 + Psest_0382 0.66 -2.4 412,293 - Psest_0382 0.66 -2.0 412,293 - Psest_0382 0.66 -3.2 413,015 + Psest_0383 0.61 -2.8 413,138 - -1.0 413,250 - +0.7 413,267 + -1.1 413,310 - -0.2 413,377 - Psest_0384 0.10 -3.3 413,377 - Psest_0384 0.10 -0.2 413,378 + Psest_0384 0.10 -1.7 413,379 - Psest_0384 0.10 -0.9 413,454 - Psest_0384 0.16 -1.3 413,501 + Psest_0384 0.20 +1.2 413,501 + Psest_0384 0.20 -1.7 413,501 + Psest_0384 0.20 +2.7 413,501 + Psest_0384 0.20 +1.7 413,501 + Psest_0384 0.20 +0.1 413,502 - Psest_0384 0.20 +2.1 413,502 - Psest_0384 0.20 -2.1 413,502 - Psest_0384 0.20 -1.5 413,543 + Psest_0384 0.23 -1.3 413,543 + Psest_0384 0.23 +1.2 413,639 + Psest_0384 0.31 +1.2 413,639 + Psest_0384 0.31 +0.7 413,640 - Psest_0384 0.31 -0.1 413,681 + Psest_0384 0.34 -0.1 413,681 + Psest_0384 0.34 +1.5 413,681 + Psest_0384 0.34 +2.3 413,681 + Psest_0384 0.34 +2.3 413,682 - Psest_0384 0.34 +0.2 413,682 - Psest_0384 0.34 +0.0 413,682 - Psest_0384 0.34 +1.0 413,738 + Psest_0384 0.39 +2.2 413,738 + Psest_0384 0.39 +0.4 413,739 - Psest_0384 0.39 -2.0 413,801 + Psest_0384 0.43 -0.5 413,801 + Psest_0384 0.43 +0.7 413,801 + Psest_0384 0.43 +2.6 413,801 + Psest_0384 0.43 -1.9 413,801 + Psest_0384 0.43 +1.0 413,802 - Psest_0384 0.44 +5.9 413,802 - Psest_0384 0.44 -0.0 413,802 - Psest_0384 0.44 +0.8 413,802 - Psest_0384 0.44 +0.6 413,802 - Psest_0384 0.44 +0.1 413,802 - Psest_0384 0.44 +1.2 413,813 + Psest_0384 0.44 +0.4 413,813 + Psest_0384 0.44 -1.1 413,813 + Psest_0384 0.44 -3.2 413,813 + Psest_0384 0.44 +1.4 413,813 + Psest_0384 0.44 -0.0 413,814 - Psest_0384 0.44 -0.7 413,814 - Psest_0384 0.44 -0.8 413,814 - Psest_0384 0.44 +0.3 413,814 - Psest_0384 0.44 -0.4 413,814 - Psest_0384 0.44 -0.6 413,814 - Psest_0384 0.44 -2.4 413,814 - Psest_0384 0.44 -0.8 413,814 - Psest_0384 0.44 +2.6 413,814 - Psest_0384 0.44 +0.3 413,814 - Psest_0384 0.44 -1.6 413,814 - Psest_0384 0.44 -0.8 413,870 + Psest_0384 0.49 +1.6 414,190 + Psest_0384 0.74 +1.0 414,212 + Psest_0384 0.76 +0.3 414,212 + Psest_0384 0.76 +0.3 414,212 + Psest_0384 0.76 +1.0 414,213 - Psest_0384 0.76 +0.7 414,290 + Psest_0384 0.82 -0.0 414,290 + Psest_0384 0.82 +1.0 414,380 + Psest_0384 0.89 +0.5 414,380 + Psest_0384 0.89 -0.4 414,381 - Psest_0384 0.89 -2.3 414,381 - Psest_0384 0.89 -2.1 414,381 - Psest_0384 0.89 -0.7 414,381 - Psest_0384 0.89 -1.8 414,381 - Psest_0384 0.89 -2.3 414,520 + +1.1 414,690 + +0.5 414,691 - -0.5 414,719 - +0.2 414,719 - +0.6 414,771 - +0.4 414,847 - -1.5 415,128 + Psest_0385 0.59 +0.7 415,129 - Psest_0385 0.59 +0.6 415,129 - Psest_0385 0.59 +0.2 415,328 + +2.0 415,328 + +2.8 415,328 + +2.1 415,328 + -1.0 415,328 + -1.3 415,328 + -1.4 415,329 - +0.2 415,329 - +0.7 415,329 - -0.4 415,329 - +2.3 415,329 - +0.5 415,329 - -1.9 415,329 - +1.7 415,329 - +0.7 415,329 - +4.1 415,329 - -1.3 415,329 - +0.9 415,329 - +0.8 415,443 + Psest_0386 0.14 -0.1 415,443 + Psest_0386 0.14 -1.2 415,444 - Psest_0386 0.14 +0.6
Or see this region's nucleotide sequence