Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1154 and Psest_1155 are separated by 225 nucleotides Psest_1155 and Psest_1156 are separated by 81 nucleotides
Psest_1154: Psest_1154 - RND family efflux transporter, MFP subunit, at 1,252,813 to 1,253,946
_1154
Psest_1155: Psest_1155 - Methyl-accepting chemotaxis protein, at 1,254,172 to 1,255,794
_1155
Psest_1156: Psest_1156 - outer membrane component of uptake system, probably for ferrous iron (from data) , at 1,255,876 to 1,256,970
_1156
Position (kb)
1254
1255
1256 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1253.348 kb on + strand, within Psest_1154 at 1253.348 kb on + strand, within Psest_1154 at 1253.349 kb on - strand, within Psest_1154 at 1253.349 kb on - strand, within Psest_1154 at 1253.349 kb on - strand, within Psest_1154 at 1253.400 kb on - strand, within Psest_1154 at 1253.400 kb on - strand, within Psest_1154 at 1253.400 kb on - strand, within Psest_1154 at 1253.400 kb on - strand, within Psest_1154 at 1253.400 kb on - strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.407 kb on + strand, within Psest_1154 at 1253.408 kb on - strand, within Psest_1154 at 1253.408 kb on - strand, within Psest_1154 at 1253.408 kb on - strand, within Psest_1154 at 1253.408 kb on - strand, within Psest_1154 at 1253.408 kb on - strand, within Psest_1154 at 1253.408 kb on - strand, within Psest_1154 at 1253.408 kb on - strand, within Psest_1154 at 1253.408 kb on - strand, within Psest_1154 at 1253.532 kb on - strand, within Psest_1154 at 1253.662 kb on - strand, within Psest_1154 at 1253.864 kb on + strand at 1253.864 kb on + strand at 1253.864 kb on + strand at 1253.865 kb on - strand at 1253.865 kb on - strand at 1254.077 kb on + strand at 1254.078 kb on - strand at 1254.098 kb on + strand at 1254.098 kb on + strand at 1254.098 kb on + strand at 1254.098 kb on + strand at 1254.098 kb on + strand at 1254.099 kb on - strand at 1254.099 kb on - strand at 1254.380 kb on - strand, within Psest_1155 at 1254.380 kb on - strand, within Psest_1155 at 1254.853 kb on + strand, within Psest_1155 at 1255.388 kb on + strand, within Psest_1155 at 1255.388 kb on + strand, within Psest_1155 at 1255.388 kb on + strand, within Psest_1155 at 1255.388 kb on + strand, within Psest_1155 at 1255.388 kb on + strand, within Psest_1155 at 1255.388 kb on + strand, within Psest_1155 at 1255.389 kb on - strand, within Psest_1155 at 1255.389 kb on - strand, within Psest_1155 at 1255.389 kb on - strand, within Psest_1155 at 1255.389 kb on - strand, within Psest_1155 at 1255.389 kb on - strand, within Psest_1155 at 1255.486 kb on + strand, within Psest_1155 at 1255.492 kb on + strand, within Psest_1155 at 1255.492 kb on + strand, within Psest_1155 at 1255.492 kb on + strand, within Psest_1155 at 1255.492 kb on + strand, within Psest_1155 at 1255.492 kb on + strand, within Psest_1155 at 1255.492 kb on + strand, within Psest_1155 at 1255.492 kb on + strand, within Psest_1155 at 1255.493 kb on - strand, within Psest_1155 at 1255.493 kb on - strand, within Psest_1155 at 1255.695 kb on + strand at 1255.808 kb on + strand at 1255.809 kb on - strand at 1255.809 kb on - strand at 1255.809 kb on - strand at 1255.877 kb on + strand at 1255.878 kb on - strand at 1255.878 kb on - strand at 1255.888 kb on + strand at 1255.888 kb on + strand at 1255.888 kb on + strand at 1255.888 kb on + strand at 1255.889 kb on - strand at 1255.889 kb on - strand at 1255.889 kb on - strand at 1255.889 kb on - strand at 1256.088 kb on + strand, within Psest_1156 at 1256.088 kb on + strand, within Psest_1156 at 1256.151 kb on - strand, within Psest_1156 at 1256.258 kb on + strand, within Psest_1156 at 1256.259 kb on - strand, within Psest_1156 at 1256.601 kb on - strand, within Psest_1156 at 1256.601 kb on - strand, within Psest_1156 at 1256.601 kb on - strand, within Psest_1156 at 1256.601 kb on - strand, within Psest_1156 at 1256.640 kb on - strand, within Psest_1156 at 1256.640 kb on - strand, within Psest_1156 at 1256.705 kb on + strand, within Psest_1156 at 1256.705 kb on + strand, within Psest_1156 at 1256.706 kb on - strand, within Psest_1156 at 1256.706 kb on - strand, within Psest_1156 at 1256.706 kb on - strand, within Psest_1156 at 1256.754 kb on - strand, within Psest_1156 at 1256.754 kb on - strand, within Psest_1156
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 1,253,348 + Psest_1154 0.47 +0.8 1,253,348 + Psest_1154 0.47 -0.5 1,253,349 - Psest_1154 0.47 +0.5 1,253,349 - Psest_1154 0.47 -1.6 1,253,349 - Psest_1154 0.47 +0.7 1,253,400 - Psest_1154 0.52 -0.5 1,253,400 - Psest_1154 0.52 +1.1 1,253,400 - Psest_1154 0.52 +0.1 1,253,400 - Psest_1154 0.52 -0.5 1,253,400 - Psest_1154 0.52 -0.3 1,253,407 + Psest_1154 0.52 -2.2 1,253,407 + Psest_1154 0.52 -1.6 1,253,407 + Psest_1154 0.52 +0.2 1,253,407 + Psest_1154 0.52 +0.1 1,253,407 + Psest_1154 0.52 +1.5 1,253,407 + Psest_1154 0.52 +1.8 1,253,407 + Psest_1154 0.52 -1.3 1,253,407 + Psest_1154 0.52 +0.3 1,253,407 + Psest_1154 0.52 -1.2 1,253,408 - Psest_1154 0.52 -1.3 1,253,408 - Psest_1154 0.52 -1.5 1,253,408 - Psest_1154 0.52 -1.2 1,253,408 - Psest_1154 0.52 +0.5 1,253,408 - Psest_1154 0.52 -1.6 1,253,408 - Psest_1154 0.52 -0.9 1,253,408 - Psest_1154 0.52 +0.3 1,253,408 - Psest_1154 0.52 -1.7 1,253,532 - Psest_1154 0.63 +3.1 1,253,662 - Psest_1154 0.75 +1.4 1,253,864 + -0.7 1,253,864 + +0.0 1,253,864 + -1.5 1,253,865 - -1.3 1,253,865 - +1.6 1,254,077 + +2.5 1,254,078 - +0.3 1,254,098 + +1.2 1,254,098 + +1.6 1,254,098 + -1.2 1,254,098 + +0.9 1,254,098 + +0.8 1,254,099 - -2.3 1,254,099 - +0.3 1,254,380 - Psest_1155 0.13 -1.5 1,254,380 - Psest_1155 0.13 -1.1 1,254,853 + Psest_1155 0.42 -2.2 1,255,388 + Psest_1155 0.75 -1.5 1,255,388 + Psest_1155 0.75 -1.4 1,255,388 + Psest_1155 0.75 -0.9 1,255,388 + Psest_1155 0.75 -2.0 1,255,388 + Psest_1155 0.75 +0.8 1,255,388 + Psest_1155 0.75 +1.2 1,255,389 - Psest_1155 0.75 +0.8 1,255,389 - Psest_1155 0.75 -1.9 1,255,389 - Psest_1155 0.75 +0.3 1,255,389 - Psest_1155 0.75 +0.8 1,255,389 - Psest_1155 0.75 -1.2 1,255,486 + Psest_1155 0.81 +0.1 1,255,492 + Psest_1155 0.81 +0.8 1,255,492 + Psest_1155 0.81 -3.2 1,255,492 + Psest_1155 0.81 +1.5 1,255,492 + Psest_1155 0.81 -2.0 1,255,492 + Psest_1155 0.81 +0.1 1,255,492 + Psest_1155 0.81 -2.1 1,255,492 + Psest_1155 0.81 -2.8 1,255,493 - Psest_1155 0.81 +1.1 1,255,493 - Psest_1155 0.81 +1.8 1,255,695 + -0.4 1,255,808 + +0.6 1,255,809 - +0.3 1,255,809 - -0.6 1,255,809 - +1.0 1,255,877 + -1.4 1,255,878 - +0.3 1,255,878 - +1.0 1,255,888 + -1.2 1,255,888 + -1.6 1,255,888 + -0.2 1,255,888 + -0.2 1,255,889 - -1.6 1,255,889 - -1.3 1,255,889 - -2.1 1,255,889 - -1.3 1,256,088 + Psest_1156 0.19 -1.2 1,256,088 + Psest_1156 0.19 +1.3 1,256,151 - Psest_1156 0.25 -0.3 1,256,258 + Psest_1156 0.35 +1.8 1,256,259 - Psest_1156 0.35 -1.9 1,256,601 - Psest_1156 0.66 -1.3 1,256,601 - Psest_1156 0.66 -0.0 1,256,601 - Psest_1156 0.66 +0.1 1,256,601 - Psest_1156 0.66 +0.8 1,256,640 - Psest_1156 0.70 -1.0 1,256,640 - Psest_1156 0.70 -1.7 1,256,705 + Psest_1156 0.76 -2.7 1,256,705 + Psest_1156 0.76 -0.5 1,256,706 - Psest_1156 0.76 -0.7 1,256,706 - Psest_1156 0.76 +3.9 1,256,706 - Psest_1156 0.76 -0.5 1,256,754 - Psest_1156 0.80 -0.8 1,256,754 - Psest_1156 0.80 -3.0
Or see this region's nucleotide sequence