Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0052 and Psest_0053 are separated by 167 nucleotides Psest_0053 and Psest_0054 are separated by 86 nucleotides Psest_0054 and Psest_0055 are separated by 211 nucleotides
Psest_0052: Psest_0052 - hypothetical protein, at 54,527 to 55,279
_0052
Psest_0053: Psest_0053 - hypothetical protein, at 55,447 to 55,950
_0053
Psest_0054: Psest_0054 - hypothetical protein, at 56,037 to 56,429
_0054
Psest_0055: Psest_0055 - hypothetical protein, at 56,641 to 57,462
_0055
Position (kb)
56
57 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 55.065 kb on + strand, within Psest_0052 at 55.065 kb on + strand, within Psest_0052 at 55.066 kb on - strand, within Psest_0052 at 55.066 kb on - strand, within Psest_0052 at 55.168 kb on - strand, within Psest_0052 at 55.199 kb on + strand, within Psest_0052 at 55.200 kb on - strand, within Psest_0052 at 55.200 kb on - strand, within Psest_0052 at 55.264 kb on - strand at 55.306 kb on + strand at 55.313 kb on - strand at 55.313 kb on - strand at 55.421 kb on - strand at 55.424 kb on - strand at 55.424 kb on - strand at 55.424 kb on - strand at 55.834 kb on + strand, within Psest_0053 at 56.182 kb on + strand, within Psest_0054 at 56.183 kb on - strand, within Psest_0054 at 56.183 kb on - strand, within Psest_0054 at 56.200 kb on + strand, within Psest_0054 at 56.201 kb on - strand, within Psest_0054 at 56.248 kb on + strand, within Psest_0054 at 56.249 kb on - strand, within Psest_0054 at 56.249 kb on - strand, within Psest_0054 at 56.423 kb on + strand at 56.424 kb on - strand at 56.424 kb on - strand at 56.424 kb on - strand at 56.482 kb on + strand at 56.482 kb on + strand at 56.483 kb on - strand at 56.546 kb on + strand at 56.546 kb on + strand at 56.547 kb on - strand at 56.667 kb on + strand at 56.668 kb on - strand at 56.668 kb on - strand at 56.864 kb on + strand, within Psest_0055 at 56.865 kb on - strand, within Psest_0055 at 56.865 kb on - strand, within Psest_0055 at 56.865 kb on - strand, within Psest_0055 at 57.088 kb on + strand, within Psest_0055 at 57.088 kb on + strand, within Psest_0055 at 57.088 kb on + strand, within Psest_0055 at 57.088 kb on + strand, within Psest_0055 at 57.088 kb on + strand, within Psest_0055 at 57.088 kb on + strand, within Psest_0055 at 57.088 kb on + strand, within Psest_0055 at 57.089 kb on - strand, within Psest_0055 at 57.089 kb on - strand, within Psest_0055 at 57.107 kb on + strand, within Psest_0055 at 57.107 kb on + strand, within Psest_0055 at 57.107 kb on + strand, within Psest_0055 at 57.108 kb on - strand, within Psest_0055 at 57.108 kb on - strand, within Psest_0055 at 57.108 kb on - strand, within Psest_0055 at 57.108 kb on - strand, within Psest_0055 at 57.209 kb on + strand, within Psest_0055 at 57.210 kb on - strand, within Psest_0055 at 57.285 kb on + strand, within Psest_0055 at 57.285 kb on + strand, within Psest_0055 at 57.317 kb on + strand, within Psest_0055 at 57.318 kb on - strand, within Psest_0055 at 57.318 kb on - strand, within Psest_0055 at 57.329 kb on + strand, within Psest_0055
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 55,065 + Psest_0052 0.71 -1.3 55,065 + Psest_0052 0.71 +1.0 55,066 - Psest_0052 0.72 +0.5 55,066 - Psest_0052 0.72 -2.3 55,168 - Psest_0052 0.85 +0.1 55,199 + Psest_0052 0.89 -2.3 55,200 - Psest_0052 0.89 -2.9 55,200 - Psest_0052 0.89 +1.1 55,264 - +1.7 55,306 + +1.1 55,313 - +1.6 55,313 - -2.0 55,421 - -0.5 55,424 - +2.0 55,424 - +0.4 55,424 - -0.7 55,834 + Psest_0053 0.77 -0.9 56,182 + Psest_0054 0.37 +0.2 56,183 - Psest_0054 0.37 -1.4 56,183 - Psest_0054 0.37 +1.7 56,200 + Psest_0054 0.41 -1.5 56,201 - Psest_0054 0.42 -1.1 56,248 + Psest_0054 0.54 -1.4 56,249 - Psest_0054 0.54 +0.5 56,249 - Psest_0054 0.54 -0.7 56,423 + +0.5 56,424 - -0.0 56,424 - -1.2 56,424 - +1.0 56,482 + -1.2 56,482 + +0.8 56,483 - +2.0 56,546 + -1.3 56,546 + -3.0 56,547 - +5.4 56,667 + -1.1 56,668 - -1.3 56,668 - -1.8 56,864 + Psest_0055 0.27 +0.6 56,865 - Psest_0055 0.27 -2.4 56,865 - Psest_0055 0.27 +0.2 56,865 - Psest_0055 0.27 +0.3 57,088 + Psest_0055 0.54 -0.9 57,088 + Psest_0055 0.54 +0.2 57,088 + Psest_0055 0.54 +3.1 57,088 + Psest_0055 0.54 +0.6 57,088 + Psest_0055 0.54 -0.9 57,088 + Psest_0055 0.54 +1.2 57,088 + Psest_0055 0.54 +0.7 57,089 - Psest_0055 0.55 +1.7 57,089 - Psest_0055 0.55 -0.3 57,107 + Psest_0055 0.57 +0.6 57,107 + Psest_0055 0.57 +1.1 57,107 + Psest_0055 0.57 -1.0 57,108 - Psest_0055 0.57 -0.4 57,108 - Psest_0055 0.57 +0.4 57,108 - Psest_0055 0.57 +1.1 57,108 - Psest_0055 0.57 -0.8 57,209 + Psest_0055 0.69 +1.0 57,210 - Psest_0055 0.69 +1.4 57,285 + Psest_0055 0.78 -2.7 57,285 + Psest_0055 0.78 -0.4 57,317 + Psest_0055 0.82 -2.3 57,318 - Psest_0055 0.82 -3.0 57,318 - Psest_0055 0.82 -0.7 57,329 + Psest_0055 0.84 -1.7
Or see this region's nucleotide sequence