Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2021 and Psest_2022 are separated by 69 nucleotides Psest_2022 and Psest_2023 are separated by 102 nucleotides Psest_2023 and Psest_2024 are separated by 360 nucleotides
Psest_2021: Psest_2021 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, at 2,140,157 to 2,141,284
_2021
Psest_2022: Psest_2022 - ADP-ribose pyrophosphatase, at 2,141,354 to 2,141,794
_2022
Psest_2023: Psest_2023 - isocitrate dehydrogenase, NADP-dependent, monomeric type, at 2,141,897 to 2,144,125
_2023
Psest_2024: Psest_2024 - isocitrate dehydrogenase, NADP-dependent, prokaryotic type, at 2,144,486 to 2,145,742
_2024
Position (kb)
2141
2142
2143
2144
2145 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2141.285 kb on - strand at 2141.553 kb on + strand, within Psest_2022 at 2141.553 kb on + strand, within Psest_2022 at 2141.580 kb on + strand, within Psest_2022 at 2141.581 kb on - strand, within Psest_2022 at 2141.586 kb on + strand, within Psest_2022 at 2141.587 kb on - strand, within Psest_2022 at 2141.587 kb on - strand, within Psest_2022 at 2141.587 kb on - strand, within Psest_2022 at 2141.777 kb on - strand at 2141.794 kb on + strand at 2141.795 kb on - strand at 2141.810 kb on + strand at 2141.810 kb on + strand at 2141.810 kb on + strand at 2141.811 kb on - strand at 2141.811 kb on - strand at 2142.394 kb on - strand, within Psest_2023 at 2142.394 kb on - strand, within Psest_2023 at 2142.481 kb on - strand, within Psest_2023 at 2142.613 kb on - strand, within Psest_2023 at 2142.613 kb on - strand, within Psest_2023 at 2143.159 kb on - strand, within Psest_2023 at 2143.281 kb on + strand, within Psest_2023 at 2143.302 kb on + strand, within Psest_2023 at 2143.599 kb on + strand, within Psest_2023 at 2143.600 kb on - strand, within Psest_2023 at 2143.663 kb on - strand, within Psest_2023 at 2143.746 kb on + strand, within Psest_2023 at 2144.045 kb on + strand at 2144.045 kb on + strand at 2144.100 kb on + strand at 2144.100 kb on + strand at 2144.127 kb on + strand at 2144.246 kb on + strand at 2144.314 kb on - strand at 2144.359 kb on - strand at 2144.361 kb on - strand at 2144.398 kb on + strand at 2144.399 kb on - strand at 2144.415 kb on + strand at 2144.415 kb on + strand at 2144.416 kb on - strand at 2144.416 kb on - strand at 2144.416 kb on - strand at 2144.416 kb on - strand at 2144.416 kb on - strand at 2144.582 kb on + strand at 2144.582 kb on + strand at 2144.582 kb on + strand at 2144.583 kb on - strand at 2144.583 kb on - strand at 2144.583 kb on - strand at 2144.583 kb on - strand at 2144.584 kb on + strand at 2144.584 kb on + strand at 2144.584 kb on + strand at 2144.584 kb on + strand at 2144.584 kb on + strand at 2144.585 kb on - strand at 2144.585 kb on - strand at 2144.585 kb on - strand at 2144.585 kb on - strand at 2144.585 kb on - strand at 2144.585 kb on - strand at 2144.585 kb on - strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.586 kb on + strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.587 kb on - strand at 2144.661 kb on - strand, within Psest_2024 at 2144.774 kb on + strand, within Psest_2024 at 2144.774 kb on + strand, within Psest_2024 at 2144.775 kb on - strand, within Psest_2024 at 2144.775 kb on - strand, within Psest_2024 at 2144.775 kb on - strand, within Psest_2024 at 2144.864 kb on + strand, within Psest_2024 at 2144.864 kb on + strand, within Psest_2024 at 2144.865 kb on - strand, within Psest_2024 at 2144.865 kb on - strand, within Psest_2024 at 2144.865 kb on - strand, within Psest_2024 at 2144.969 kb on + strand, within Psest_2024 at 2144.970 kb on - strand, within Psest_2024 at 2144.970 kb on - strand, within Psest_2024 at 2144.970 kb on - strand, within Psest_2024 at 2144.980 kb on - strand, within Psest_2024
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 2,141,285 - +0.2 2,141,553 + Psest_2022 0.45 -0.6 2,141,553 + Psest_2022 0.45 -0.0 2,141,580 + Psest_2022 0.51 -0.4 2,141,581 - Psest_2022 0.51 +1.0 2,141,586 + Psest_2022 0.53 +0.7 2,141,587 - Psest_2022 0.53 -0.0 2,141,587 - Psest_2022 0.53 +1.9 2,141,587 - Psest_2022 0.53 -0.2 2,141,777 - -3.5 2,141,794 + -1.7 2,141,795 - +1.0 2,141,810 + -0.2 2,141,810 + +1.1 2,141,810 + +0.8 2,141,811 - +0.8 2,141,811 - -2.5 2,142,394 - Psest_2023 0.22 -2.6 2,142,394 - Psest_2023 0.22 -1.5 2,142,481 - Psest_2023 0.26 -1.2 2,142,613 - Psest_2023 0.32 -2.5 2,142,613 - Psest_2023 0.32 -1.6 2,143,159 - Psest_2023 0.57 +1.0 2,143,281 + Psest_2023 0.62 -0.6 2,143,302 + Psest_2023 0.63 -2.5 2,143,599 + Psest_2023 0.76 -1.6 2,143,600 - Psest_2023 0.76 +0.3 2,143,663 - Psest_2023 0.79 -0.6 2,143,746 + Psest_2023 0.83 -1.1 2,144,045 + -1.7 2,144,045 + +0.5 2,144,100 + -0.2 2,144,100 + -2.5 2,144,127 + -1.3 2,144,246 + -1.5 2,144,314 - -0.2 2,144,359 - -0.5 2,144,361 - -1.3 2,144,398 + -1.0 2,144,399 - -1.4 2,144,415 + -1.1 2,144,415 + -3.9 2,144,416 - +0.9 2,144,416 - +1.3 2,144,416 - -1.6 2,144,416 - +0.5 2,144,416 - +0.8 2,144,582 + +1.0 2,144,582 + -1.4 2,144,582 + -0.0 2,144,583 - +0.3 2,144,583 - -0.3 2,144,583 - +1.7 2,144,583 - -0.2 2,144,584 + -1.3 2,144,584 + -1.3 2,144,584 + +0.0 2,144,584 + -0.3 2,144,584 + -1.8 2,144,585 - +0.8 2,144,585 - +0.2 2,144,585 - +1.6 2,144,585 - +1.7 2,144,585 - +0.6 2,144,585 - -1.5 2,144,585 - +3.3 2,144,586 + -2.1 2,144,586 + -0.6 2,144,586 + -1.8 2,144,586 + -1.2 2,144,586 + -1.1 2,144,586 + -1.6 2,144,586 + -2.5 2,144,586 + -1.4 2,144,586 + -1.0 2,144,586 + -0.4 2,144,586 + +0.4 2,144,586 + -2.9 2,144,587 - +1.7 2,144,587 - +2.7 2,144,587 - +0.6 2,144,587 - +3.4 2,144,587 - -0.7 2,144,587 - +1.0 2,144,587 - -1.2 2,144,587 - -0.3 2,144,587 - -0.5 2,144,587 - +1.3 2,144,587 - +0.3 2,144,661 - Psest_2024 0.14 +1.3 2,144,774 + Psest_2024 0.23 +1.3 2,144,774 + Psest_2024 0.23 -1.0 2,144,775 - Psest_2024 0.23 +2.3 2,144,775 - Psest_2024 0.23 -1.3 2,144,775 - Psest_2024 0.23 -0.7 2,144,864 + Psest_2024 0.30 -0.5 2,144,864 + Psest_2024 0.30 -0.3 2,144,865 - Psest_2024 0.30 +0.7 2,144,865 - Psest_2024 0.30 -0.7 2,144,865 - Psest_2024 0.30 +0.1 2,144,969 + Psest_2024 0.38 +0.8 2,144,970 - Psest_2024 0.39 +0.8 2,144,970 - Psest_2024 0.39 -1.2 2,144,970 - Psest_2024 0.39 +2.1 2,144,980 - Psest_2024 0.39 +0.1
Or see this region's nucleotide sequence