Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0181 and Psest_0182 are separated by 52 nucleotides Psest_0182 and Psest_0183 are separated by 59 nucleotides Psest_0183 and Psest_0184 are separated by 183 nucleotides
Psest_0181: Psest_0181 - glycine cleavage system T protein, at 175,363 to 176,445
_0181
Psest_0182: Psest_0182 - glycine cleavage system H protein, at 176,498 to 176,887
_0182
Psest_0183: Psest_0183 - glycine dehydrogenase (decarboxylating), at 176,947 to 179,823
_0183
Psest_0184: Psest_0184 - ribosome biogenesis GTP-binding protein YsxC/EngB, at 180,007 to 180,660
_0184
Position (kb) 176
177
178
179
180 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 176.307 kb on + strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.308 kb on - strand, within Psest_0181 at 176.435 kb on + strand at 176.435 kb on + strand at 176.436 kb on - strand at 176.436 kb on - strand at 176.443 kb on + strand at 176.443 kb on + strand at 176.444 kb on - strand at 176.444 kb on - strand at 176.622 kb on - strand, within Psest_0182 at 176.622 kb on - strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.708 kb on + strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.709 kb on - strand, within Psest_0182 at 176.786 kb on + strand, within Psest_0182 at 176.940 kb on - strand at 176.940 kb on - strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.953 kb on + strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.954 kb on - strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 176.955 kb on + strand at 177.278 kb on - strand, within Psest_0183 at 177.278 kb on - strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.286 kb on + strand, within Psest_0183 at 177.287 kb on - strand, within Psest_0183 at 177.287 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.341 kb on - strand, within Psest_0183 at 177.803 kb on - strand, within Psest_0183 at 177.989 kb on - strand, within Psest_0183 at 177.989 kb on - strand, within Psest_0183 at 177.997 kb on + strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.577 kb on - strand, within Psest_0183 at 178.700 kb on - strand, within Psest_0183 at 178.900 kb on + strand, within Psest_0183 at 178.901 kb on - strand, within Psest_0183 at 178.901 kb on - strand, within Psest_0183 at 178.901 kb on - strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.921 kb on + strand, within Psest_0183 at 178.922 kb on - strand, within Psest_0183 at 178.922 kb on - strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.978 kb on + strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 178.979 kb on - strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.248 kb on + strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.249 kb on - strand, within Psest_0183 at 179.250 kb on + strand, within Psest_0183 at 179.311 kb on + strand, within Psest_0183 at 179.312 kb on - strand, within Psest_0183 at 179.312 kb on - strand, within Psest_0183 at 179.312 kb on - strand, within Psest_0183 at 179.312 kb on - strand, within Psest_0183 at 179.351 kb on - strand, within Psest_0183 at 179.458 kb on + strand, within Psest_0183 at 179.633 kb on + strand at 179.633 kb on + strand at 179.633 kb on + strand at 179.634 kb on - strand at 179.634 kb on - strand at 179.646 kb on + strand at 179.735 kb on - strand at 179.735 kb on - strand at 179.735 kb on - strand at 179.812 kb on + strand at 179.851 kb on + strand at 179.852 kb on - strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.959 kb on + strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 179.960 kb on - strand at 180.008 kb on + strand at 180.009 kb on - strand at 180.009 kb on - strand at 180.009 kb on - strand at 180.722 kb on - strand at 180.722 kb on - strand at 180.722 kb on - strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.723 kb on + strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.724 kb on - strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.725 kb on + strand at 180.726 kb on - strand at 180.726 kb on - strand at 180.726 kb on - strand at 180.741 kb on - strand at 180.773 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 176,307 + Psest_0181 0.87 -0.5 176,308 - Psest_0181 0.87 -3.8 176,308 - Psest_0181 0.87 +0.6 176,308 - Psest_0181 0.87 -2.1 176,308 - Psest_0181 0.87 +0.4 176,308 - Psest_0181 0.87 -1.9 176,435 + +0.1 176,435 + -1.5 176,436 - -1.5 176,436 - +0.3 176,443 + -1.4 176,443 + +0.4 176,444 - +0.4 176,444 - -0.7 176,622 - Psest_0182 0.32 -0.6 176,622 - Psest_0182 0.32 +1.7 176,708 + Psest_0182 0.54 -0.6 176,708 + Psest_0182 0.54 +0.5 176,708 + Psest_0182 0.54 +0.1 176,708 + Psest_0182 0.54 -0.6 176,708 + Psest_0182 0.54 +0.9 176,708 + Psest_0182 0.54 -1.3 176,709 - Psest_0182 0.54 +1.7 176,709 - Psest_0182 0.54 +0.8 176,709 - Psest_0182 0.54 +0.7 176,709 - Psest_0182 0.54 -0.9 176,709 - Psest_0182 0.54 +1.6 176,709 - Psest_0182 0.54 -0.8 176,709 - Psest_0182 0.54 +1.0 176,709 - Psest_0182 0.54 -0.7 176,709 - Psest_0182 0.54 -1.6 176,786 + Psest_0182 0.74 -1.4 176,940 - -1.1 176,940 - +0.4 176,953 + -2.5 176,953 + +0.1 176,953 + -0.8 176,953 + -2.6 176,953 + -1.6 176,953 + +0.7 176,953 + +1.1 176,953 + -0.7 176,954 - +1.2 176,954 - +1.6 176,954 - +1.8 176,954 - +1.4 176,954 - -1.3 176,954 - -1.6 176,954 - -0.8 176,954 - +0.8 176,955 + +1.4 176,955 + -0.7 176,955 + -1.8 176,955 + +0.3 176,955 + -0.4 177,278 - Psest_0183 0.12 -3.0 177,278 - Psest_0183 0.12 -2.9 177,286 + Psest_0183 0.12 -1.4 177,286 + Psest_0183 0.12 -1.5 177,286 + Psest_0183 0.12 +1.0 177,286 + Psest_0183 0.12 +2.3 177,287 - Psest_0183 0.12 -0.2 177,287 - Psest_0183 0.12 +1.9 177,341 - Psest_0183 0.14 -2.9 177,341 - Psest_0183 0.14 -1.5 177,341 - Psest_0183 0.14 -1.0 177,341 - Psest_0183 0.14 -1.4 177,803 - Psest_0183 0.30 +0.0 177,989 - Psest_0183 0.36 -1.5 177,989 - Psest_0183 0.36 -1.1 177,997 + Psest_0183 0.36 +1.0 178,577 - Psest_0183 0.57 -0.5 178,577 - Psest_0183 0.57 -0.9 178,577 - Psest_0183 0.57 +0.2 178,577 - Psest_0183 0.57 -0.2 178,577 - Psest_0183 0.57 -1.0 178,700 - Psest_0183 0.61 +1.9 178,900 + Psest_0183 0.68 +0.1 178,901 - Psest_0183 0.68 +0.6 178,901 - Psest_0183 0.68 -0.3 178,901 - Psest_0183 0.68 +0.6 178,921 + Psest_0183 0.69 -1.4 178,921 + Psest_0183 0.69 +1.7 178,921 + Psest_0183 0.69 -0.7 178,921 + Psest_0183 0.69 +0.1 178,921 + Psest_0183 0.69 -0.8 178,922 - Psest_0183 0.69 -1.6 178,922 - Psest_0183 0.69 +0.2 178,978 + Psest_0183 0.71 -0.4 178,978 + Psest_0183 0.71 +0.6 178,978 + Psest_0183 0.71 +2.4 178,978 + Psest_0183 0.71 +0.8 178,978 + Psest_0183 0.71 -3.0 178,978 + Psest_0183 0.71 -0.9 178,978 + Psest_0183 0.71 +0.5 178,978 + Psest_0183 0.71 +0.9 178,978 + Psest_0183 0.71 +0.1 178,979 - Psest_0183 0.71 +3.0 178,979 - Psest_0183 0.71 -1.4 178,979 - Psest_0183 0.71 -2.5 178,979 - Psest_0183 0.71 +0.9 178,979 - Psest_0183 0.71 +0.5 178,979 - Psest_0183 0.71 +2.4 178,979 - Psest_0183 0.71 -0.8 178,979 - Psest_0183 0.71 -2.2 178,979 - Psest_0183 0.71 -2.1 178,979 - Psest_0183 0.71 +1.3 179,248 + Psest_0183 0.80 -0.6 179,248 + Psest_0183 0.80 -0.5 179,248 + Psest_0183 0.80 -1.8 179,248 + Psest_0183 0.80 -0.8 179,248 + Psest_0183 0.80 -0.0 179,248 + Psest_0183 0.80 -0.7 179,248 + Psest_0183 0.80 -1.7 179,248 + Psest_0183 0.80 -1.4 179,248 + Psest_0183 0.80 -0.4 179,248 + Psest_0183 0.80 -0.7 179,248 + Psest_0183 0.80 -0.1 179,248 + Psest_0183 0.80 -1.5 179,248 + Psest_0183 0.80 +0.1 179,248 + Psest_0183 0.80 +0.5 179,248 + Psest_0183 0.80 -0.0 179,249 - Psest_0183 0.80 +0.6 179,249 - Psest_0183 0.80 -1.6 179,249 - Psest_0183 0.80 +2.7 179,249 - Psest_0183 0.80 +0.0 179,249 - Psest_0183 0.80 -0.2 179,249 - Psest_0183 0.80 +0.2 179,249 - Psest_0183 0.80 -1.5 179,249 - Psest_0183 0.80 +0.1 179,249 - Psest_0183 0.80 -0.4 179,249 - Psest_0183 0.80 -1.6 179,249 - Psest_0183 0.80 -0.3 179,249 - Psest_0183 0.80 +0.4 179,249 - Psest_0183 0.80 +0.2 179,249 - Psest_0183 0.80 -2.1 179,249 - Psest_0183 0.80 +0.6 179,250 + Psest_0183 0.80 -1.0 179,311 + Psest_0183 0.82 -1.5 179,312 - Psest_0183 0.82 +0.2 179,312 - Psest_0183 0.82 +0.2 179,312 - Psest_0183 0.82 -0.7 179,312 - Psest_0183 0.82 -1.0 179,351 - Psest_0183 0.84 +0.8 179,458 + Psest_0183 0.87 +0.2 179,633 + -1.7 179,633 + -1.6 179,633 + +0.8 179,634 - +1.4 179,634 - -1.5 179,646 + +1.4 179,735 - -0.4 179,735 - -1.0 179,735 - +0.1 179,812 + -1.6 179,851 + -0.3 179,852 - +0.1 179,959 + +0.2 179,959 + +0.4 179,959 + -1.1 179,959 + +1.7 179,959 + -0.2 179,959 + +0.9 179,959 + +1.3 179,959 + +1.6 179,959 + +2.9 179,959 + -0.3 179,959 + -1.0 179,959 + -1.4 179,959 + -1.2 179,959 + -2.1 179,959 + -0.2 179,959 + +0.5 179,959 + -0.1 179,960 - +2.1 179,960 - +2.4 179,960 - +1.3 179,960 - +0.2 179,960 - +1.6 179,960 - +0.4 179,960 - +0.4 179,960 - +1.4 179,960 - -0.2 179,960 - +0.1 179,960 - -1.4 180,008 + +2.9 180,009 - -0.1 180,009 - +0.5 180,009 - +3.5 180,722 - -0.9 180,722 - -1.6 180,722 - +0.0 180,723 + +0.0 180,723 + -0.1 180,723 + +1.5 180,723 + -0.2 180,723 + +1.3 180,723 + -0.3 180,723 + -0.2 180,723 + +0.3 180,723 + +0.6 180,723 + -1.3 180,723 + +0.5 180,723 + -0.5 180,723 + -1.9 180,723 + +0.0 180,724 - -1.7 180,724 - +0.5 180,724 - -1.9 180,724 - +2.8 180,724 - +0.1 180,724 - +2.1 180,724 - -1.0 180,724 - +2.7 180,724 - +0.7 180,724 - +0.7 180,724 - +1.9 180,724 - -0.4 180,724 - +0.6 180,725 + -1.2 180,725 + +1.4 180,725 + -0.1 180,725 + -1.1 180,725 + +1.4 180,725 + +2.5 180,725 + +1.7 180,725 + -1.9 180,725 + -0.4 180,725 + -0.1 180,725 + +0.9 180,725 + +0.9 180,725 + -0.2 180,726 - -1.0 180,726 - +0.9 180,726 - +1.1 180,741 - +0.6 180,773 + +0.4
Or see this region's nucleotide sequence