Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0354 and Psest_0355 are separated by 219 nucleotides Psest_0355 and Psest_0356 are separated by 15 nucleotides Psest_0356 and Psest_0357 are separated by 202 nucleotides Psest_0357 and Psest_0358 overlap by 4 nucleotides
Psest_0354: Psest_0354 - Malate synthase G (EC 2.3.3.9) <small>(from data)</small>, at 379,042 to 381,222
_0354
Psest_0355: Psest_0355 - Glycine cleavage system regulatory protein, at 381,442 to 381,966
_0355
Psest_0356: Psest_0356 - rarD protein, at 381,982 to 382,872
_0356
Psest_0357: Psest_0357 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 383,075 to 383,842
_0357
Psest_0358: Psest_0358 - Neutral trehalase, at 383,839 to 385,443
_0358
Position (kb) 381
382
383 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 380.982 kb on - strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 380.982 kb on - strand, within Psest_0354 at 381.173 kb on + strand at 381.245 kb on + strand at 381.245 kb on + strand at 381.246 kb on - strand at 381.246 kb on - strand at 381.246 kb on - strand at 381.272 kb on + strand at 381.289 kb on + strand at 381.289 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.292 kb on + strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.293 kb on - strand at 381.386 kb on + strand at 381.386 kb on + strand at 381.387 kb on - strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.388 kb on + strand at 381.389 kb on - strand at 381.392 kb on + strand at 381.392 kb on + strand at 381.392 kb on + strand at 381.392 kb on + strand at 381.392 kb on + strand at 381.393 kb on - strand at 381.528 kb on + strand, within Psest_0355 at 381.629 kb on + strand, within Psest_0355 at 381.713 kb on + strand, within Psest_0355 at 381.996 kb on + strand at 381.996 kb on + strand at 381.996 kb on + strand at 381.997 kb on - strand at 381.997 kb on - strand at 382.025 kb on + strand at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.553 kb on + strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.554 kb on - strand, within Psest_0356 at 382.556 kb on + strand, within Psest_0356 at 382.556 kb on + strand, within Psest_0356 at 382.557 kb on - strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.604 kb on + strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.605 kb on - strand, within Psest_0356 at 382.715 kb on - strand, within Psest_0356 at 382.796 kb on + strand at 382.797 kb on - strand at 382.844 kb on + strand at 382.938 kb on - strand at 382.950 kb on + strand at 382.950 kb on + strand at 382.950 kb on + strand at 382.951 kb on - strand at 383.027 kb on + strand at 383.027 kb on + strand at 383.224 kb on - strand, within Psest_0357 at 383.224 kb on - strand, within Psest_0357 at 383.224 kb on - strand, within Psest_0357 at 383.394 kb on - strand, within Psest_0357 at 383.394 kb on - strand, within Psest_0357 at 383.541 kb on - strand, within Psest_0357 at 383.780 kb on + strand at 383.780 kb on + strand at 383.780 kb on + strand at 383.780 kb on + strand at 383.780 kb on + strand at 383.781 kb on - strand at 383.781 kb on - strand at 383.781 kb on - strand at 383.781 kb on - strand at 383.781 kb on - strand at 383.781 kb on - strand at 383.781 kb on - strand at 383.781 kb on - strand at 383.820 kb on + strand at 383.828 kb on + strand at 383.829 kb on - strand at 383.829 kb on - strand at 383.829 kb on - strand at 383.829 kb on - strand at 383.829 kb on - strand at 383.829 kb on - strand at 383.872 kb on + strand at 383.872 kb on + strand at 383.872 kb on + strand at 383.872 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 380,982 - Psest_0354 0.89 +1.2 380,982 - Psest_0354 0.89 +1.3 380,982 - Psest_0354 0.89 +0.4 380,982 - Psest_0354 0.89 -1.6 380,982 - Psest_0354 0.89 +0.9 381,173 + +1.6 381,245 + +2.7 381,245 + +0.5 381,246 - -2.1 381,246 - -1.1 381,246 - +5.0 381,272 + +0.2 381,289 + +1.2 381,289 + +0.1 381,292 + -0.0 381,292 + +0.6 381,292 + +1.8 381,292 + -2.2 381,292 + -3.0 381,292 + +0.9 381,292 + +1.3 381,293 - -1.8 381,293 - +0.8 381,293 - +0.4 381,293 - +1.9 381,293 - +0.7 381,293 - +4.0 381,293 - +0.2 381,293 - +1.8 381,293 - -1.9 381,293 - -0.2 381,293 - +1.8 381,293 - -1.0 381,293 - +4.2 381,293 - -1.0 381,293 - -1.5 381,293 - -0.9 381,386 + -0.5 381,386 + +1.2 381,387 - -2.8 381,388 + +0.7 381,388 + -1.6 381,388 + -0.6 381,388 + +1.5 381,388 + +1.1 381,388 + +1.0 381,388 + +2.4 381,388 + -0.2 381,388 + -0.0 381,388 + +2.2 381,388 + -0.2 381,389 - +1.1 381,392 + +1.3 381,392 + +1.2 381,392 + -0.5 381,392 + +1.0 381,392 + +2.0 381,393 - -2.9 381,528 + Psest_0355 0.16 +0.0 381,629 + Psest_0355 0.36 +0.9 381,713 + Psest_0355 0.52 -0.8 381,996 + -2.1 381,996 + +2.1 381,996 + -0.4 381,997 - -0.2 381,997 - +3.8 382,025 + -2.7 382,553 + Psest_0356 0.64 +4.9 382,553 + Psest_0356 0.64 -1.3 382,553 + Psest_0356 0.64 +1.9 382,553 + Psest_0356 0.64 +0.9 382,553 + Psest_0356 0.64 +0.4 382,553 + Psest_0356 0.64 +2.5 382,553 + Psest_0356 0.64 -0.8 382,553 + Psest_0356 0.64 -0.4 382,553 + Psest_0356 0.64 +1.3 382,553 + Psest_0356 0.64 -0.3 382,553 + Psest_0356 0.64 -2.3 382,553 + Psest_0356 0.64 +1.8 382,554 - Psest_0356 0.64 -1.0 382,554 - Psest_0356 0.64 -4.1 382,554 - Psest_0356 0.64 +2.3 382,554 - Psest_0356 0.64 +0.6 382,554 - Psest_0356 0.64 +1.8 382,554 - Psest_0356 0.64 +0.6 382,556 + Psest_0356 0.64 -2.6 382,556 + Psest_0356 0.64 -0.1 382,557 - Psest_0356 0.65 -0.3 382,604 + Psest_0356 0.70 -0.6 382,604 + Psest_0356 0.70 -1.8 382,604 + Psest_0356 0.70 +0.1 382,604 + Psest_0356 0.70 -2.6 382,604 + Psest_0356 0.70 +1.0 382,604 + Psest_0356 0.70 -0.5 382,604 + Psest_0356 0.70 +2.8 382,604 + Psest_0356 0.70 +0.1 382,604 + Psest_0356 0.70 -0.0 382,604 + Psest_0356 0.70 +1.2 382,605 - Psest_0356 0.70 -1.2 382,605 - Psest_0356 0.70 -2.1 382,605 - Psest_0356 0.70 +4.1 382,605 - Psest_0356 0.70 -3.2 382,605 - Psest_0356 0.70 -1.1 382,605 - Psest_0356 0.70 +0.5 382,605 - Psest_0356 0.70 +2.8 382,605 - Psest_0356 0.70 +1.4 382,605 - Psest_0356 0.70 +0.2 382,605 - Psest_0356 0.70 +1.5 382,605 - Psest_0356 0.70 +0.3 382,605 - Psest_0356 0.70 +1.7 382,605 - Psest_0356 0.70 +0.5 382,605 - Psest_0356 0.70 +1.7 382,715 - Psest_0356 0.82 -0.5 382,796 + -0.2 382,797 - -0.3 382,844 + +3.7 382,938 - -0.2 382,950 + +2.8 382,950 + -1.3 382,950 + +2.8 382,951 - -1.9 383,027 + +0.4 383,027 + -0.3 383,224 - Psest_0357 0.19 +0.5 383,224 - Psest_0357 0.19 -1.3 383,224 - Psest_0357 0.19 -2.2 383,394 - Psest_0357 0.42 -1.2 383,394 - Psest_0357 0.42 -1.2 383,541 - Psest_0357 0.61 -1.9 383,780 + -2.3 383,780 + -2.2 383,780 + -2.6 383,780 + +1.9 383,780 + +2.1 383,781 - +3.1 383,781 - +0.1 383,781 - -1.2 383,781 - +1.1 383,781 - -0.6 383,781 - +0.8 383,781 - +1.1 383,781 - +0.5 383,820 + -0.0 383,828 + -2.5 383,829 - -2.1 383,829 - +0.4 383,829 - -1.3 383,829 - -0.0 383,829 - +1.6 383,829 - +1.4 383,872 + -1.4 383,872 + -0.5 383,872 + +2.7 383,872 + -0.1
Or see this region's nucleotide sequence