Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2437 and Psest_2438 are separated by 36 nucleotides Psest_2438 and Psest_2439 are separated by 2 nucleotides Psest_2439 and Psest_2440 are separated by 38 nucleotides
Psest_2437: Psest_2437 - Enoyl-CoA hydratase [valine degradation] (EC 4.2.1.17) (from data) , at 2,605,492 to 2,606,265
_2437
Psest_2438: Psest_2438 - 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) (from data) , at 2,606,302 to 2,607,192
_2438
Psest_2439: Psest_2439 - 3-hydroxyisobutyryl-CoA hydrolase (EC 3.1.2.4) (from data) , at 2,607,195 to 2,608,289
_2439
Psest_2440: Psest_2440 - isobutyryl-CoA dehydrogenase (EC 1.3.8.5) (from data) , at 2,608,328 to 2,609,479
_2440
Position (kb)
2607
2608
2609 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2606.280 kb on + strand at 2606.316 kb on + strand at 2606.317 kb on - strand at 2606.524 kb on + strand, within Psest_2438 at 2606.524 kb on + strand, within Psest_2438 at 2606.524 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.956 kb on + strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2606.957 kb on - strand, within Psest_2438 at 2607.004 kb on + strand, within Psest_2438 at 2607.230 kb on - strand at 2607.468 kb on + strand, within Psest_2439 at 2607.469 kb on - strand, within Psest_2439 at 2607.483 kb on + strand, within Psest_2439 at 2607.483 kb on + strand, within Psest_2439 at 2607.484 kb on - strand, within Psest_2439 at 2607.602 kb on + strand, within Psest_2439 at 2607.625 kb on - strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.648 kb on + strand, within Psest_2439 at 2607.649 kb on - strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.684 kb on + strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2607.685 kb on - strand, within Psest_2439 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.517 kb on + strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.518 kb on - strand, within Psest_2440 at 2608.520 kb on - strand, within Psest_2440 at 2608.526 kb on + strand, within Psest_2440 at 2608.527 kb on - strand, within Psest_2440 at 2608.527 kb on - strand, within Psest_2440 at 2608.632 kb on - strand, within Psest_2440 at 2609.120 kb on + strand, within Psest_2440
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 2,606,280 + -0.4 2,606,316 + +0.5 2,606,317 - -2.2 2,606,524 + Psest_2438 0.25 -0.9 2,606,524 + Psest_2438 0.25 -2.3 2,606,524 + Psest_2438 0.25 -0.3 2,606,956 + Psest_2438 0.73 -1.4 2,606,956 + Psest_2438 0.73 -4.0 2,606,956 + Psest_2438 0.73 -1.1 2,606,956 + Psest_2438 0.73 -1.9 2,606,956 + Psest_2438 0.73 -0.2 2,606,956 + Psest_2438 0.73 -1.6 2,606,956 + Psest_2438 0.73 +0.2 2,606,957 - Psest_2438 0.74 -1.3 2,606,957 - Psest_2438 0.74 +1.0 2,606,957 - Psest_2438 0.74 +0.3 2,606,957 - Psest_2438 0.74 +0.3 2,606,957 - Psest_2438 0.74 -1.4 2,606,957 - Psest_2438 0.74 -1.4 2,606,957 - Psest_2438 0.74 -3.5 2,606,957 - Psest_2438 0.74 +0.2 2,606,957 - Psest_2438 0.74 -1.6 2,606,957 - Psest_2438 0.74 -2.4 2,606,957 - Psest_2438 0.74 -2.6 2,606,957 - Psest_2438 0.74 -0.2 2,606,957 - Psest_2438 0.74 -1.9 2,606,957 - Psest_2438 0.74 +0.6 2,606,957 - Psest_2438 0.74 -0.9 2,607,004 + Psest_2438 0.79 -1.0 2,607,230 - +0.1 2,607,468 + Psest_2439 0.25 -1.6 2,607,469 - Psest_2439 0.25 +0.5 2,607,483 + Psest_2439 0.26 -0.6 2,607,483 + Psest_2439 0.26 -2.1 2,607,484 - Psest_2439 0.26 -2.2 2,607,602 + Psest_2439 0.37 -1.0 2,607,625 - Psest_2439 0.39 -0.8 2,607,648 + Psest_2439 0.41 -2.1 2,607,648 + Psest_2439 0.41 -0.8 2,607,648 + Psest_2439 0.41 -2.3 2,607,649 - Psest_2439 0.41 -2.1 2,607,684 + Psest_2439 0.45 -2.2 2,607,684 + Psest_2439 0.45 +0.7 2,607,684 + Psest_2439 0.45 +0.1 2,607,684 + Psest_2439 0.45 -0.2 2,607,684 + Psest_2439 0.45 -2.4 2,607,684 + Psest_2439 0.45 -0.7 2,607,684 + Psest_2439 0.45 +0.5 2,607,684 + Psest_2439 0.45 +1.8 2,607,685 - Psest_2439 0.45 -1.5 2,607,685 - Psest_2439 0.45 +1.1 2,607,685 - Psest_2439 0.45 +1.2 2,607,685 - Psest_2439 0.45 -0.7 2,607,685 - Psest_2439 0.45 -1.0 2,607,685 - Psest_2439 0.45 -0.7 2,607,685 - Psest_2439 0.45 -1.9 2,607,685 - Psest_2439 0.45 +0.2 2,607,685 - Psest_2439 0.45 -0.9 2,607,685 - Psest_2439 0.45 -0.4 2,607,685 - Psest_2439 0.45 -0.0 2,607,685 - Psest_2439 0.45 -0.6 2,607,685 - Psest_2439 0.45 -1.1 2,607,685 - Psest_2439 0.45 -1.3 2,608,517 + Psest_2440 0.16 -0.9 2,608,517 + Psest_2440 0.16 +0.1 2,608,517 + Psest_2440 0.16 -1.9 2,608,517 + Psest_2440 0.16 +1.1 2,608,517 + Psest_2440 0.16 -1.4 2,608,517 + Psest_2440 0.16 -0.9 2,608,517 + Psest_2440 0.16 +0.3 2,608,517 + Psest_2440 0.16 +0.1 2,608,517 + Psest_2440 0.16 -0.6 2,608,518 - Psest_2440 0.16 -0.7 2,608,518 - Psest_2440 0.16 -1.2 2,608,518 - Psest_2440 0.16 +0.8 2,608,518 - Psest_2440 0.16 +0.6 2,608,518 - Psest_2440 0.16 +1.4 2,608,518 - Psest_2440 0.16 -1.4 2,608,520 - Psest_2440 0.17 -0.2 2,608,526 + Psest_2440 0.17 -3.3 2,608,527 - Psest_2440 0.17 -0.9 2,608,527 - Psest_2440 0.17 +0.6 2,608,632 - Psest_2440 0.26 +0.3 2,609,120 + Psest_2440 0.69 -0.9
Or see this region's nucleotide sequence