Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2497 and Psest_2498 overlap by 7 nucleotides Psest_2498 and Psest_2499 are separated by 361 nucleotides Psest_2499 and Psest_2500 are separated by 203 nucleotides Psest_2500 and Psest_2501 are separated by 129 nucleotides
Psest_2497: Psest_2497 - succinate dehydrogenase, hydrophobic membrane anchor protein, at 2,673,892 to 2,674,260
_2497
Psest_2498: Psest_2498 - succinate dehydrogenase, cytochrome b556 subunit, at 2,674,254 to 2,674,628
_2498
Psest_2499: Psest_2499 - citrate synthase I (hexameric type), at 2,674,990 to 2,676,261
_2499
Psest_2500: Psest_2500 - hypothetical protein, at 2,676,465 to 2,676,716
_2500
Psest_2501: Psest_2501 - Uncharacterized protein conserved in bacteria, at 2,676,846 to 2,677,187
_2501
Position (kb)
2674
2675
2676
2677 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2674.728 kb on - strand at 2674.764 kb on - strand at 2674.766 kb on - strand at 2674.766 kb on - strand at 2674.774 kb on - strand at 2674.774 kb on - strand at 2674.774 kb on - strand at 2674.774 kb on - strand at 2674.827 kb on - strand at 2674.827 kb on - strand at 2674.827 kb on - strand at 2674.827 kb on - strand at 2674.827 kb on - strand at 2674.827 kb on - strand at 2674.827 kb on - strand at 2674.827 kb on - strand at 2674.829 kb on - strand at 2674.829 kb on - strand at 2674.829 kb on - strand at 2674.829 kb on - strand at 2674.830 kb on + strand at 2674.830 kb on + strand at 2674.830 kb on + strand at 2674.830 kb on + strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.831 kb on - strand at 2674.860 kb on - strand at 2674.860 kb on - strand at 2674.860 kb on - strand at 2674.860 kb on - strand at 2674.884 kb on - strand at 2674.918 kb on + strand at 2674.918 kb on + strand at 2674.918 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.926 kb on + strand at 2674.927 kb on - strand at 2674.927 kb on - strand at 2674.928 kb on + strand at 2674.928 kb on + strand at 2675.078 kb on + strand at 2675.078 kb on + strand at 2675.079 kb on - strand at 2675.164 kb on + strand, within Psest_2499 at 2675.237 kb on - strand, within Psest_2499 at 2675.915 kb on - strand, within Psest_2499 at 2676.227 kb on - strand at 2676.404 kb on - strand at 2676.430 kb on + strand at 2676.558 kb on + strand, within Psest_2500 at 2676.558 kb on + strand, within Psest_2500 at 2676.594 kb on + strand, within Psest_2500 at 2676.594 kb on + strand, within Psest_2500 at 2676.595 kb on - strand, within Psest_2500 at 2676.749 kb on + strand at 2676.752 kb on + strand at 2676.753 kb on - strand at 2676.809 kb on - strand at 2676.809 kb on - strand at 2676.809 kb on - strand at 2676.809 kb on - strand at 2676.913 kb on - strand, within Psest_2501 at 2676.922 kb on + strand, within Psest_2501 at 2676.922 kb on + strand, within Psest_2501 at 2676.922 kb on + strand, within Psest_2501 at 2676.922 kb on + strand, within Psest_2501 at 2676.923 kb on - strand, within Psest_2501 at 2676.923 kb on - strand, within Psest_2501 at 2676.990 kb on + strand, within Psest_2501 at 2676.990 kb on + strand, within Psest_2501 at 2676.990 kb on + strand, within Psest_2501 at 2676.990 kb on + strand, within Psest_2501 at 2676.991 kb on - strand, within Psest_2501 at 2676.991 kb on - strand, within Psest_2501 at 2676.991 kb on - strand, within Psest_2501 at 2677.024 kb on - strand, within Psest_2501 at 2677.033 kb on + strand, within Psest_2501 at 2677.034 kb on - strand, within Psest_2501 at 2677.066 kb on + strand, within Psest_2501 at 2677.066 kb on + strand, within Psest_2501 at 2677.066 kb on + strand, within Psest_2501 at 2677.066 kb on + strand, within Psest_2501 at 2677.067 kb on - strand, within Psest_2501 at 2677.067 kb on - strand, within Psest_2501 at 2677.067 kb on - strand, within Psest_2501 at 2677.104 kb on + strand, within Psest_2501 at 2677.104 kb on + strand, within Psest_2501 at 2677.104 kb on + strand, within Psest_2501 at 2677.104 kb on + strand, within Psest_2501 at 2677.105 kb on - strand, within Psest_2501 at 2677.105 kb on - strand, within Psest_2501 at 2677.105 kb on - strand, within Psest_2501 at 2677.105 kb on - strand, within Psest_2501 at 2677.105 kb on - strand, within Psest_2501 at 2677.105 kb on - strand, within Psest_2501 at 2677.131 kb on + strand, within Psest_2501 at 2677.132 kb on - strand, within Psest_2501 at 2677.132 kb on - strand, within Psest_2501 at 2677.132 kb on - strand, within Psest_2501 at 2677.132 kb on - strand, within Psest_2501 at 2677.132 kb on - strand, within Psest_2501 at 2677.132 kb on - strand, within Psest_2501 at 2677.167 kb on + strand at 2677.167 kb on + strand at 2677.167 kb on + strand at 2677.167 kb on + strand at 2677.167 kb on + strand at 2677.167 kb on + strand at 2677.167 kb on + strand at 2677.167 kb on + strand at 2677.167 kb on + strand at 2677.168 kb on - strand at 2677.168 kb on - strand at 2677.168 kb on - strand at 2677.168 kb on - strand at 2677.168 kb on - strand at 2677.168 kb on - strand at 2677.168 kb on - strand at 2677.168 kb on - strand at 2677.172 kb on + strand at 2677.172 kb on + strand at 2677.172 kb on + strand at 2677.173 kb on - strand at 2677.185 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 2,674,728 - +0.7 2,674,764 - -1.0 2,674,766 - +0.1 2,674,766 - -2.5 2,674,774 - -1.6 2,674,774 - -0.0 2,674,774 - -0.3 2,674,774 - -2.5 2,674,827 - -1.4 2,674,827 - +1.9 2,674,827 - +0.2 2,674,827 - -0.7 2,674,827 - -1.7 2,674,827 - +1.7 2,674,827 - +1.5 2,674,827 - +0.3 2,674,829 - -2.3 2,674,829 - -0.4 2,674,829 - -2.1 2,674,829 - -1.6 2,674,830 + +1.4 2,674,830 + -0.6 2,674,830 + +0.4 2,674,830 + -0.2 2,674,831 - -0.4 2,674,831 - -2.4 2,674,831 - +1.0 2,674,831 - -1.8 2,674,831 - -1.1 2,674,831 - +2.0 2,674,831 - -1.0 2,674,831 - -0.2 2,674,831 - -0.3 2,674,831 - -3.3 2,674,831 - -2.0 2,674,831 - -0.3 2,674,831 - +0.2 2,674,831 - -2.4 2,674,831 - +0.6 2,674,831 - -0.3 2,674,860 - +0.4 2,674,860 - -0.7 2,674,860 - -2.9 2,674,860 - -0.2 2,674,884 - +2.9 2,674,918 + -0.2 2,674,918 + -1.9 2,674,918 + -0.9 2,674,926 + -0.9 2,674,926 + -0.6 2,674,926 + -0.9 2,674,926 + -1.8 2,674,926 + -0.9 2,674,926 + +0.9 2,674,926 + -1.4 2,674,926 + -0.2 2,674,926 + -1.2 2,674,926 + -1.3 2,674,927 - -0.4 2,674,927 - +2.1 2,674,928 + +2.4 2,674,928 + -1.6 2,675,078 + -1.2 2,675,078 + -1.2 2,675,079 - +0.4 2,675,164 + Psest_2499 0.14 -0.9 2,675,237 - Psest_2499 0.19 -0.2 2,675,915 - Psest_2499 0.73 -0.9 2,676,227 - +1.4 2,676,404 - -0.0 2,676,430 + +3.3 2,676,558 + Psest_2500 0.37 -2.9 2,676,558 + Psest_2500 0.37 -0.4 2,676,594 + Psest_2500 0.51 -2.1 2,676,594 + Psest_2500 0.51 -2.3 2,676,595 - Psest_2500 0.52 +0.8 2,676,749 + -1.4 2,676,752 + -0.4 2,676,753 - +0.4 2,676,809 - +2.2 2,676,809 - -0.9 2,676,809 - -0.4 2,676,809 - -1.1 2,676,913 - Psest_2501 0.20 +1.1 2,676,922 + Psest_2501 0.22 -0.6 2,676,922 + Psest_2501 0.22 -1.4 2,676,922 + Psest_2501 0.22 +2.4 2,676,922 + Psest_2501 0.22 +1.2 2,676,923 - Psest_2501 0.23 +0.4 2,676,923 - Psest_2501 0.23 +1.3 2,676,990 + Psest_2501 0.42 +0.4 2,676,990 + Psest_2501 0.42 +0.4 2,676,990 + Psest_2501 0.42 +2.3 2,676,990 + Psest_2501 0.42 +0.1 2,676,991 - Psest_2501 0.42 +0.1 2,676,991 - Psest_2501 0.42 +2.1 2,676,991 - Psest_2501 0.42 +0.6 2,677,024 - Psest_2501 0.52 +0.0 2,677,033 + Psest_2501 0.55 -1.0 2,677,034 - Psest_2501 0.55 +1.7 2,677,066 + Psest_2501 0.64 +0.6 2,677,066 + Psest_2501 0.64 +1.9 2,677,066 + Psest_2501 0.64 +1.0 2,677,066 + Psest_2501 0.64 -0.4 2,677,067 - Psest_2501 0.65 +1.0 2,677,067 - Psest_2501 0.65 -1.0 2,677,067 - Psest_2501 0.65 -1.4 2,677,104 + Psest_2501 0.75 -0.9 2,677,104 + Psest_2501 0.75 -2.0 2,677,104 + Psest_2501 0.75 +0.5 2,677,104 + Psest_2501 0.75 +0.4 2,677,105 - Psest_2501 0.76 +0.6 2,677,105 - Psest_2501 0.76 -0.1 2,677,105 - Psest_2501 0.76 +0.4 2,677,105 - Psest_2501 0.76 -0.9 2,677,105 - Psest_2501 0.76 -0.1 2,677,105 - Psest_2501 0.76 -0.2 2,677,131 + Psest_2501 0.83 -0.3 2,677,132 - Psest_2501 0.84 -0.0 2,677,132 - Psest_2501 0.84 +2.9 2,677,132 - Psest_2501 0.84 -1.2 2,677,132 - Psest_2501 0.84 -3.1 2,677,132 - Psest_2501 0.84 +1.3 2,677,132 - Psest_2501 0.84 -1.1 2,677,167 + +0.4 2,677,167 + -0.0 2,677,167 + +0.1 2,677,167 + +1.4 2,677,167 + -0.3 2,677,167 + +0.2 2,677,167 + -1.0 2,677,167 + +0.5 2,677,167 + -0.1 2,677,168 - +0.4 2,677,168 - +1.4 2,677,168 - +0.7 2,677,168 - +0.6 2,677,168 - -1.9 2,677,168 - -1.8 2,677,168 - +1.0 2,677,168 - +1.1 2,677,172 + -0.0 2,677,172 + +1.4 2,677,172 + -2.1 2,677,173 - -0.9 2,677,185 + -2.1
Or see this region's nucleotide sequence