Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0286

Experiment: RCH2 grown on a colony of ecoli

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0285 and Psest_0286 are separated by 59 nucleotidesPsest_0286 and Psest_0287 overlap by 4 nucleotidesPsest_0287 and Psest_0288 overlap by 1 nucleotides Psest_0285: Psest_0285 - pilus retraction protein PilT, at 300,340 to 301,374 _0285 Psest_0286: Psest_0286 - pilus retraction protein PilT, at 301,434 to 302,579 _0286 Psest_0287: Psest_0287 - probable DNA metabolism protein, at 302,576 to 303,412 _0287 Psest_0288: Psest_0288 - putative DNA modification/repair radical SAM protein, at 303,412 to 304,623 _0288 Position (kb) 301 302 303Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4 5at 300.493 kb on + strand, within Psest_0285at 300.493 kb on + strand, within Psest_0285at 300.493 kb on + strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.494 kb on - strand, within Psest_0285at 300.776 kb on - strand, within Psest_0285at 300.793 kb on + strand, within Psest_0285at 300.793 kb on + strand, within Psest_0285at 300.794 kb on - strand, within Psest_0285at 300.794 kb on - strand, within Psest_0285at 300.794 kb on - strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.243 kb on + strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.244 kb on - strand, within Psest_0285at 301.323 kb on + strandat 301.323 kb on + strandat 301.323 kb on + strandat 301.323 kb on + strandat 301.324 kb on - strandat 301.372 kb on + strandat 301.373 kb on - strandat 301.427 kb on + strandat 301.428 kb on - strandat 301.693 kb on - strand, within Psest_0286at 301.693 kb on - strand, within Psest_0286at 302.070 kb on + strand, within Psest_0286at 302.070 kb on + strand, within Psest_0286at 302.071 kb on - strand, within Psest_0286at 302.071 kb on - strand, within Psest_0286at 302.071 kb on - strand, within Psest_0286at 302.397 kb on + strand, within Psest_0286at 302.397 kb on + strand, within Psest_0286at 302.397 kb on + strand, within Psest_0286at 302.398 kb on - strand, within Psest_0286at 302.398 kb on - strand, within Psest_0286at 302.398 kb on - strand, within Psest_0286at 302.398 kb on - strand, within Psest_0286at 302.406 kb on + strand, within Psest_0286at 302.406 kb on + strand, within Psest_0286at 302.406 kb on + strand, within Psest_0286at 302.406 kb on + strand, within Psest_0286at 302.406 kb on + strand, within Psest_0286at 302.406 kb on + strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.407 kb on - strand, within Psest_0286at 302.427 kb on + strand, within Psest_0286at 302.427 kb on + strand, within Psest_0286at 302.428 kb on - strand, within Psest_0286at 302.428 kb on - strand, within Psest_0286at 302.428 kb on - strand, within Psest_0286at 302.428 kb on - strand, within Psest_0286at 302.428 kb on - strand, within Psest_0286at 302.784 kb on + strand, within Psest_0287at 302.784 kb on + strand, within Psest_0287at 302.784 kb on + strand, within Psest_0287at 302.785 kb on - strand, within Psest_0287at 302.787 kb on + strand, within Psest_0287at 302.787 kb on + strand, within Psest_0287at 302.787 kb on + strand, within Psest_0287at 302.787 kb on + strand, within Psest_0287at 302.787 kb on + strand, within Psest_0287at 302.787 kb on + strand, within Psest_0287at 302.787 kb on + strand, within Psest_0287at 302.787 kb on + strand, within Psest_0287at 302.788 kb on - strand, within Psest_0287at 302.788 kb on - strand, within Psest_0287at 302.788 kb on - strand, within Psest_0287at 302.788 kb on - strand, within Psest_0287at 302.788 kb on - strand, within Psest_0287at 302.788 kb on - strand, within Psest_0287at 302.788 kb on - strand, within Psest_0287at 302.788 kb on - strand, within Psest_0287at 302.799 kb on - strand, within Psest_0287at 302.865 kb on + strand, within Psest_0287at 302.865 kb on + strand, within Psest_0287at 302.865 kb on + strand, within Psest_0287at 302.865 kb on + strand, within Psest_0287at 302.865 kb on + strand, within Psest_0287at 302.865 kb on + strand, within Psest_0287at 302.866 kb on - strand, within Psest_0287at 302.866 kb on - strand, within Psest_0287at 302.866 kb on - strand, within Psest_0287at 302.866 kb on - strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.868 kb on + strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.869 kb on - strand, within Psest_0287at 302.969 kb on + strand, within Psest_0287at 302.969 kb on + strand, within Psest_0287at 302.969 kb on + strand, within Psest_0287at 302.969 kb on + strand, within Psest_0287at 302.970 kb on - strand, within Psest_0287at 302.970 kb on - strand, within Psest_0287at 303.092 kb on + strand, within Psest_0287at 303.092 kb on + strand, within Psest_0287at 303.092 kb on + strand, within Psest_0287at 303.092 kb on + strand, within Psest_0287at 303.092 kb on + strand, within Psest_0287at 303.093 kb on - strand, within Psest_0287at 303.093 kb on - strand, within Psest_0287at 303.093 kb on - strand, within Psest_0287at 303.093 kb on - strand, within Psest_0287at 303.542 kb on - strand, within Psest_0288at 303.542 kb on - strand, within Psest_0288

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Per-strain Table

Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli
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300,493 + Psest_0285 0.15 -1.2
300,493 + Psest_0285 0.15 -0.0
300,493 + Psest_0285 0.15 +0.4
300,494 - Psest_0285 0.15 -3.9
300,494 - Psest_0285 0.15 -0.3
300,494 - Psest_0285 0.15 +1.4
300,494 - Psest_0285 0.15 -0.9
300,494 - Psest_0285 0.15 +0.6
300,494 - Psest_0285 0.15 -0.9
300,776 - Psest_0285 0.42 -0.2
300,793 + Psest_0285 0.44 -0.3
300,793 + Psest_0285 0.44 -0.3
300,794 - Psest_0285 0.44 -0.8
300,794 - Psest_0285 0.44 -0.5
300,794 - Psest_0285 0.44 +0.4
301,243 + Psest_0285 0.87 -0.9
301,243 + Psest_0285 0.87 -0.3
301,243 + Psest_0285 0.87 +1.0
301,243 + Psest_0285 0.87 +1.8
301,243 + Psest_0285 0.87 -1.9
301,243 + Psest_0285 0.87 +0.4
301,243 + Psest_0285 0.87 -1.2
301,243 + Psest_0285 0.87 +0.8
301,243 + Psest_0285 0.87 +3.0
301,243 + Psest_0285 0.87 -0.3
301,244 - Psest_0285 0.87 -0.3
301,244 - Psest_0285 0.87 +1.2
301,244 - Psest_0285 0.87 -0.1
301,244 - Psest_0285 0.87 +0.7
301,244 - Psest_0285 0.87 +1.6
301,244 - Psest_0285 0.87 +0.3
301,244 - Psest_0285 0.87 -0.1
301,244 - Psest_0285 0.87 +2.6
301,244 - Psest_0285 0.87 +0.9
301,244 - Psest_0285 0.87 +1.1
301,244 - Psest_0285 0.87 +0.5
301,323 + +0.5
301,323 + +1.2
301,323 + +0.3
301,323 + +1.9
301,324 - +0.6
301,372 + -2.5
301,373 - -2.3
301,427 + -2.2
301,428 - -1.8
301,693 - Psest_0286 0.23 -0.2
301,693 - Psest_0286 0.23 +0.9
302,070 + Psest_0286 0.55 +0.6
302,070 + Psest_0286 0.55 +0.4
302,071 - Psest_0286 0.56 +0.8
302,071 - Psest_0286 0.56 -2.7
302,071 - Psest_0286 0.56 -1.2
302,397 + Psest_0286 0.84 -1.2
302,397 + Psest_0286 0.84 -2.2
302,397 + Psest_0286 0.84 +0.1
302,398 - Psest_0286 0.84 +0.8
302,398 - Psest_0286 0.84 -1.6
302,398 - Psest_0286 0.84 -1.5
302,398 - Psest_0286 0.84 -0.1
302,406 + Psest_0286 0.85 -1.3
302,406 + Psest_0286 0.85 -0.8
302,406 + Psest_0286 0.85 -1.0
302,406 + Psest_0286 0.85 -1.5
302,406 + Psest_0286 0.85 -1.5
302,406 + Psest_0286 0.85 -1.2
302,407 - Psest_0286 0.85 -1.3
302,407 - Psest_0286 0.85 -0.3
302,407 - Psest_0286 0.85 -1.5
302,407 - Psest_0286 0.85 +0.5
302,407 - Psest_0286 0.85 -2.3
302,407 - Psest_0286 0.85 +2.4
302,407 - Psest_0286 0.85 -0.4
302,407 - Psest_0286 0.85 +3.0
302,407 - Psest_0286 0.85 +2.5
302,407 - Psest_0286 0.85 -0.9
302,427 + Psest_0286 0.87 +0.4
302,427 + Psest_0286 0.87 +1.5
302,428 - Psest_0286 0.87 -1.1
302,428 - Psest_0286 0.87 -0.9
302,428 - Psest_0286 0.87 -0.4
302,428 - Psest_0286 0.87 -2.2
302,428 - Psest_0286 0.87 +0.0
302,784 + Psest_0287 0.25 +0.8
302,784 + Psest_0287 0.25 -0.0
302,784 + Psest_0287 0.25 -0.3
302,785 - Psest_0287 0.25 -0.1
302,787 + Psest_0287 0.25 +4.2
302,787 + Psest_0287 0.25 -2.7
302,787 + Psest_0287 0.25 +1.0
302,787 + Psest_0287 0.25 +1.7
302,787 + Psest_0287 0.25 -0.4
302,787 + Psest_0287 0.25 -1.6
302,787 + Psest_0287 0.25 +0.7
302,787 + Psest_0287 0.25 -0.3
302,788 - Psest_0287 0.25 -0.7
302,788 - Psest_0287 0.25 -1.3
302,788 - Psest_0287 0.25 +0.8
302,788 - Psest_0287 0.25 +1.4
302,788 - Psest_0287 0.25 -1.5
302,788 - Psest_0287 0.25 -0.4
302,788 - Psest_0287 0.25 +0.0
302,788 - Psest_0287 0.25 +0.5
302,799 - Psest_0287 0.27 -3.0
302,865 + Psest_0287 0.35 -2.1
302,865 + Psest_0287 0.35 +0.9
302,865 + Psest_0287 0.35 +0.3
302,865 + Psest_0287 0.35 -0.3
302,865 + Psest_0287 0.35 -0.3
302,865 + Psest_0287 0.35 +1.8
302,866 - Psest_0287 0.35 -2.5
302,866 - Psest_0287 0.35 +0.4
302,866 - Psest_0287 0.35 -2.8
302,866 - Psest_0287 0.35 +5.2
302,868 + Psest_0287 0.35 +1.8
302,868 + Psest_0287 0.35 -1.8
302,868 + Psest_0287 0.35 +0.6
302,868 + Psest_0287 0.35 -0.1
302,868 + Psest_0287 0.35 -2.6
302,868 + Psest_0287 0.35 -1.8
302,868 + Psest_0287 0.35 -3.2
302,868 + Psest_0287 0.35 -0.9
302,868 + Psest_0287 0.35 +0.9
302,868 + Psest_0287 0.35 +1.0
302,868 + Psest_0287 0.35 +4.2
302,869 - Psest_0287 0.35 -1.1
302,869 - Psest_0287 0.35 +3.5
302,869 - Psest_0287 0.35 +0.2
302,869 - Psest_0287 0.35 -2.3
302,869 - Psest_0287 0.35 -2.1
302,869 - Psest_0287 0.35 +0.1
302,869 - Psest_0287 0.35 +1.8
302,869 - Psest_0287 0.35 +1.6
302,869 - Psest_0287 0.35 +1.6
302,869 - Psest_0287 0.35 +1.6
302,869 - Psest_0287 0.35 +0.5
302,869 - Psest_0287 0.35 +1.0
302,869 - Psest_0287 0.35 -0.8
302,869 - Psest_0287 0.35 +0.9
302,969 + Psest_0287 0.47 +2.8
302,969 + Psest_0287 0.47 +1.4
302,969 + Psest_0287 0.47 +0.6
302,969 + Psest_0287 0.47 +3.1
302,970 - Psest_0287 0.47 -0.7
302,970 - Psest_0287 0.47 +0.4
303,092 + Psest_0287 0.62 +1.5
303,092 + Psest_0287 0.62 -1.2
303,092 + Psest_0287 0.62 -0.8
303,092 + Psest_0287 0.62 -2.3
303,092 + Psest_0287 0.62 +1.2
303,093 - Psest_0287 0.62 -1.4
303,093 - Psest_0287 0.62 -3.0
303,093 - Psest_0287 0.62 +0.7
303,093 - Psest_0287 0.62 +0.2
303,542 - Psest_0288 0.11 +0.7
303,542 - Psest_0288 0.11 -0.8

Or see this region's nucleotide sequence