Experiment: Nalidixic 0.00390625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0150 and Psest_0151 are separated by 357 nucleotides Psest_0151 and Psest_0152 are separated by 129 nucleotides Psest_0152 and Psest_0153 overlap by 1 nucleotides
Psest_0150: Psest_0150 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 148,970 to 150,301
_0150
Psest_0151: Psest_0151 - Response regulator with putative antiterminator output domain, at 150,659 to 151,969
_0151
Psest_0152: Psest_0152 - Imidazoleglycerol-phosphate dehydratase, at 152,099 to 152,692
_0152
Psest_0153: Psest_0153 - imidazole glycerol phosphate synthase, glutamine amidotransferase subunit, at 152,692 to 153,330
_0153
Position (kb) 150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 149.829 kb on + strand, within Psest_0150 at 149.935 kb on - strand, within Psest_0150 at 150.032 kb on + strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.033 kb on - strand, within Psest_0150 at 150.054 kb on + strand, within Psest_0150 at 150.054 kb on + strand, within Psest_0150 at 150.055 kb on - strand, within Psest_0150 at 150.055 kb on - strand, within Psest_0150 at 150.725 kb on + strand at 150.725 kb on + strand at 150.726 kb on - strand at 150.726 kb on - strand at 150.789 kb on + strand at 150.790 kb on - strand, within Psest_0151 at 150.790 kb on - strand, within Psest_0151 at 150.790 kb on - strand, within Psest_0151 at 150.912 kb on - strand, within Psest_0151 at 150.912 kb on - strand, within Psest_0151 at 151.299 kb on - strand, within Psest_0151 at 151.654 kb on - strand, within Psest_0151 at 151.654 kb on - strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.809 kb on + strand, within Psest_0151 at 151.810 kb on - strand, within Psest_0151 at 151.862 kb on - strand at 152.058 kb on + strand at 152.058 kb on + strand at 152.059 kb on - strand at 152.059 kb on - strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.060 kb on + strand at 152.061 kb on - strand at 152.061 kb on - strand at 152.111 kb on - strand at 152.111 kb on - strand at 152.111 kb on - strand at 152.117 kb on + strand at 152.117 kb on + strand at 152.118 kb on - strand at 152.118 kb on - strand at 152.118 kb on - strand at 152.121 kb on + strand at 152.122 kb on - strand at 152.122 kb on - strand at 152.122 kb on - strand at 152.339 kb on - strand, within Psest_0152 at 152.339 kb on - strand, within Psest_0152 at 152.387 kb on + strand, within Psest_0152 at 152.387 kb on + strand, within Psest_0152 at 152.388 kb on - strand, within Psest_0152 at 152.388 kb on - strand, within Psest_0152 at 152.465 kb on + strand, within Psest_0152 at 152.466 kb on - strand, within Psest_0152 at 152.466 kb on - strand, within Psest_0152 at 152.555 kb on - strand, within Psest_0152 at 152.690 kb on + strand at 152.691 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nalidixic 0.00390625 mg/ml remove 149,829 + Psest_0150 0.64 -0.5 149,935 - Psest_0150 0.72 -2.6 150,032 + Psest_0150 0.80 +0.5 150,033 - Psest_0150 0.80 -1.0 150,033 - Psest_0150 0.80 -1.8 150,033 - Psest_0150 0.80 -1.9 150,033 - Psest_0150 0.80 -1.2 150,054 + Psest_0150 0.81 -1.6 150,054 + Psest_0150 0.81 +1.9 150,055 - Psest_0150 0.81 -1.5 150,055 - Psest_0150 0.81 -0.6 150,725 + +0.3 150,725 + -0.2 150,726 - -0.6 150,726 - -0.3 150,789 + +0.5 150,790 - Psest_0151 0.10 +1.3 150,790 - Psest_0151 0.10 -1.4 150,790 - Psest_0151 0.10 +3.1 150,912 - Psest_0151 0.19 -0.5 150,912 - Psest_0151 0.19 -1.4 151,299 - Psest_0151 0.49 +1.7 151,654 - Psest_0151 0.76 -0.4 151,654 - Psest_0151 0.76 -1.0 151,809 + Psest_0151 0.88 -0.5 151,809 + Psest_0151 0.88 +0.2 151,809 + Psest_0151 0.88 +2.5 151,809 + Psest_0151 0.88 +0.0 151,809 + Psest_0151 0.88 -2.0 151,809 + Psest_0151 0.88 -0.1 151,810 - Psest_0151 0.88 -1.0 151,862 - -0.6 152,058 + -0.5 152,058 + -1.6 152,059 - +0.0 152,059 - -2.6 152,060 + +2.1 152,060 + -2.5 152,060 + -0.9 152,060 + -1.2 152,060 + +1.7 152,061 - -1.0 152,061 - -1.7 152,111 - -1.8 152,111 - -0.2 152,111 - -1.1 152,117 + +2.5 152,117 + -1.9 152,118 - -3.0 152,118 - +0.2 152,118 - -2.2 152,121 + +0.9 152,122 - -0.0 152,122 - +0.0 152,122 - +1.8 152,339 - Psest_0152 0.40 -2.2 152,339 - Psest_0152 0.40 -2.0 152,387 + Psest_0152 0.48 -2.0 152,387 + Psest_0152 0.48 +0.4 152,388 - Psest_0152 0.49 -0.1 152,388 - Psest_0152 0.49 -2.0 152,465 + Psest_0152 0.62 +0.5 152,466 - Psest_0152 0.62 +0.0 152,466 - Psest_0152 0.62 +1.8 152,555 - Psest_0152 0.77 +1.5 152,690 + -0.0 152,691 - -0.1
Or see this region's nucleotide sequence