Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0150 and Psest_0151 are separated by 357 nucleotides Psest_0151 and Psest_0152 are separated by 129 nucleotides Psest_0152 and Psest_0153 overlap by 1 nucleotides
Psest_0150: Psest_0150 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 148,970 to 150,301
_0150
Psest_0151: Psest_0151 - Response regulator with putative antiterminator output domain, at 150,659 to 151,969
_0151
Psest_0152: Psest_0152 - Imidazoleglycerol-phosphate dehydratase, at 152,099 to 152,692
_0152
Psest_0153: Psest_0153 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 152,692 to 153,330
_0153
Position (kb) 150
151
152 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 149.829 kb on + strand, within Psest_0150 at 149.935 kb on - strand, within Psest_0150 at 150.032 kb on + strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.054 kb on + strand, within Psest_0150 at 150.054 kb on + strand, within Psest_0150 at 150.055 kb on - strand, within Psest_0150 at 150.055 kb on - strand, within Psest_0150 at 150.725 kb on + strand at 150.725 kb on + strand at 150.726 kb on - strand at 150.726 kb on - strand at 150.789 kb on + strand at 150.790 kb on - strand, within Psest_0151 at 150.790 kb on - strand, within Psest_0151 at 150.790 kb on - strand, within Psest_0151 at 150.912 kb on - strand, within Psest_0151 at 150.912 kb on - strand, within Psest_0151 at 151.299 kb on - strand, within Psest_0151 at 151.654 kb on - strand, within Psest_0151 at 151.654 kb on - strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.810 kb on - strand, within Psest_0151 at 151.862 kb on - strand at 152.058 kb on + strand at 152.058 kb on + strand at 152.059 kb on - strand at 152.059 kb on - strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.061 kb on - strand at 152.061 kb on - strand at 152.111 kb on - strand at 152.111 kb on - strand at 152.111 kb on - strand at 152.117 kb on + strand at 152.117 kb on + strand at 152.118 kb on - strand at 152.118 kb on - strand at 152.118 kb on - strand at 152.121 kb on + strand at 152.122 kb on - strand at 152.122 kb on - strand at 152.122 kb on - strand at 152.339 kb on - strand, within Psest_0152 at 152.339 kb on - strand, within Psest_0152 at 152.387 kb on + strand, within Psest_0152 at 152.387 kb on + strand, within Psest_0152 at 152.388 kb on - strand, within Psest_0152 at 152.388 kb on - strand, within Psest_0152 at 152.465 kb on + strand, within Psest_0152 at 152.466 kb on - strand, within Psest_0152 at 152.466 kb on - strand, within Psest_0152 at 152.555 kb on - strand, within Psest_0152 at 152.690 kb on + strand at 152.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 149,829 + Psest_0150 0.64 +1.0 149,935 - Psest_0150 0.72 +2.7 150,032 + Psest_0150 0.80 -0.6 150,033 - Psest_0150 0.80 +0.3 150,033 - Psest_0150 0.80 -0.8 150,033 - Psest_0150 0.80 +0.7 150,033 - Psest_0150 0.80 +1.7 150,054 + Psest_0150 0.81 +2.7 150,054 + Psest_0150 0.81 -0.2 150,055 - Psest_0150 0.81 -0.4 150,055 - Psest_0150 0.81 -0.4 150,725 + -0.2 150,725 + +0.1 150,726 - -2.2 150,726 - +1.0 150,789 + -0.1 150,790 - Psest_0151 0.10 +0.2 150,790 - Psest_0151 0.10 -1.3 150,790 - Psest_0151 0.10 -1.0 150,912 - Psest_0151 0.19 -0.1 150,912 - Psest_0151 0.19 +0.6 151,299 - Psest_0151 0.49 -1.0 151,654 - Psest_0151 0.76 -0.1 151,654 - Psest_0151 0.76 -0.9 151,809 + Psest_0151 0.88 +1.5 151,809 + Psest_0151 0.88 -1.3 151,809 + Psest_0151 0.88 -2.1 151,809 + Psest_0151 0.88 -0.8 151,809 + Psest_0151 0.88 -1.0 151,809 + Psest_0151 0.88 +0.7 151,810 - Psest_0151 0.88 +3.2 151,862 - +2.4 152,058 + -0.6 152,058 + -0.3 152,059 - +0.3 152,059 - -2.2 152,060 + -1.2 152,060 + +1.0 152,060 + +1.6 152,060 + -0.0 152,060 + +2.5 152,061 - +0.2 152,061 - -0.2 152,111 - -0.0 152,111 - -0.6 152,111 - +1.2 152,117 + +1.3 152,117 + +2.1 152,118 - +1.0 152,118 - +0.6 152,118 - +0.1 152,121 + +2.1 152,122 - +3.7 152,122 - +1.0 152,122 - +2.1 152,339 - Psest_0152 0.40 -0.5 152,339 - Psest_0152 0.40 +0.9 152,387 + Psest_0152 0.48 -1.5 152,387 + Psest_0152 0.48 -0.8 152,388 - Psest_0152 0.49 -0.4 152,388 - Psest_0152 0.49 +3.1 152,465 + Psest_0152 0.62 -0.1 152,466 - Psest_0152 0.62 -0.4 152,466 - Psest_0152 0.62 -2.5 152,555 - Psest_0152 0.77 -1.5 152,690 + -2.5 152,691 - +2.0
Or see this region's nucleotide sequence