Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2083 and Psest_2084 are separated by 58 nucleotides Psest_2084 and Psest_2085 are separated by 181 nucleotides Psest_2085 and Psest_2086 are separated by 13 nucleotides
Psest_2083: Psest_2083 - Putative sterol carrier protein, at 2,212,845 to 2,213,159
_2083
Psest_2084: Psest_2084 - Fructose-2,6-bisphosphatase, at 2,213,218 to 2,213,916
_2084
Psest_2085: Psest_2085 - Periplasmic serine proteases (ClpP class), at 2,214,098 to 2,215,120
_2085
Psest_2086: Psest_2086 - putative quinone oxidoreductase, YhdH/YhfP family, at 2,215,134 to 2,216,126
_2086
Position (kb)
2214
2215
2216 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2213.223 kb on + strand at 2213.223 kb on + strand at 2213.223 kb on + strand at 2213.223 kb on + strand at 2213.223 kb on + strand at 2213.224 kb on - strand at 2213.224 kb on - strand at 2213.272 kb on - strand at 2213.272 kb on - strand at 2213.272 kb on - strand at 2213.390 kb on - strand, within Psest_2084 at 2213.555 kb on + strand, within Psest_2084 at 2213.555 kb on + strand, within Psest_2084 at 2213.555 kb on + strand, within Psest_2084 at 2213.555 kb on + strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.556 kb on - strand, within Psest_2084 at 2213.607 kb on + strand, within Psest_2084 at 2213.811 kb on - strand, within Psest_2084 at 2213.861 kb on + strand at 2213.903 kb on + strand at 2213.903 kb on + strand at 2213.903 kb on + strand at 2213.903 kb on + strand at 2213.904 kb on - strand at 2213.953 kb on + strand at 2214.092 kb on + strand at 2214.092 kb on + strand at 2214.092 kb on + strand at 2214.093 kb on - strand at 2214.095 kb on + strand at 2214.095 kb on + strand at 2214.095 kb on + strand at 2214.096 kb on - strand at 2214.096 kb on - strand at 2214.096 kb on - strand at 2214.251 kb on + strand, within Psest_2085 at 2214.516 kb on - strand, within Psest_2085 at 2214.605 kb on + strand, within Psest_2085 at 2214.749 kb on + strand, within Psest_2085 at 2214.824 kb on - strand, within Psest_2085 at 2214.962 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.007 kb on + strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.008 kb on - strand, within Psest_2085 at 2215.011 kb on + strand, within Psest_2085 at 2215.011 kb on + strand, within Psest_2085 at 2215.012 kb on - strand, within Psest_2085 at 2215.012 kb on - strand, within Psest_2085 at 2215.012 kb on - strand, within Psest_2085 at 2215.012 kb on - strand, within Psest_2085 at 2215.060 kb on + strand at 2215.153 kb on + strand at 2215.251 kb on + strand, within Psest_2086 at 2215.280 kb on - strand, within Psest_2086 at 2215.314 kb on + strand, within Psest_2086 at 2215.446 kb on + strand, within Psest_2086 at 2215.516 kb on + strand, within Psest_2086 at 2215.517 kb on - strand, within Psest_2086 at 2215.628 kb on + strand, within Psest_2086 at 2215.659 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.661 kb on + strand, within Psest_2086 at 2215.662 kb on - strand, within Psest_2086 at 2215.662 kb on - strand, within Psest_2086 at 2215.662 kb on - strand, within Psest_2086 at 2215.675 kb on + strand, within Psest_2086 at 2215.916 kb on + strand, within Psest_2086 at 2215.916 kb on + strand, within Psest_2086 at 2216.113 kb on + strand at 2216.113 kb on + strand at 2216.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 2,213,223 + -0.4 2,213,223 + -1.4 2,213,223 + +0.9 2,213,223 + -0.6 2,213,223 + +0.9 2,213,224 - -0.3 2,213,224 - -3.0 2,213,272 - -0.4 2,213,272 - -1.8 2,213,272 - +0.8 2,213,390 - Psest_2084 0.25 -0.3 2,213,555 + Psest_2084 0.48 +0.2 2,213,555 + Psest_2084 0.48 -0.5 2,213,555 + Psest_2084 0.48 +0.7 2,213,555 + Psest_2084 0.48 +0.0 2,213,556 - Psest_2084 0.48 -0.3 2,213,556 - Psest_2084 0.48 +2.5 2,213,556 - Psest_2084 0.48 -1.9 2,213,556 - Psest_2084 0.48 -0.9 2,213,556 - Psest_2084 0.48 +1.1 2,213,556 - Psest_2084 0.48 -0.9 2,213,607 + Psest_2084 0.56 +2.9 2,213,811 - Psest_2084 0.85 +0.8 2,213,861 + -0.8 2,213,903 + -0.3 2,213,903 + +0.4 2,213,903 + +1.1 2,213,903 + -0.0 2,213,904 - -1.2 2,213,953 + +2.1 2,214,092 + -1.3 2,214,092 + -0.4 2,214,092 + -2.5 2,214,093 - +3.1 2,214,095 + +1.2 2,214,095 + -0.1 2,214,095 + -2.0 2,214,096 - -0.8 2,214,096 - -2.4 2,214,096 - -0.4 2,214,251 + Psest_2085 0.15 -0.7 2,214,516 - Psest_2085 0.41 -0.5 2,214,605 + Psest_2085 0.50 +1.4 2,214,749 + Psest_2085 0.64 +1.1 2,214,824 - Psest_2085 0.71 +0.3 2,214,962 + Psest_2085 0.84 +0.1 2,215,007 + Psest_2085 0.89 +0.8 2,215,007 + Psest_2085 0.89 -0.5 2,215,007 + Psest_2085 0.89 +0.6 2,215,007 + Psest_2085 0.89 +0.4 2,215,007 + Psest_2085 0.89 -1.0 2,215,007 + Psest_2085 0.89 -0.6 2,215,007 + Psest_2085 0.89 -0.8 2,215,008 - Psest_2085 0.89 -1.1 2,215,008 - Psest_2085 0.89 -0.1 2,215,008 - Psest_2085 0.89 -1.2 2,215,008 - Psest_2085 0.89 -0.8 2,215,008 - Psest_2085 0.89 -1.5 2,215,008 - Psest_2085 0.89 -1.0 2,215,011 + Psest_2085 0.89 -1.0 2,215,011 + Psest_2085 0.89 -0.7 2,215,012 - Psest_2085 0.89 -0.4 2,215,012 - Psest_2085 0.89 -1.3 2,215,012 - Psest_2085 0.89 -1.2 2,215,012 - Psest_2085 0.89 +1.8 2,215,060 + +1.3 2,215,153 + -0.3 2,215,251 + Psest_2086 0.12 -0.8 2,215,280 - Psest_2086 0.15 +0.5 2,215,314 + Psest_2086 0.18 -0.5 2,215,446 + Psest_2086 0.31 +0.4 2,215,516 + Psest_2086 0.38 +0.5 2,215,517 - Psest_2086 0.39 +2.2 2,215,628 + Psest_2086 0.50 -0.9 2,215,659 + Psest_2086 0.53 -0.3 2,215,661 + Psest_2086 0.53 -1.2 2,215,661 + Psest_2086 0.53 +1.4 2,215,661 + Psest_2086 0.53 +1.1 2,215,661 + Psest_2086 0.53 +0.5 2,215,661 + Psest_2086 0.53 +0.2 2,215,661 + Psest_2086 0.53 +1.6 2,215,662 - Psest_2086 0.53 +1.0 2,215,662 - Psest_2086 0.53 -1.1 2,215,662 - Psest_2086 0.53 +0.9 2,215,675 + Psest_2086 0.54 -2.1 2,215,916 + Psest_2086 0.79 -1.8 2,215,916 + Psest_2086 0.79 +0.5 2,216,113 + +0.1 2,216,113 + -0.8 2,216,114 - +0.2
Or see this region's nucleotide sequence