Experiment: RCH2 grown on a colony of ecoli
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0202 and Psest_0203 overlap by 20 nucleotides Psest_0203 and Psest_0204 overlap by 8 nucleotides Psest_0204 and Psest_0205 are separated by 182 nucleotides
Psest_0202: Psest_0202 - diguanylate cyclase (GGDEF) domain, at 200,892 to 202,823
_0202
Psest_0203: Psest_0203 - Signal transduction histidine kinase, at 202,804 to 204,204
_0203
Psest_0204: Psest_0204 - Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain, at 204,197 to 204,868
_0204
Psest_0205: Psest_0205 - tricarboxylate porin <small>(from data)</small>, at 205,051 to 206,313
_0205
Position (kb) 202
203
204
205 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 202.209 kb on + strand, within Psest_0202 at 202.209 kb on + strand, within Psest_0202 at 202.348 kb on + strand, within Psest_0202 at 202.728 kb on + strand at 202.728 kb on + strand at 202.728 kb on + strand at 202.728 kb on + strand at 202.729 kb on - strand at 202.729 kb on - strand at 202.953 kb on - strand, within Psest_0203 at 202.953 kb on - strand, within Psest_0203 at 203.068 kb on - strand, within Psest_0203 at 203.831 kb on + strand, within Psest_0203 at 203.831 kb on + strand, within Psest_0203 at 203.831 kb on + strand, within Psest_0203 at 203.831 kb on + strand, within Psest_0203 at 203.831 kb on + strand, within Psest_0203 at 203.832 kb on - strand, within Psest_0203 at 203.832 kb on - strand, within Psest_0203 at 203.832 kb on - strand, within Psest_0203 at 203.832 kb on - strand, within Psest_0203 at 203.939 kb on + strand, within Psest_0203 at 203.939 kb on + strand, within Psest_0203 at 203.940 kb on - strand, within Psest_0203 at 203.942 kb on + strand, within Psest_0203 at 203.942 kb on + strand, within Psest_0203 at 203.942 kb on + strand, within Psest_0203 at 203.942 kb on + strand, within Psest_0203 at 203.942 kb on + strand, within Psest_0203 at 203.942 kb on + strand, within Psest_0203 at 203.942 kb on + strand, within Psest_0203 at 203.942 kb on + strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.943 kb on - strand, within Psest_0203 at 203.987 kb on + strand, within Psest_0203 at 203.987 kb on + strand, within Psest_0203 at 203.988 kb on - strand, within Psest_0203 at 204.059 kb on - strand, within Psest_0203 at 204.223 kb on - strand at 204.242 kb on - strand at 204.291 kb on + strand, within Psest_0204 at 204.291 kb on + strand, within Psest_0204 at 204.291 kb on + strand, within Psest_0204 at 204.291 kb on + strand, within Psest_0204 at 204.291 kb on + strand, within Psest_0204 at 204.291 kb on + strand, within Psest_0204 at 204.291 kb on + strand, within Psest_0204 at 204.291 kb on + strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.292 kb on - strand, within Psest_0204 at 204.336 kb on - strand, within Psest_0204 at 204.468 kb on + strand, within Psest_0204 at 204.468 kb on + strand, within Psest_0204 at 204.469 kb on - strand, within Psest_0204 at 204.469 kb on - strand, within Psest_0204 at 204.469 kb on - strand, within Psest_0204 at 204.577 kb on - strand, within Psest_0204 at 204.736 kb on - strand, within Psest_0204 at 204.970 kb on + strand at 204.970 kb on + strand at 205.036 kb on - strand at 205.183 kb on + strand, within Psest_0205 at 205.183 kb on + strand, within Psest_0205 at 205.183 kb on + strand, within Psest_0205 at 205.184 kb on - strand, within Psest_0205 at 205.184 kb on - strand, within Psest_0205
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2 grown on a colony of ecoli remove 202,209 + Psest_0202 0.68 +0.5 202,209 + Psest_0202 0.68 -1.7 202,348 + Psest_0202 0.75 +0.1 202,728 + +1.0 202,728 + -1.0 202,728 + -0.3 202,728 + +2.9 202,729 - +1.7 202,729 - +2.3 202,953 - Psest_0203 0.11 +0.8 202,953 - Psest_0203 0.11 +1.2 203,068 - Psest_0203 0.19 +0.4 203,831 + Psest_0203 0.73 -1.3 203,831 + Psest_0203 0.73 -1.0 203,831 + Psest_0203 0.73 -0.8 203,831 + Psest_0203 0.73 -1.3 203,831 + Psest_0203 0.73 -0.8 203,832 - Psest_0203 0.73 -2.6 203,832 - Psest_0203 0.73 -2.5 203,832 - Psest_0203 0.73 +0.0 203,832 - Psest_0203 0.73 -1.6 203,939 + Psest_0203 0.81 -0.2 203,939 + Psest_0203 0.81 +0.7 203,940 - Psest_0203 0.81 +0.5 203,942 + Psest_0203 0.81 -0.0 203,942 + Psest_0203 0.81 -0.7 203,942 + Psest_0203 0.81 +1.9 203,942 + Psest_0203 0.81 -0.2 203,942 + Psest_0203 0.81 -1.1 203,942 + Psest_0203 0.81 -2.6 203,942 + Psest_0203 0.81 +1.9 203,942 + Psest_0203 0.81 -2.0 203,943 - Psest_0203 0.81 -2.1 203,943 - Psest_0203 0.81 +2.4 203,943 - Psest_0203 0.81 -2.1 203,943 - Psest_0203 0.81 -1.9 203,943 - Psest_0203 0.81 -0.1 203,943 - Psest_0203 0.81 +0.5 203,943 - Psest_0203 0.81 -1.1 203,943 - Psest_0203 0.81 -0.3 203,943 - Psest_0203 0.81 +0.4 203,943 - Psest_0203 0.81 -1.3 203,943 - Psest_0203 0.81 -0.4 203,987 + Psest_0203 0.84 -0.9 203,987 + Psest_0203 0.84 -3.2 203,988 - Psest_0203 0.85 -0.9 204,059 - Psest_0203 0.90 -0.2 204,223 - +0.6 204,242 - +1.1 204,291 + Psest_0204 0.14 -1.9 204,291 + Psest_0204 0.14 -0.2 204,291 + Psest_0204 0.14 -3.1 204,291 + Psest_0204 0.14 +0.8 204,291 + Psest_0204 0.14 +0.5 204,291 + Psest_0204 0.14 +1.2 204,291 + Psest_0204 0.14 +1.7 204,291 + Psest_0204 0.14 -2.0 204,292 - Psest_0204 0.14 -0.9 204,292 - Psest_0204 0.14 +1.1 204,292 - Psest_0204 0.14 -4.0 204,292 - Psest_0204 0.14 +2.3 204,292 - Psest_0204 0.14 +0.5 204,292 - Psest_0204 0.14 -2.2 204,292 - Psest_0204 0.14 +1.4 204,292 - Psest_0204 0.14 -0.8 204,292 - Psest_0204 0.14 +0.5 204,292 - Psest_0204 0.14 -0.3 204,336 - Psest_0204 0.21 +0.4 204,468 + Psest_0204 0.40 -0.5 204,468 + Psest_0204 0.40 +0.4 204,469 - Psest_0204 0.40 +0.6 204,469 - Psest_0204 0.40 -0.3 204,469 - Psest_0204 0.40 -0.2 204,577 - Psest_0204 0.57 +0.0 204,736 - Psest_0204 0.80 +0.3 204,970 + +2.1 204,970 + +0.2 205,036 - +1.7 205,183 + Psest_0205 0.10 +2.7 205,183 + Psest_0205 0.10 -2.0 205,183 + Psest_0205 0.10 -0.3 205,184 - Psest_0205 0.11 -0.9 205,184 - Psest_0205 0.11 +0.1
Or see this region's nucleotide sequence