Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2273 and Psest_2274 are separated by 46 nucleotides Psest_2274 and Psest_2275 are separated by 178 nucleotides
Psest_2273: Psest_2273 - coenzyme PQQ biosynthesis protein B, at 2,422,646 to 2,423,557
_2273
Psest_2274: Psest_2274 - coenzyme PQQ biosynthesis protein A, at 2,423,604 to 2,423,675
_2274
Psest_2275: Psest_2275 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 2,423,854 to 2,424,972
_2275
Position (kb)
2423
2424 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2422.908 kb on - strand, within Psest_2273 at 2422.971 kb on + strand, within Psest_2273 at 2422.971 kb on + strand, within Psest_2273 at 2422.972 kb on - strand, within Psest_2273 at 2422.993 kb on + strand, within Psest_2273 at 2422.993 kb on + strand, within Psest_2273 at 2422.993 kb on + strand, within Psest_2273 at 2422.994 kb on - strand, within Psest_2273 at 2423.072 kb on - strand, within Psest_2273 at 2423.072 kb on - strand, within Psest_2273 at 2423.092 kb on + strand, within Psest_2273 at 2423.285 kb on + strand, within Psest_2273 at 2423.285 kb on + strand, within Psest_2273 at 2423.285 kb on + strand, within Psest_2273 at 2423.286 kb on - strand, within Psest_2273 at 2423.286 kb on - strand, within Psest_2273 at 2423.540 kb on + strand at 2423.540 kb on + strand at 2423.540 kb on + strand at 2423.620 kb on + strand, within Psest_2274 at 2423.620 kb on + strand, within Psest_2274 at 2423.620 kb on + strand, within Psest_2274 at 2423.621 kb on - strand, within Psest_2274 at 2423.621 kb on - strand, within Psest_2274 at 2423.621 kb on - strand, within Psest_2274 at 2423.621 kb on - strand, within Psest_2274 at 2423.666 kb on + strand, within Psest_2274 at 2423.667 kb on - strand, within Psest_2274 at 2423.667 kb on - strand, within Psest_2274 at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.676 kb on - strand at 2423.693 kb on - strand at 2423.693 kb on - strand at 2423.722 kb on + strand at 2423.723 kb on - strand at 2423.723 kb on - strand at 2423.723 kb on - strand at 2423.723 kb on - strand at 2423.740 kb on + strand at 2423.753 kb on + strand at 2423.785 kb on - strand at 2423.812 kb on + strand at 2423.812 kb on + strand at 2423.813 kb on - strand at 2423.813 kb on - strand at 2423.974 kb on - strand, within Psest_2275 at 2423.974 kb on - strand, within Psest_2275 at 2424.014 kb on - strand, within Psest_2275 at 2424.046 kb on + strand, within Psest_2275 at 2424.292 kb on - strand, within Psest_2275
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 2,422,908 - Psest_2273 0.29 +0.5 2,422,971 + Psest_2273 0.36 +1.5 2,422,971 + Psest_2273 0.36 -0.7 2,422,972 - Psest_2273 0.36 +1.3 2,422,993 + Psest_2273 0.38 -0.9 2,422,993 + Psest_2273 0.38 -1.0 2,422,993 + Psest_2273 0.38 -1.2 2,422,994 - Psest_2273 0.38 -0.0 2,423,072 - Psest_2273 0.47 -2.7 2,423,072 - Psest_2273 0.47 +3.1 2,423,092 + Psest_2273 0.49 +1.0 2,423,285 + Psest_2273 0.70 +1.1 2,423,285 + Psest_2273 0.70 -0.2 2,423,285 + Psest_2273 0.70 -0.7 2,423,286 - Psest_2273 0.70 +2.4 2,423,286 - Psest_2273 0.70 -1.8 2,423,540 + +1.4 2,423,540 + -2.1 2,423,540 + +1.2 2,423,620 + Psest_2274 0.22 +0.0 2,423,620 + Psest_2274 0.22 +0.7 2,423,620 + Psest_2274 0.22 -1.3 2,423,621 - Psest_2274 0.24 -1.3 2,423,621 - Psest_2274 0.24 +0.1 2,423,621 - Psest_2274 0.24 -0.4 2,423,621 - Psest_2274 0.24 -1.2 2,423,666 + Psest_2274 0.86 +0.6 2,423,667 - Psest_2274 0.88 -2.4 2,423,667 - Psest_2274 0.88 -0.3 2,423,676 - +0.7 2,423,676 - -0.3 2,423,676 - +1.7 2,423,676 - +3.4 2,423,676 - +0.3 2,423,676 - -2.4 2,423,676 - -0.6 2,423,676 - -0.6 2,423,676 - -1.8 2,423,676 - -0.4 2,423,676 - +0.7 2,423,676 - -0.5 2,423,676 - +0.0 2,423,693 - -0.7 2,423,693 - -0.4 2,423,722 + -0.4 2,423,723 - +0.5 2,423,723 - +1.0 2,423,723 - -2.8 2,423,723 - +0.6 2,423,740 + +1.4 2,423,753 + +0.4 2,423,785 - +0.5 2,423,812 + +0.3 2,423,812 + +0.4 2,423,813 - -1.2 2,423,813 - -0.3 2,423,974 - Psest_2275 0.11 +1.7 2,423,974 - Psest_2275 0.11 +0.6 2,424,014 - Psest_2275 0.14 -0.8 2,424,046 + Psest_2275 0.17 +0.1 2,424,292 - Psest_2275 0.39 +0.8
Or see this region's nucleotide sequence