Experiment: Spectinomycin 0.00625 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1632 and Psest_1633 are separated by 179 nucleotides Psest_1633 and Psest_1634 are separated by 103 nucleotides Psest_1634 and Psest_1635 are separated by 68 nucleotides
Psest_1632: Psest_1632 - Predicted permeases, at 1,732,605 to 1,733,360
_1632
Psest_1633: Psest_1633 - Protein of unknown function (DUF3365)., at 1,733,540 to 1,734,097
_1633
Psest_1634: Psest_1634 - tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain, at 1,734,201 to 1,736,183
_1634
Psest_1635: Psest_1635 - Signal transduction histidine kinase, at 1,736,252 to 1,739,005
_1635
Position (kb)
1734
1735
1736
1737 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1733.352 kb on + strand at 1733.352 kb on + strand at 1733.352 kb on + strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.353 kb on - strand at 1733.461 kb on + strand at 1733.461 kb on + strand at 1733.461 kb on + strand at 1733.462 kb on - strand at 1733.462 kb on - strand at 1733.462 kb on - strand at 1733.511 kb on + strand at 1733.639 kb on + strand, within Psest_1633 at 1733.639 kb on + strand, within Psest_1633 at 1733.639 kb on + strand, within Psest_1633 at 1733.639 kb on + strand, within Psest_1633 at 1733.640 kb on - strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.642 kb on + strand, within Psest_1633 at 1733.643 kb on - strand, within Psest_1633 at 1733.643 kb on - strand, within Psest_1633 at 1733.643 kb on - strand, within Psest_1633 at 1733.709 kb on + strand, within Psest_1633 at 1733.710 kb on - strand, within Psest_1633 at 1734.056 kb on + strand at 1734.109 kb on + strand at 1734.110 kb on - strand at 1734.206 kb on + strand at 1734.365 kb on - strand at 1734.431 kb on - strand, within Psest_1634 at 1734.431 kb on - strand, within Psest_1634 at 1734.481 kb on + strand, within Psest_1634 at 1734.560 kb on - strand, within Psest_1634 at 1734.691 kb on + strand, within Psest_1634 at 1734.691 kb on + strand, within Psest_1634 at 1735.195 kb on + strand, within Psest_1634 at 1735.255 kb on + strand, within Psest_1634 at 1735.255 kb on + strand, within Psest_1634 at 1735.255 kb on + strand, within Psest_1634 at 1735.256 kb on - strand, within Psest_1634 at 1735.256 kb on - strand, within Psest_1634 at 1735.713 kb on + strand, within Psest_1634 at 1735.713 kb on + strand, within Psest_1634 at 1735.714 kb on - strand, within Psest_1634 at 1735.714 kb on - strand, within Psest_1634 at 1735.714 kb on - strand, within Psest_1634 at 1735.714 kb on - strand, within Psest_1634 at 1735.792 kb on + strand, within Psest_1634 at 1735.792 kb on + strand, within Psest_1634 at 1735.793 kb on - strand, within Psest_1634 at 1735.793 kb on - strand, within Psest_1634 at 1735.793 kb on - strand, within Psest_1634 at 1735.793 kb on - strand, within Psest_1634 at 1735.793 kb on - strand, within Psest_1634 at 1736.089 kb on + strand at 1736.089 kb on + strand at 1736.089 kb on + strand at 1736.090 kb on - strand at 1736.090 kb on - strand at 1736.253 kb on + strand at 1736.253 kb on + strand at 1736.270 kb on + strand at 1736.654 kb on + strand, within Psest_1635 at 1736.654 kb on + strand, within Psest_1635 at 1736.654 kb on + strand, within Psest_1635 at 1736.697 kb on + strand, within Psest_1635 at 1736.697 kb on + strand, within Psest_1635 at 1736.698 kb on - strand, within Psest_1635 at 1736.698 kb on - strand, within Psest_1635 at 1736.698 kb on - strand, within Psest_1635 at 1736.698 kb on - strand, within Psest_1635 at 1736.698 kb on - strand, within Psest_1635 at 1737.040 kb on - strand, within Psest_1635 at 1737.086 kb on - strand, within Psest_1635 at 1737.086 kb on - strand, within Psest_1635 at 1737.086 kb on - strand, within Psest_1635 at 1737.086 kb on - strand, within Psest_1635 at 1737.118 kb on + strand, within Psest_1635 at 1737.118 kb on + strand, within Psest_1635 at 1737.119 kb on - strand, within Psest_1635 at 1737.119 kb on - strand, within Psest_1635 at 1737.119 kb on - strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.120 kb on + strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635 at 1737.121 kb on - strand, within Psest_1635
Per-strain Table
Position Strand Gene LocusTag Fraction Spectinomycin 0.00625 mg/ml remove 1,733,352 + +0.5 1,733,352 + -0.5 1,733,352 + +0.6 1,733,353 - +0.1 1,733,353 - +0.9 1,733,353 - -0.6 1,733,353 - -0.0 1,733,353 - +1.1 1,733,353 - -1.3 1,733,353 - +2.3 1,733,353 - -1.8 1,733,461 + -1.7 1,733,461 + +1.1 1,733,461 + +1.2 1,733,462 - -0.1 1,733,462 - -0.3 1,733,462 - -0.6 1,733,511 + +1.8 1,733,639 + Psest_1633 0.18 -3.6 1,733,639 + Psest_1633 0.18 -0.3 1,733,639 + Psest_1633 0.18 -0.5 1,733,639 + Psest_1633 0.18 -1.5 1,733,640 - Psest_1633 0.18 -0.2 1,733,642 + Psest_1633 0.18 +0.7 1,733,642 + Psest_1633 0.18 -1.6 1,733,642 + Psest_1633 0.18 +1.2 1,733,642 + Psest_1633 0.18 -1.8 1,733,642 + Psest_1633 0.18 -1.3 1,733,642 + Psest_1633 0.18 -1.1 1,733,642 + Psest_1633 0.18 -0.3 1,733,643 - Psest_1633 0.18 -0.7 1,733,643 - Psest_1633 0.18 +0.6 1,733,643 - Psest_1633 0.18 +1.0 1,733,709 + Psest_1633 0.30 +0.3 1,733,710 - Psest_1633 0.30 +1.3 1,734,056 + -0.2 1,734,109 + +0.6 1,734,110 - -0.3 1,734,206 + +2.2 1,734,365 - +1.0 1,734,431 - Psest_1634 0.12 -0.8 1,734,431 - Psest_1634 0.12 -1.7 1,734,481 + Psest_1634 0.14 +2.6 1,734,560 - Psest_1634 0.18 +0.3 1,734,691 + Psest_1634 0.25 -1.2 1,734,691 + Psest_1634 0.25 +1.6 1,735,195 + Psest_1634 0.50 -2.0 1,735,255 + Psest_1634 0.53 +0.2 1,735,255 + Psest_1634 0.53 -0.0 1,735,255 + Psest_1634 0.53 -0.4 1,735,256 - Psest_1634 0.53 -0.9 1,735,256 - Psest_1634 0.53 +1.0 1,735,713 + Psest_1634 0.76 -2.4 1,735,713 + Psest_1634 0.76 -1.1 1,735,714 - Psest_1634 0.76 -1.4 1,735,714 - Psest_1634 0.76 -1.9 1,735,714 - Psest_1634 0.76 +0.3 1,735,714 - Psest_1634 0.76 -2.2 1,735,792 + Psest_1634 0.80 -0.7 1,735,792 + Psest_1634 0.80 -1.2 1,735,793 - Psest_1634 0.80 -1.4 1,735,793 - Psest_1634 0.80 -2.2 1,735,793 - Psest_1634 0.80 -1.9 1,735,793 - Psest_1634 0.80 +0.2 1,735,793 - Psest_1634 0.80 -2.2 1,736,089 + +1.7 1,736,089 + +1.4 1,736,089 + +0.7 1,736,090 - +0.6 1,736,090 - -0.7 1,736,253 + +2.1 1,736,253 + +0.3 1,736,270 + +0.3 1,736,654 + Psest_1635 0.15 -0.5 1,736,654 + Psest_1635 0.15 -0.6 1,736,654 + Psest_1635 0.15 +0.2 1,736,697 + Psest_1635 0.16 -0.0 1,736,697 + Psest_1635 0.16 +0.5 1,736,698 - Psest_1635 0.16 +0.8 1,736,698 - Psest_1635 0.16 +0.0 1,736,698 - Psest_1635 0.16 +0.3 1,736,698 - Psest_1635 0.16 -0.3 1,736,698 - Psest_1635 0.16 +0.1 1,737,040 - Psest_1635 0.29 -0.4 1,737,086 - Psest_1635 0.30 -0.8 1,737,086 - Psest_1635 0.30 -0.3 1,737,086 - Psest_1635 0.30 +0.1 1,737,086 - Psest_1635 0.30 +0.2 1,737,118 + Psest_1635 0.31 +1.3 1,737,118 + Psest_1635 0.31 +0.4 1,737,119 - Psest_1635 0.31 +0.0 1,737,119 - Psest_1635 0.31 -1.1 1,737,119 - Psest_1635 0.31 +0.8 1,737,120 + Psest_1635 0.32 +0.4 1,737,120 + Psest_1635 0.32 +0.4 1,737,120 + Psest_1635 0.32 +0.2 1,737,120 + Psest_1635 0.32 +1.0 1,737,120 + Psest_1635 0.32 +0.9 1,737,120 + Psest_1635 0.32 +0.5 1,737,120 + Psest_1635 0.32 +1.5 1,737,120 + Psest_1635 0.32 +0.8 1,737,120 + Psest_1635 0.32 +1.1 1,737,120 + Psest_1635 0.32 +0.5 1,737,121 - Psest_1635 0.32 +0.2 1,737,121 - Psest_1635 0.32 +0.9 1,737,121 - Psest_1635 0.32 +0.6 1,737,121 - Psest_1635 0.32 -0.0 1,737,121 - Psest_1635 0.32 +0.6 1,737,121 - Psest_1635 0.32 -0.2 1,737,121 - Psest_1635 0.32 +0.1 1,737,121 - Psest_1635 0.32 -0.2 1,737,121 - Psest_1635 0.32 +0.2 1,737,121 - Psest_1635 0.32 +0.2 1,737,121 - Psest_1635 0.32 +0.1
Or see this region's nucleotide sequence