Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0336 and Psest_0337 are separated by 92 nucleotides Psest_0337 and Psest_0338 are separated by 219 nucleotides Psest_0338 and Psest_0339 are separated by 163 nucleotides Psest_0339 and Psest_0340 are separated by 63 nucleotides Psest_0340 and Psest_0341 are separated by 20 nucleotides
Psest_0336: Psest_0336 - Protein of unknown function (DUF3509)., at 358,734 to 359,012
_0336
Psest_0337: Psest_0337 - Regulator of cell morphogenesis and NO signaling, at 359,105 to 359,578
_0337
Psest_0338: Psest_0338 - PAS domain S-box/diguanylate cyclase (GGDEF) domain, at 359,798 to 362,008
_0338
Psest_0339: Psest_0339 - hypothetical protein, at 362,172 to 362,501
_0339
Psest_0340: Psest_0340 - hypothetical protein, at 362,565 to 362,747
_0340
Psest_0341: Psest_0341 - flavoprotein, HI0933 family, at 362,768 to 363,952
_0341
Position (kb) 359
360
361
362
363 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 358.994 kb on + strand at 358.995 kb on - strand at 358.995 kb on - strand at 358.998 kb on + strand at 358.998 kb on + strand at 358.998 kb on + strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 358.999 kb on - strand at 359.005 kb on - strand at 359.201 kb on - strand, within Psest_0337 at 359.237 kb on - strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.276 kb on + strand, within Psest_0337 at 359.277 kb on - strand, within Psest_0337 at 359.277 kb on - strand, within Psest_0337 at 359.312 kb on + strand, within Psest_0337 at 359.312 kb on + strand, within Psest_0337 at 359.313 kb on - strand, within Psest_0337 at 359.313 kb on - strand, within Psest_0337 at 359.313 kb on - strand, within Psest_0337 at 359.313 kb on - strand, within Psest_0337 at 359.602 kb on - strand at 359.602 kb on - strand at 359.602 kb on - strand at 359.603 kb on + strand at 359.745 kb on + strand at 359.745 kb on + strand at 359.745 kb on + strand at 359.746 kb on - strand at 359.746 kb on - strand at 359.766 kb on + strand at 359.766 kb on + strand at 359.767 kb on - strand at 359.871 kb on - strand at 359.961 kb on - strand at 360.047 kb on + strand, within Psest_0338 at 360.047 kb on + strand, within Psest_0338 at 360.047 kb on + strand, within Psest_0338 at 360.048 kb on - strand, within Psest_0338 at 360.048 kb on - strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.086 kb on + strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.087 kb on - strand, within Psest_0338 at 360.149 kb on + strand, within Psest_0338 at 360.149 kb on + strand, within Psest_0338 at 360.170 kb on + strand, within Psest_0338 at 360.170 kb on + strand, within Psest_0338 at 360.224 kb on + strand, within Psest_0338 at 360.278 kb on + strand, within Psest_0338 at 360.278 kb on + strand, within Psest_0338 at 360.278 kb on + strand, within Psest_0338 at 360.278 kb on + strand, within Psest_0338 at 360.279 kb on - strand, within Psest_0338 at 360.279 kb on - strand, within Psest_0338 at 360.355 kb on + strand, within Psest_0338 at 360.548 kb on + strand, within Psest_0338 at 360.549 kb on - strand, within Psest_0338 at 360.575 kb on + strand, within Psest_0338 at 360.575 kb on + strand, within Psest_0338 at 360.575 kb on + strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.576 kb on - strand, within Psest_0338 at 360.774 kb on - strand, within Psest_0338 at 361.262 kb on + strand, within Psest_0338 at 361.299 kb on - strand, within Psest_0338 at 361.475 kb on + strand, within Psest_0338 at 361.475 kb on + strand, within Psest_0338 at 361.476 kb on - strand, within Psest_0338 at 361.476 kb on - strand, within Psest_0338 at 361.706 kb on + strand, within Psest_0338 at 361.984 kb on - strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.180 kb on + strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.181 kb on - strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.184 kb on + strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.185 kb on - strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.577 kb on + strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.578 kb on - strand at 362.581 kb on + strand at 362.581 kb on + strand at 362.582 kb on - strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.775 kb on + strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.776 kb on - strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.865 kb on + strand at 362.866 kb on - strand at 362.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 358,994 + -0.7 358,995 - -0.6 358,995 - -0.6 358,998 + +2.0 358,998 + -0.7 358,998 + +0.1 358,999 - -1.0 358,999 - +2.3 358,999 - -1.1 358,999 - +0.4 358,999 - +0.5 358,999 - -2.0 358,999 - -1.8 359,005 - -1.1 359,201 - Psest_0337 0.20 +0.0 359,237 - Psest_0337 0.28 -1.0 359,276 + Psest_0337 0.36 -0.2 359,276 + Psest_0337 0.36 +0.2 359,276 + Psest_0337 0.36 -0.3 359,276 + Psest_0337 0.36 -0.5 359,276 + Psest_0337 0.36 +0.2 359,276 + Psest_0337 0.36 -0.6 359,277 - Psest_0337 0.36 -0.1 359,277 - Psest_0337 0.36 +0.9 359,312 + Psest_0337 0.44 -0.5 359,312 + Psest_0337 0.44 +0.3 359,313 - Psest_0337 0.44 -1.5 359,313 - Psest_0337 0.44 +0.5 359,313 - Psest_0337 0.44 +0.5 359,313 - Psest_0337 0.44 +0.1 359,602 - -0.5 359,602 - -0.2 359,602 - -0.7 359,603 + -2.3 359,745 + +1.0 359,745 + -0.8 359,745 + -0.4 359,746 - +1.8 359,746 - -1.5 359,766 + +2.4 359,766 + -1.9 359,767 - -1.3 359,871 - +0.0 359,961 - -0.5 360,047 + Psest_0338 0.11 +1.7 360,047 + Psest_0338 0.11 +2.2 360,047 + Psest_0338 0.11 +1.3 360,048 - Psest_0338 0.11 +0.0 360,048 - Psest_0338 0.11 -1.7 360,086 + Psest_0338 0.13 +2.0 360,086 + Psest_0338 0.13 -1.1 360,086 + Psest_0338 0.13 -0.3 360,086 + Psest_0338 0.13 -0.1 360,086 + Psest_0338 0.13 -1.7 360,086 + Psest_0338 0.13 -2.1 360,086 + Psest_0338 0.13 +2.3 360,086 + Psest_0338 0.13 +0.3 360,086 + Psest_0338 0.13 -0.4 360,086 + Psest_0338 0.13 -1.3 360,087 - Psest_0338 0.13 -0.2 360,087 - Psest_0338 0.13 +1.4 360,087 - Psest_0338 0.13 +2.2 360,087 - Psest_0338 0.13 +0.4 360,087 - Psest_0338 0.13 -0.3 360,087 - Psest_0338 0.13 +1.2 360,087 - Psest_0338 0.13 -0.3 360,087 - Psest_0338 0.13 -2.1 360,149 + Psest_0338 0.16 +1.4 360,149 + Psest_0338 0.16 +0.4 360,170 + Psest_0338 0.17 -0.4 360,170 + Psest_0338 0.17 +1.7 360,224 + Psest_0338 0.19 -0.3 360,278 + Psest_0338 0.22 +0.4 360,278 + Psest_0338 0.22 -1.0 360,278 + Psest_0338 0.22 +0.9 360,278 + Psest_0338 0.22 +2.1 360,279 - Psest_0338 0.22 +0.8 360,279 - Psest_0338 0.22 +0.2 360,355 + Psest_0338 0.25 +2.1 360,548 + Psest_0338 0.34 +0.1 360,549 - Psest_0338 0.34 +0.1 360,575 + Psest_0338 0.35 -1.6 360,575 + Psest_0338 0.35 -1.1 360,575 + Psest_0338 0.35 +0.6 360,576 - Psest_0338 0.35 +2.6 360,576 - Psest_0338 0.35 -0.5 360,576 - Psest_0338 0.35 -0.2 360,576 - Psest_0338 0.35 -0.5 360,576 - Psest_0338 0.35 +1.4 360,576 - Psest_0338 0.35 +0.7 360,774 - Psest_0338 0.44 -0.3 361,262 + Psest_0338 0.66 +0.2 361,299 - Psest_0338 0.68 +1.5 361,475 + Psest_0338 0.76 -0.7 361,475 + Psest_0338 0.76 -2.5 361,476 - Psest_0338 0.76 -0.0 361,476 - Psest_0338 0.76 +0.6 361,706 + Psest_0338 0.86 +2.7 361,984 - -1.0 362,180 + +0.2 362,180 + -0.2 362,180 + -2.0 362,180 + +1.9 362,180 + +0.6 362,180 + -0.5 362,180 + +1.8 362,180 + +3.2 362,180 + -0.8 362,181 - +2.1 362,181 - -0.2 362,181 - -1.0 362,181 - +1.4 362,181 - -0.2 362,181 - -0.5 362,184 + +1.2 362,184 + +0.2 362,184 + +1.6 362,184 + -1.9 362,184 + -0.8 362,185 - -0.2 362,185 - -1.1 362,185 - +1.7 362,185 - +0.8 362,577 + -0.9 362,577 + -1.2 362,577 + +0.9 362,577 + -1.5 362,578 - +0.2 362,578 - +1.6 362,578 - +0.4 362,578 - +0.5 362,578 - -0.5 362,578 - -2.1 362,578 - +1.0 362,578 - +0.3 362,578 - -1.5 362,581 + -1.8 362,581 + +1.0 362,582 - +3.8 362,775 + +0.9 362,775 + +0.2 362,775 + +0.6 362,775 + -0.4 362,775 + +0.2 362,775 + -0.5 362,776 - +1.1 362,776 - -0.7 362,776 - -0.8 362,776 - +1.0 362,776 - -0.6 362,776 - +1.3 362,865 + -2.5 362,865 + -0.3 362,865 + +0.6 362,865 + +0.2 362,865 + +0.4 362,865 + +0.7 362,866 - +1.6 362,866 - +2.5
Or see this region's nucleotide sequence