Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4350 and Psest_4352 are separated by 293 nucleotides Psest_4352 and Psest_4353 are separated by 147 nucleotides Psest_4353 and Psest_4354 are separated by 74 nucleotides
Psest_4350: Psest_4350 - Transcriptional regulators of sugar metabolism, at 4,551,006 to 4,551,788
_4350
Psest_4352: Psest_4352 - UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase, at 4,552,082 to 4,553,440
_4352
Psest_4353: Psest_4353 - ATP synthase, F1 epsilon subunit (delta in mitochondria), at 4,553,588 to 4,554,016
_4353
Psest_4354: Psest_4354 - ATP synthase, F1 beta subunit, at 4,554,091 to 4,555,467
_4354
Position (kb)
4552
4553
4554 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4551.226 kb on - strand, within Psest_4350 at 4551.226 kb on - strand, within Psest_4350 at 4551.226 kb on - strand, within Psest_4350 at 4551.243 kb on - strand, within Psest_4350 at 4551.560 kb on - strand, within Psest_4350 at 4551.560 kb on - strand, within Psest_4350 at 4551.560 kb on - strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.576 kb on + strand, within Psest_4350 at 4551.577 kb on - strand, within Psest_4350 at 4551.577 kb on - strand, within Psest_4350 at 4551.577 kb on - strand, within Psest_4350 at 4551.793 kb on - strand at 4553.560 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 4,551,226 - Psest_4350 0.28 -1.5 4,551,226 - Psest_4350 0.28 -1.4 4,551,226 - Psest_4350 0.28 -0.8 4,551,243 - Psest_4350 0.30 -0.9 4,551,560 - Psest_4350 0.71 -0.0 4,551,560 - Psest_4350 0.71 +0.1 4,551,560 - Psest_4350 0.71 -1.3 4,551,576 + Psest_4350 0.73 -0.5 4,551,576 + Psest_4350 0.73 -3.7 4,551,576 + Psest_4350 0.73 -1.7 4,551,576 + Psest_4350 0.73 -3.0 4,551,576 + Psest_4350 0.73 -2.9 4,551,577 - Psest_4350 0.73 +0.6 4,551,577 - Psest_4350 0.73 -1.2 4,551,577 - Psest_4350 0.73 +0.6 4,551,793 - -1.8 4,553,560 - -0.6
Or see this region's nucleotide sequence