Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2297 and Psest_2298 are separated by 150 nucleotides Psest_2298 and Psest_2299 are separated by 51 nucleotides Psest_2299 and Psest_2300 are separated by 32 nucleotides Psest_2300 and Psest_2301 are separated by 273 nucleotides
Psest_2297: Psest_2297 - hypothetical protein, at 2,448,974 to 2,450,401
_2297
Psest_2298: Psest_2298 - tRNA_Leu_TAG, at 2,450,552 to 2,450,636
_2298
Psest_2299: Psest_2299 - tRNA_His_GTG, at 2,450,688 to 2,450,763
_2299
Psest_2300: Psest_2300 - tRNA_Arg_TCT, at 2,450,796 to 2,450,872
_2300
Psest_2301: Psest_2301 - 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase, at 2,451,146 to 2,452,000
_2301
Position (kb)
2450
2451 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2450.018 kb on + strand, within Psest_2297 at 2450.018 kb on + strand, within Psest_2297 at 2450.184 kb on + strand, within Psest_2297 at 2450.185 kb on - strand, within Psest_2297 at 2450.452 kb on + strand at 2450.453 kb on - strand at 2450.456 kb on + strand at 2450.456 kb on + strand at 2450.457 kb on - strand at 2450.457 kb on - strand at 2450.529 kb on + strand at 2450.530 kb on - strand at 2450.530 kb on - strand at 2450.876 kb on - strand at 2451.037 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 2,450,018 + Psest_2297 0.73 +0.3 2,450,018 + Psest_2297 0.73 -0.3 2,450,184 + Psest_2297 0.85 +0.0 2,450,185 - Psest_2297 0.85 +1.6 2,450,452 + -0.4 2,450,453 - -1.1 2,450,456 + +1.6 2,450,456 + -2.6 2,450,457 - -0.7 2,450,457 - +2.0 2,450,529 + +3.2 2,450,530 - +2.2 2,450,530 - -1.9 2,450,876 - -1.0 2,451,037 + +2.3
Or see this region's nucleotide sequence