Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0724 and Psest_0725 are separated by 104 nucleotides Psest_0725 and Psest_0726 are separated by 16 nucleotides Psest_0726 and Psest_0727 are separated by 262 nucleotides
Psest_0724: Psest_0724 - Deoxyinosine 3'endonuclease (endonuclease V), at 793,863 to 794,591
_0724
Psest_0725: Psest_0725 - Negative regulator of beta-lactamase expression, at 794,696 to 795,247
_0725
Psest_0726: Psest_0726 - Membrane protein required for beta-lactamase induction, at 795,264 to 796,100
_0726
Psest_0727: Psest_0727 - Methyl-accepting chemotaxis protein, at 796,363 to 798,393
_0727
Position (kb)
795
796
797 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 794.266 kb on + strand, within Psest_0724 at 794.395 kb on + strand, within Psest_0724 at 794.605 kb on + strand at 794.781 kb on + strand, within Psest_0725 at 794.781 kb on + strand, within Psest_0725 at 794.793 kb on + strand, within Psest_0725 at 794.794 kb on - strand, within Psest_0725 at 794.794 kb on - strand, within Psest_0725 at 795.055 kb on + strand, within Psest_0725 at 795.238 kb on + strand at 795.239 kb on - strand at 795.239 kb on - strand at 795.415 kb on + strand, within Psest_0726 at 795.519 kb on + strand, within Psest_0726 at 795.519 kb on + strand, within Psest_0726 at 795.520 kb on - strand, within Psest_0726 at 795.520 kb on - strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.690 kb on + strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.691 kb on - strand, within Psest_0726 at 795.854 kb on - strand, within Psest_0726 at 795.859 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.934 kb on + strand, within Psest_0726 at 795.935 kb on - strand, within Psest_0726 at 795.935 kb on - strand, within Psest_0726 at 796.045 kb on + strand at 796.249 kb on - strand at 796.249 kb on - strand at 796.274 kb on - strand at 796.345 kb on - strand at 796.368 kb on + strand at 796.369 kb on - strand at 796.369 kb on - strand at 796.369 kb on - strand at 796.369 kb on - strand at 796.378 kb on + strand at 796.379 kb on - strand at 796.379 kb on - strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.471 kb on + strand at 796.472 kb on - strand at 796.472 kb on - strand at 796.472 kb on - strand at 796.472 kb on - strand at 796.472 kb on - strand at 796.472 kb on - strand at 796.472 kb on - strand at 796.472 kb on - strand at 796.472 kb on - strand at 796.474 kb on + strand at 796.474 kb on + strand at 796.975 kb on + strand, within Psest_0727 at 796.976 kb on - strand, within Psest_0727 at 796.994 kb on - strand, within Psest_0727 at 796.994 kb on - strand, within Psest_0727 at 797.062 kb on + strand, within Psest_0727
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 794,266 + Psest_0724 0.55 -1.0 794,395 + Psest_0724 0.73 -0.4 794,605 + +4.3 794,781 + Psest_0725 0.15 +0.8 794,781 + Psest_0725 0.15 +2.6 794,793 + Psest_0725 0.18 -2.0 794,794 - Psest_0725 0.18 +2.1 794,794 - Psest_0725 0.18 -2.0 795,055 + Psest_0725 0.65 -0.7 795,238 + +0.0 795,239 - +0.9 795,239 - +2.3 795,415 + Psest_0726 0.18 -3.3 795,519 + Psest_0726 0.30 -2.2 795,519 + Psest_0726 0.30 -1.5 795,520 - Psest_0726 0.31 +1.0 795,520 - Psest_0726 0.31 +0.7 795,690 + Psest_0726 0.51 -2.8 795,690 + Psest_0726 0.51 -0.2 795,690 + Psest_0726 0.51 +0.3 795,690 + Psest_0726 0.51 +1.1 795,690 + Psest_0726 0.51 -0.6 795,690 + Psest_0726 0.51 -0.7 795,690 + Psest_0726 0.51 -2.1 795,690 + Psest_0726 0.51 -0.0 795,690 + Psest_0726 0.51 +0.2 795,690 + Psest_0726 0.51 -0.1 795,690 + Psest_0726 0.51 +0.8 795,691 - Psest_0726 0.51 -1.0 795,691 - Psest_0726 0.51 -1.6 795,691 - Psest_0726 0.51 +0.9 795,691 - Psest_0726 0.51 +1.2 795,691 - Psest_0726 0.51 +0.3 795,691 - Psest_0726 0.51 +0.0 795,691 - Psest_0726 0.51 +2.1 795,854 - Psest_0726 0.70 -1.1 795,859 + Psest_0726 0.71 -0.6 795,934 + Psest_0726 0.80 -1.3 795,934 + Psest_0726 0.80 -2.6 795,934 + Psest_0726 0.80 -1.0 795,934 + Psest_0726 0.80 -1.9 795,934 + Psest_0726 0.80 -1.4 795,935 - Psest_0726 0.80 -2.2 795,935 - Psest_0726 0.80 +0.5 796,045 + +0.3 796,249 - -0.1 796,249 - -0.7 796,274 - -2.0 796,345 - +1.4 796,368 + -0.8 796,369 - -0.7 796,369 - -1.4 796,369 - -0.2 796,369 - -2.1 796,378 + +0.6 796,379 - -1.3 796,379 - +1.2 796,471 + -2.1 796,471 + -0.2 796,471 + -0.3 796,471 + -0.4 796,471 + +0.1 796,471 + +4.7 796,471 + -1.2 796,471 + +0.5 796,471 + +0.1 796,471 + +0.7 796,471 + -0.9 796,472 - +0.5 796,472 - +0.1 796,472 - -1.2 796,472 - +0.5 796,472 - +1.5 796,472 - +3.0 796,472 - -0.8 796,472 - -0.2 796,472 - +0.4 796,474 + -1.1 796,474 + -2.9 796,975 + Psest_0727 0.30 +0.5 796,976 - Psest_0727 0.30 +1.7 796,994 - Psest_0727 0.31 +2.8 796,994 - Psest_0727 0.31 -0.7 797,062 + Psest_0727 0.34 -0.5
Or see this region's nucleotide sequence