Experiment: Benzethonium chloride 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1329 and Psest_1330 overlap by 4 nucleotides Psest_1330 and Psest_1331 are separated by 241 nucleotides Psest_1331 and Psest_1332 are separated by 69 nucleotides Psest_1332 and Psest_1333 are separated by 220 nucleotides Psest_1333 and Psest_1334 are separated by 275 nucleotides
Psest_1329: Psest_1329 - Alkylated DNA repair protein, at 1,431,998 to 1,432,615
_1329
Psest_1330: Psest_1330 - hypothetical protein, at 1,432,612 to 1,432,773
_1330
Psest_1331: Psest_1331 - hypothetical protein, at 1,433,015 to 1,433,125
_1331
Psest_1332: Psest_1332 - alpha-1,6-glucosidases, pullulanase-type, at 1,433,195 to 1,436,314
_1332
Psest_1333: Psest_1333 - Acyl dehydratase, at 1,436,535 to 1,437,005
_1333
Psest_1334: Psest_1334 - Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II, at 1,437,281 to 1,438,969
_1334
Position (kb)
1433
1434
1435
1436
1437 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1432.217 kb on + strand, within Psest_1329 at 1432.477 kb on - strand, within Psest_1329 at 1432.477 kb on - strand, within Psest_1329 at 1432.477 kb on - strand, within Psest_1329 at 1432.799 kb on + strand at 1432.800 kb on - strand at 1432.800 kb on - strand at 1432.823 kb on + strand at 1432.824 kb on - strand at 1432.824 kb on - strand at 1432.867 kb on + strand at 1432.867 kb on + strand at 1432.867 kb on + strand at 1432.867 kb on + strand at 1432.870 kb on - strand at 1433.007 kb on + strand at 1433.027 kb on - strand, within Psest_1331 at 1433.159 kb on - strand at 1433.695 kb on - strand, within Psest_1332 at 1433.733 kb on + strand, within Psest_1332 at 1433.733 kb on + strand, within Psest_1332 at 1433.733 kb on + strand, within Psest_1332 at 1433.877 kb on + strand, within Psest_1332 at 1433.878 kb on - strand, within Psest_1332 at 1433.922 kb on + strand, within Psest_1332 at 1433.922 kb on + strand, within Psest_1332 at 1433.923 kb on - strand, within Psest_1332 at 1433.923 kb on - strand, within Psest_1332 at 1433.923 kb on - strand, within Psest_1332 at 1433.950 kb on + strand, within Psest_1332 at 1433.950 kb on + strand, within Psest_1332 at 1433.951 kb on - strand, within Psest_1332 at 1433.951 kb on - strand, within Psest_1332 at 1433.951 kb on - strand, within Psest_1332 at 1433.974 kb on - strand, within Psest_1332 at 1434.129 kb on + strand, within Psest_1332 at 1434.130 kb on - strand, within Psest_1332 at 1434.321 kb on + strand, within Psest_1332 at 1434.321 kb on + strand, within Psest_1332 at 1434.321 kb on + strand, within Psest_1332 at 1434.321 kb on + strand, within Psest_1332 at 1434.321 kb on + strand, within Psest_1332 at 1434.321 kb on + strand, within Psest_1332 at 1434.321 kb on + strand, within Psest_1332 at 1434.322 kb on - strand, within Psest_1332 at 1434.322 kb on - strand, within Psest_1332 at 1434.380 kb on - strand, within Psest_1332 at 1434.380 kb on - strand, within Psest_1332 at 1434.380 kb on - strand, within Psest_1332 at 1434.543 kb on + strand, within Psest_1332 at 1434.543 kb on + strand, within Psest_1332 at 1434.543 kb on + strand, within Psest_1332 at 1434.543 kb on + strand, within Psest_1332 at 1434.543 kb on + strand, within Psest_1332 at 1434.543 kb on + strand, within Psest_1332 at 1434.544 kb on - strand, within Psest_1332 at 1434.609 kb on + strand, within Psest_1332 at 1434.609 kb on + strand, within Psest_1332 at 1434.609 kb on + strand, within Psest_1332 at 1434.745 kb on - strand, within Psest_1332 at 1434.784 kb on - strand, within Psest_1332 at 1434.855 kb on + strand, within Psest_1332 at 1435.056 kb on + strand, within Psest_1332 at 1435.057 kb on - strand, within Psest_1332 at 1435.057 kb on - strand, within Psest_1332 at 1435.222 kb on - strand, within Psest_1332 at 1435.321 kb on - strand, within Psest_1332 at 1435.323 kb on + strand, within Psest_1332 at 1435.323 kb on + strand, within Psest_1332 at 1435.323 kb on + strand, within Psest_1332 at 1435.324 kb on - strand, within Psest_1332 at 1435.381 kb on + strand, within Psest_1332 at 1435.512 kb on + strand, within Psest_1332 at 1435.512 kb on + strand, within Psest_1332 at 1435.512 kb on + strand, within Psest_1332 at 1435.512 kb on + strand, within Psest_1332 at 1435.512 kb on + strand, within Psest_1332 at 1435.513 kb on - strand, within Psest_1332 at 1435.584 kb on + strand, within Psest_1332 at 1435.650 kb on + strand, within Psest_1332 at 1435.744 kb on + strand, within Psest_1332 at 1435.745 kb on - strand, within Psest_1332 at 1435.786 kb on - strand, within Psest_1332 at 1436.053 kb on - strand at 1436.053 kb on - strand at 1436.053 kb on - strand at 1436.169 kb on + strand at 1436.169 kb on + strand at 1436.193 kb on + strand at 1436.194 kb on - strand at 1436.194 kb on - strand at 1436.342 kb on + strand at 1436.342 kb on + strand at 1436.342 kb on + strand at 1436.342 kb on + strand at 1436.342 kb on + strand at 1436.343 kb on - strand at 1436.343 kb on - strand at 1436.343 kb on - strand at 1436.411 kb on - strand at 1436.987 kb on + strand at 1437.076 kb on + strand at 1437.109 kb on + strand at 1437.110 kb on - strand at 1437.220 kb on + strand at 1437.221 kb on - strand at 1437.265 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Benzethonium chloride 0.0125 mM remove 1,432,217 + Psest_1329 0.35 +2.9 1,432,477 - Psest_1329 0.78 -0.4 1,432,477 - Psest_1329 0.78 -1.8 1,432,477 - Psest_1329 0.78 -2.3 1,432,799 + -1.1 1,432,800 - +0.9 1,432,800 - +0.2 1,432,823 + +0.0 1,432,824 - -0.0 1,432,824 - +1.4 1,432,867 + +0.6 1,432,867 + +0.8 1,432,867 + +0.6 1,432,867 + -0.8 1,432,870 - +1.1 1,433,007 + -0.6 1,433,027 - Psest_1331 0.11 +0.2 1,433,159 - -1.1 1,433,695 - Psest_1332 0.16 +0.7 1,433,733 + Psest_1332 0.17 +0.2 1,433,733 + Psest_1332 0.17 +2.4 1,433,733 + Psest_1332 0.17 +0.4 1,433,877 + Psest_1332 0.22 -1.2 1,433,878 - Psest_1332 0.22 +0.0 1,433,922 + Psest_1332 0.23 +2.2 1,433,922 + Psest_1332 0.23 +0.0 1,433,923 - Psest_1332 0.23 -2.0 1,433,923 - Psest_1332 0.23 -0.7 1,433,923 - Psest_1332 0.23 +2.4 1,433,950 + Psest_1332 0.24 +1.8 1,433,950 + Psest_1332 0.24 +1.6 1,433,951 - Psest_1332 0.24 +0.2 1,433,951 - Psest_1332 0.24 -0.1 1,433,951 - Psest_1332 0.24 -1.2 1,433,974 - Psest_1332 0.25 +1.2 1,434,129 + Psest_1332 0.30 -2.8 1,434,130 - Psest_1332 0.30 +1.4 1,434,321 + Psest_1332 0.36 -0.1 1,434,321 + Psest_1332 0.36 +0.7 1,434,321 + Psest_1332 0.36 +0.6 1,434,321 + Psest_1332 0.36 +0.5 1,434,321 + Psest_1332 0.36 -0.1 1,434,321 + Psest_1332 0.36 +0.1 1,434,321 + Psest_1332 0.36 -0.2 1,434,322 - Psest_1332 0.36 -0.3 1,434,322 - Psest_1332 0.36 -0.6 1,434,380 - Psest_1332 0.38 +0.1 1,434,380 - Psest_1332 0.38 +0.7 1,434,380 - Psest_1332 0.38 -0.8 1,434,543 + Psest_1332 0.43 -1.4 1,434,543 + Psest_1332 0.43 +0.1 1,434,543 + Psest_1332 0.43 -0.1 1,434,543 + Psest_1332 0.43 -0.5 1,434,543 + Psest_1332 0.43 -0.5 1,434,543 + Psest_1332 0.43 +1.2 1,434,544 - Psest_1332 0.43 -1.1 1,434,609 + Psest_1332 0.45 +0.7 1,434,609 + Psest_1332 0.45 +1.3 1,434,609 + Psest_1332 0.45 -2.5 1,434,745 - Psest_1332 0.50 +0.5 1,434,784 - Psest_1332 0.51 +0.5 1,434,855 + Psest_1332 0.53 +0.5 1,435,056 + Psest_1332 0.60 +1.2 1,435,057 - Psest_1332 0.60 -0.0 1,435,057 - Psest_1332 0.60 +0.7 1,435,222 - Psest_1332 0.65 -1.6 1,435,321 - Psest_1332 0.68 -1.1 1,435,323 + Psest_1332 0.68 +2.0 1,435,323 + Psest_1332 0.68 -0.0 1,435,323 + Psest_1332 0.68 +0.2 1,435,324 - Psest_1332 0.68 +0.5 1,435,381 + Psest_1332 0.70 +0.5 1,435,512 + Psest_1332 0.74 +1.0 1,435,512 + Psest_1332 0.74 -1.1 1,435,512 + Psest_1332 0.74 +0.5 1,435,512 + Psest_1332 0.74 -1.2 1,435,512 + Psest_1332 0.74 +0.0 1,435,513 - Psest_1332 0.74 -0.4 1,435,584 + Psest_1332 0.77 +0.9 1,435,650 + Psest_1332 0.79 -1.9 1,435,744 + Psest_1332 0.82 -0.1 1,435,745 - Psest_1332 0.82 +0.5 1,435,786 - Psest_1332 0.83 +0.3 1,436,053 - +0.0 1,436,053 - -1.0 1,436,053 - +0.1 1,436,169 + -0.2 1,436,169 + -0.7 1,436,193 + -1.2 1,436,194 - -0.7 1,436,194 - -1.2 1,436,342 + -0.1 1,436,342 + +1.8 1,436,342 + -0.3 1,436,342 + +2.3 1,436,342 + +0.7 1,436,343 - -0.5 1,436,343 - +0.5 1,436,343 - -1.4 1,436,411 - +0.2 1,436,987 + +0.4 1,437,076 + -1.9 1,437,109 + +0.2 1,437,110 - -1.4 1,437,220 + +0.1 1,437,221 - +0.1 1,437,265 - -0.8
Or see this region's nucleotide sequence