Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2933 and Psest_2934 are separated by 44 nucleotides Psest_2934 and Psest_2935 are separated by 12 nucleotides Psest_2935 and Psest_2936 are separated by 110 nucleotides Psest_2936 and Psest_2937 are separated by 101 nucleotides
Psest_2933: Psest_2933 - Signal transduction histidine kinase, at 3,129,820 to 3,132,564
_2933
Psest_2934: Psest_2934 - Uncharacterized protein conserved in bacteria, at 3,132,609 to 3,132,836
_2934
Psest_2935: Psest_2935 - hypothetical protein, at 3,132,849 to 3,133,277
_2935
Psest_2936: Psest_2936 - Cyanate permease, at 3,133,388 to 3,134,545
_2936
Psest_2937: Psest_2937 - Short-chain alcohol dehydrogenase of unknown specificity, at 3,134,647 to 3,135,456
_2937
Position (kb)
3133
3134
3135 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3132.523 kb on + strand at 3132.523 kb on + strand at 3132.541 kb on + strand at 3132.541 kb on + strand at 3132.541 kb on + strand at 3132.541 kb on + strand at 3132.562 kb on + strand at 3132.562 kb on + strand at 3133.048 kb on + strand, within Psest_2935 at 3133.048 kb on + strand, within Psest_2935 at 3133.256 kb on - strand at 3133.318 kb on + strand at 3133.318 kb on + strand at 3133.319 kb on - strand at 3133.319 kb on - strand at 3133.472 kb on - strand at 3133.472 kb on - strand at 3133.472 kb on - strand at 3133.472 kb on - strand at 3133.472 kb on - strand at 3133.596 kb on + strand, within Psest_2936 at 3133.596 kb on + strand, within Psest_2936 at 3133.597 kb on - strand, within Psest_2936 at 3133.597 kb on - strand, within Psest_2936 at 3133.706 kb on + strand, within Psest_2936 at 3133.706 kb on + strand, within Psest_2936 at 3133.707 kb on - strand, within Psest_2936 at 3133.891 kb on - strand, within Psest_2936 at 3133.891 kb on - strand, within Psest_2936 at 3133.891 kb on - strand, within Psest_2936 at 3133.891 kb on - strand, within Psest_2936 at 3133.917 kb on + strand, within Psest_2936 at 3133.917 kb on + strand, within Psest_2936 at 3133.918 kb on - strand, within Psest_2936 at 3133.918 kb on - strand, within Psest_2936 at 3134.103 kb on - strand, within Psest_2936 at 3134.175 kb on + strand, within Psest_2936 at 3134.175 kb on + strand, within Psest_2936 at 3134.176 kb on - strand, within Psest_2936 at 3134.176 kb on - strand, within Psest_2936 at 3134.474 kb on - strand at 3134.563 kb on - strand at 3134.591 kb on + strand at 3134.702 kb on + strand at 3134.702 kb on + strand at 3134.801 kb on + strand, within Psest_2937 at 3134.801 kb on + strand, within Psest_2937 at 3134.802 kb on - strand, within Psest_2937 at 3134.802 kb on - strand, within Psest_2937 at 3135.008 kb on + strand, within Psest_2937 at 3135.009 kb on - strand, within Psest_2937 at 3135.269 kb on + strand, within Psest_2937 at 3135.269 kb on + strand, within Psest_2937 at 3135.270 kb on - strand, within Psest_2937 at 3135.270 kb on - strand, within Psest_2937 at 3135.450 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 3,132,523 + -0.1 3,132,523 + +0.9 3,132,541 + +1.3 3,132,541 + -0.9 3,132,541 + -0.0 3,132,541 + +2.7 3,132,562 + -0.1 3,132,562 + -0.7 3,133,048 + Psest_2935 0.46 -0.3 3,133,048 + Psest_2935 0.46 +0.1 3,133,256 - -0.8 3,133,318 + -3.1 3,133,318 + -0.2 3,133,319 - -0.1 3,133,319 - +0.1 3,133,472 - +0.9 3,133,472 - -1.2 3,133,472 - +2.2 3,133,472 - -0.8 3,133,472 - -0.4 3,133,596 + Psest_2936 0.18 +0.1 3,133,596 + Psest_2936 0.18 -0.1 3,133,597 - Psest_2936 0.18 +1.0 3,133,597 - Psest_2936 0.18 -0.1 3,133,706 + Psest_2936 0.27 +0.9 3,133,706 + Psest_2936 0.27 -0.4 3,133,707 - Psest_2936 0.28 -0.1 3,133,891 - Psest_2936 0.43 +1.3 3,133,891 - Psest_2936 0.43 -2.1 3,133,891 - Psest_2936 0.43 +1.7 3,133,891 - Psest_2936 0.43 -2.6 3,133,917 + Psest_2936 0.46 -0.2 3,133,917 + Psest_2936 0.46 +1.1 3,133,918 - Psest_2936 0.46 +0.1 3,133,918 - Psest_2936 0.46 +0.2 3,134,103 - Psest_2936 0.62 +1.0 3,134,175 + Psest_2936 0.68 -0.1 3,134,175 + Psest_2936 0.68 +0.4 3,134,176 - Psest_2936 0.68 -1.3 3,134,176 - Psest_2936 0.68 -1.4 3,134,474 - -0.2 3,134,563 - +0.7 3,134,591 + -1.5 3,134,702 + -0.5 3,134,702 + +1.4 3,134,801 + Psest_2937 0.19 -1.9 3,134,801 + Psest_2937 0.19 +1.2 3,134,802 - Psest_2937 0.19 +2.1 3,134,802 - Psest_2937 0.19 +0.2 3,135,008 + Psest_2937 0.45 +2.4 3,135,009 - Psest_2937 0.45 +0.3 3,135,269 + Psest_2937 0.77 +1.4 3,135,269 + Psest_2937 0.77 +0.5 3,135,270 - Psest_2937 0.77 -0.1 3,135,270 - Psest_2937 0.77 -0.1 3,135,450 + -1.5
Or see this region's nucleotide sequence