Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2062 and Psest_2063 are separated by 63 nucleotides Psest_2063 and Psest_2064 overlap by 20 nucleotides Psest_2064 and Psest_2065 are separated by 1 nucleotides
Psest_2062: Psest_2062 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,187,981 to 2,188,745
_2062
Psest_2063: Psest_2063 - Enoyl-[acyl-carrier-protein] reductase (NADH), at 2,188,809 to 2,189,603
_2063
Psest_2064: Psest_2064 - ABC-type uncharacterized transport system, duplicated ATPase component, at 2,189,584 to 2,191,245
_2064
Psest_2065: Psest_2065 - ABC-type uncharacterized transport system, permease component, at 2,191,247 to 2,192,266
_2065
Position (kb)
2189
2190
2191
2192 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 2188.790 kb on + strand at 2188.791 kb on - strand at 2188.794 kb on + strand at 2189.674 kb on - strand at 2189.674 kb on - strand at 2190.551 kb on - strand, within Psest_2064 at 2191.249 kb on - strand at 2191.249 kb on - strand at 2191.249 kb on - strand at 2191.566 kb on + strand, within Psest_2065 at 2191.695 kb on - strand, within Psest_2065 at 2191.699 kb on - strand, within Psest_2065
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 2,188,790 + -1.1 2,188,791 - -1.0 2,188,794 + -1.1 2,189,674 - +2.0 2,189,674 - +3.9 2,190,551 - Psest_2064 0.58 +6.1 2,191,249 - +2.7 2,191,249 - -0.0 2,191,249 - +5.0 2,191,566 + Psest_2065 0.31 +3.2 2,191,695 - Psest_2065 0.44 +3.7 2,191,699 - Psest_2065 0.44 +4.7
Or see this region's nucleotide sequence