Experiment: Nalidixic 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0029 and Psest_0030 overlap by 4 nucleotides Psest_0030 and Psest_0031 are separated by 12 nucleotides Psest_0031 and Psest_0032 are separated by 246 nucleotides
Psest_0029: Psest_0029 - shikimate 5-dehydrogenase, at 29,744 to 30,556
_0029
Psest_0030: Psest_0030 - Putative NADH-flavin reductase, at 30,553 to 31,164
_0030
Psest_0031: Psest_0031 - Uncharacterized conserved protein, at 31,177 to 31,485
_0031
Psest_0032: Psest_0032 - D-alanyl-D-alanine carboxypeptidase, at 31,732 to 32,670
_0032
Position (kb)
31
32 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 30.550 kb on + strand at 30.550 kb on + strand at 30.551 kb on - strand at 30.551 kb on - strand at 30.554 kb on + strand at 30.625 kb on - strand, within Psest_0030 at 30.869 kb on + strand, within Psest_0030 at 30.870 kb on - strand, within Psest_0030 at 30.974 kb on - strand, within Psest_0030 at 31.125 kb on - strand at 31.125 kb on - strand at 31.310 kb on + strand, within Psest_0031 at 31.311 kb on - strand, within Psest_0031 at 31.570 kb on + strand at 31.570 kb on + strand at 31.570 kb on + strand at 31.570 kb on + strand at 31.571 kb on - strand at 31.571 kb on - strand at 31.571 kb on - strand at 31.663 kb on - strand at 31.665 kb on - strand at 31.665 kb on - strand at 31.680 kb on - strand at 31.680 kb on - strand at 31.729 kb on - strand at 31.747 kb on - strand at 31.863 kb on + strand, within Psest_0032 at 31.864 kb on - strand, within Psest_0032 at 31.879 kb on + strand, within Psest_0032 at 31.879 kb on + strand, within Psest_0032 at 31.879 kb on + strand, within Psest_0032 at 31.880 kb on - strand, within Psest_0032 at 31.880 kb on - strand, within Psest_0032 at 31.880 kb on - strand, within Psest_0032 at 32.194 kb on + strand, within Psest_0032 at 32.194 kb on + strand, within Psest_0032 at 32.194 kb on + strand, within Psest_0032 at 32.194 kb on + strand, within Psest_0032 at 32.194 kb on + strand, within Psest_0032 at 32.194 kb on + strand, within Psest_0032 at 32.195 kb on - strand, within Psest_0032 at 32.195 kb on - strand, within Psest_0032 at 32.195 kb on - strand, within Psest_0032 at 32.195 kb on - strand, within Psest_0032 at 32.195 kb on - strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.197 kb on + strand, within Psest_0032 at 32.198 kb on - strand, within Psest_0032 at 32.198 kb on - strand, within Psest_0032 at 32.198 kb on - strand, within Psest_0032 at 32.198 kb on - strand, within Psest_0032 at 32.198 kb on - strand, within Psest_0032 at 32.198 kb on - strand, within Psest_0032 at 32.198 kb on - strand, within Psest_0032 at 32.279 kb on - strand, within Psest_0032 at 32.301 kb on + strand, within Psest_0032 at 32.341 kb on + strand, within Psest_0032 at 32.341 kb on + strand, within Psest_0032 at 32.341 kb on + strand, within Psest_0032 at 32.341 kb on + strand, within Psest_0032 at 32.341 kb on + strand, within Psest_0032 at 32.341 kb on + strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand at 32.342 kb on - strand, within Psest_0032 at 32.342 kb on - strand, within Psest_0032 at 32.454 kb on + strand, within Psest_0032 at 32.454 kb on + strand, within Psest_0032 at 32.455 kb on - strand, within Psest_0032 at 32.455 kb on - strand, within Psest_0032
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.00390625 mg/ml remove 30,550 + -0.2 30,550 + +0.4 30,551 - -0.8 30,551 - +0.7 30,554 + -0.0 30,625 - Psest_0030 0.12 +0.0 30,869 + Psest_0030 0.52 -2.0 30,870 - Psest_0030 0.52 -2.8 30,974 - Psest_0030 0.69 +1.5 31,125 - +0.8 31,125 - -0.4 31,310 + Psest_0031 0.43 +1.9 31,311 - Psest_0031 0.43 +1.2 31,570 + -0.8 31,570 + -2.0 31,570 + +0.6 31,570 + -0.2 31,571 - +1.0 31,571 - -2.1 31,571 - -1.5 31,663 - +0.2 31,665 - -0.5 31,665 - -0.1 31,680 - -2.0 31,680 - -2.2 31,729 - -0.5 31,747 - +0.8 31,863 + Psest_0032 0.14 -1.4 31,864 - Psest_0032 0.14 -1.8 31,879 + Psest_0032 0.16 +0.0 31,879 + Psest_0032 0.16 +0.3 31,879 + Psest_0032 0.16 +0.2 31,880 - Psest_0032 0.16 +0.7 31,880 - Psest_0032 0.16 -3.4 31,880 - Psest_0032 0.16 +0.0 32,194 + Psest_0032 0.49 -0.7 32,194 + Psest_0032 0.49 -0.5 32,194 + Psest_0032 0.49 -1.4 32,194 + Psest_0032 0.49 -0.6 32,194 + Psest_0032 0.49 +1.6 32,194 + Psest_0032 0.49 -1.6 32,195 - Psest_0032 0.49 +0.4 32,195 - Psest_0032 0.49 +0.9 32,195 - Psest_0032 0.49 -1.9 32,195 - Psest_0032 0.49 +0.3 32,195 - Psest_0032 0.49 +1.6 32,197 + Psest_0032 0.50 +0.2 32,197 + Psest_0032 0.50 -0.3 32,197 + Psest_0032 0.50 +0.5 32,197 + Psest_0032 0.50 -0.5 32,197 + Psest_0032 0.50 +0.7 32,197 + Psest_0032 0.50 +0.0 32,197 + Psest_0032 0.50 -0.7 32,197 + Psest_0032 0.50 -2.2 32,197 + Psest_0032 0.50 +1.8 32,198 - Psest_0032 0.50 -0.8 32,198 - Psest_0032 0.50 +0.1 32,198 - Psest_0032 0.50 -0.6 32,198 - Psest_0032 0.50 +0.2 32,198 - Psest_0032 0.50 -2.2 32,198 - Psest_0032 0.50 -0.2 32,198 - Psest_0032 0.50 +1.1 32,279 - Psest_0032 0.58 -1.8 32,301 + Psest_0032 0.61 +1.2 32,341 + Psest_0032 0.65 +1.2 32,341 + Psest_0032 0.65 -0.8 32,341 + Psest_0032 0.65 -1.3 32,341 + Psest_0032 0.65 +2.9 32,341 + Psest_0032 0.65 +2.1 32,341 + Psest_0032 0.65 +0.6 32,342 - Psest_0032 0.65 +0.4 32,342 - Psest_0032 0.65 -0.1 32,342 - Psest_0032 0.65 -1.5 32,342 - Psest_0032 0.65 -1.4 32,342 - Psest_0032 0.65 -0.3 32,342 - Psest_0032 0.65 -2.3 32,342 - Psest_0032 0.65 +0.6 32,342 - Psest_0032 0.65 -0.3 32,342 - +1.2 32,342 - Psest_0032 0.65 +0.4 32,342 - Psest_0032 0.65 -0.4 32,454 + Psest_0032 0.77 -0.3 32,454 + Psest_0032 0.77 -0.1 32,455 - Psest_0032 0.77 +0.5 32,455 - Psest_0032 0.77 -0.2
Or see this region's nucleotide sequence