Experiment: Nalidixic 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2627 and Psest_2628 are separated by 12 nucleotides Psest_2628 and Psest_2629 overlap by 1 nucleotides Psest_2629 and Psest_2630 are separated by 184 nucleotides Psest_2630 and Psest_2631 are separated by 2 nucleotides
Psest_2627: Psest_2627 - hypothetical protein, at 2,809,257 to 2,810,576
_2627
Psest_2628: Psest_2628 - Flp pilus assembly protein, protease CpaA, at 2,810,589 to 2,811,095
_2628
Psest_2629: Psest_2629 - Flp pilus assembly protein, pilin Flp, at 2,811,095 to 2,811,295
_2629
Psest_2630: Psest_2630 - hypothetical protein, at 2,811,480 to 2,811,641
_2630
Psest_2631: Psest_2631 - AraC-type DNA-binding domain-containing proteins, at 2,811,644 to 2,812,516
_2631
Position (kb)
2811
2812 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.568 kb on + strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.569 kb on - strand at 2810.695 kb on + strand, within Psest_2628 at 2810.723 kb on - strand, within Psest_2628 at 2810.844 kb on + strand, within Psest_2628 at 2810.845 kb on - strand, within Psest_2628 at 2811.069 kb on + strand at 2811.069 kb on + strand at 2811.070 kb on - strand at 2811.070 kb on - strand at 2811.070 kb on - strand at 2811.072 kb on + strand at 2811.073 kb on - strand at 2811.073 kb on - strand at 2811.073 kb on - strand at 2811.073 kb on - strand at 2811.073 kb on - strand at 2811.079 kb on - strand at 2811.096 kb on + strand at 2811.156 kb on - strand, within Psest_2629 at 2811.156 kb on - strand, within Psest_2629 at 2811.167 kb on + strand, within Psest_2629 at 2811.188 kb on + strand, within Psest_2629 at 2811.188 kb on + strand, within Psest_2629 at 2811.188 kb on + strand, within Psest_2629 at 2811.189 kb on - strand, within Psest_2629 at 2811.222 kb on + strand, within Psest_2629 at 2811.337 kb on + strand at 2811.384 kb on + strand at 2811.384 kb on + strand at 2811.385 kb on - strand at 2811.385 kb on - strand at 2811.385 kb on - strand at 2811.451 kb on + strand at 2811.451 kb on + strand at 2811.451 kb on + strand at 2811.535 kb on + strand, within Psest_2630 at 2811.535 kb on + strand, within Psest_2630 at 2811.535 kb on + strand, within Psest_2630 at 2811.536 kb on - strand, within Psest_2630 at 2811.541 kb on - strand, within Psest_2630 at 2811.566 kb on - strand, within Psest_2630 at 2811.582 kb on - strand, within Psest_2630 at 2811.639 kb on + strand at 2811.639 kb on + strand at 2811.639 kb on + strand at 2811.639 kb on + strand at 2811.640 kb on - strand at 2811.642 kb on + strand at 2811.642 kb on + strand at 2811.643 kb on - strand at 2811.652 kb on + strand at 2811.653 kb on - strand at 2811.706 kb on + strand at 2811.790 kb on - strand, within Psest_2631 at 2811.790 kb on - strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.962 kb on + strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2811.963 kb on - strand, within Psest_2631 at 2812.254 kb on - strand, within Psest_2631 at 2812.265 kb on + strand, within Psest_2631 at 2812.266 kb on - strand, within Psest_2631 at 2812.349 kb on + strand, within Psest_2631 at 2812.376 kb on + strand, within Psest_2631 at 2812.376 kb on + strand, within Psest_2631 at 2812.376 kb on + strand, within Psest_2631 at 2812.377 kb on - strand, within Psest_2631 at 2812.500 kb on - strand at 2812.563 kb on + strand at 2812.614 kb on + strand at 2812.615 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.00390625 mg/ml remove 2,810,568 + -0.4 2,810,568 + +1.1 2,810,568 + -0.9 2,810,568 + -1.2 2,810,568 + -0.7 2,810,568 + -1.2 2,810,569 - +0.1 2,810,569 - -0.8 2,810,569 - -1.7 2,810,569 - +0.4 2,810,569 - -0.3 2,810,569 - +1.0 2,810,569 - +1.0 2,810,569 - +0.4 2,810,695 + Psest_2628 0.21 -0.1 2,810,723 - Psest_2628 0.26 +0.5 2,810,844 + Psest_2628 0.50 -0.9 2,810,845 - Psest_2628 0.50 +0.1 2,811,069 + +0.3 2,811,069 + +0.6 2,811,070 - -0.3 2,811,070 - -0.0 2,811,070 - -0.3 2,811,072 + +1.9 2,811,073 - +2.3 2,811,073 - +0.8 2,811,073 - -0.1 2,811,073 - +1.4 2,811,073 - -1.1 2,811,079 - +1.2 2,811,096 + +0.4 2,811,156 - Psest_2629 0.30 +2.3 2,811,156 - Psest_2629 0.30 +1.4 2,811,167 + Psest_2629 0.36 +0.1 2,811,188 + Psest_2629 0.46 +0.3 2,811,188 + Psest_2629 0.46 -1.0 2,811,188 + Psest_2629 0.46 +0.1 2,811,189 - Psest_2629 0.47 -0.7 2,811,222 + Psest_2629 0.63 +0.4 2,811,337 + +0.5 2,811,384 + -0.8 2,811,384 + -0.7 2,811,385 - +0.0 2,811,385 - +1.9 2,811,385 - +3.0 2,811,451 + +1.1 2,811,451 + -0.3 2,811,451 + -1.1 2,811,535 + Psest_2630 0.34 +0.7 2,811,535 + Psest_2630 0.34 +2.0 2,811,535 + Psest_2630 0.34 +1.1 2,811,536 - Psest_2630 0.35 -1.4 2,811,541 - Psest_2630 0.38 +0.7 2,811,566 - Psest_2630 0.53 -1.0 2,811,582 - Psest_2630 0.63 +1.0 2,811,639 + -0.7 2,811,639 + +0.9 2,811,639 + +0.4 2,811,639 + -0.0 2,811,640 - -0.7 2,811,642 + +2.9 2,811,642 + -0.9 2,811,643 - -1.7 2,811,652 + -1.0 2,811,653 - +0.1 2,811,706 + +0.5 2,811,790 - Psest_2631 0.17 +0.6 2,811,790 - Psest_2631 0.17 -0.1 2,811,962 + Psest_2631 0.36 +0.9 2,811,962 + Psest_2631 0.36 +0.9 2,811,962 + Psest_2631 0.36 -0.5 2,811,962 + Psest_2631 0.36 +1.3 2,811,962 + Psest_2631 0.36 +1.5 2,811,962 + Psest_2631 0.36 -2.0 2,811,963 - Psest_2631 0.37 -0.2 2,811,963 - Psest_2631 0.37 -0.8 2,811,963 - Psest_2631 0.37 -0.7 2,811,963 - Psest_2631 0.37 +0.5 2,811,963 - Psest_2631 0.37 -2.3 2,812,254 - Psest_2631 0.70 -0.6 2,812,265 + Psest_2631 0.71 +2.2 2,812,266 - Psest_2631 0.71 -0.8 2,812,349 + Psest_2631 0.81 -0.5 2,812,376 + Psest_2631 0.84 -0.3 2,812,376 + Psest_2631 0.84 +0.7 2,812,376 + Psest_2631 0.84 -1.8 2,812,377 - Psest_2631 0.84 -0.2 2,812,500 - +0.3 2,812,563 + -1.8 2,812,614 + +3.1 2,812,615 - +0.8
Or see this region's nucleotide sequence