Experiment: 5-Hydroxymethylfurfural 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1002 and Psest_1003 are separated by 89 nucleotides Psest_1003 and Psest_1004 are separated by 191 nucleotides Psest_1004 and Psest_1005 overlap by 1 nucleotides Psest_1005 and Psest_1006 are separated by 263 nucleotides
Psest_1002: Psest_1002 - glutamyl-queuosine tRNA(Asp) synthetase, at 1,087,302 to 1,088,207
_1002
Psest_1003: Psest_1003 - RNA polymerase-binding protein DksA, at 1,088,297 to 1,088,743
_1003
Psest_1004: Psest_1004 - Aspartate/tyrosine/aromatic aminotransferase, at 1,088,935 to 1,090,107
_1004
Psest_1005: Psest_1005 - sugar fermentation stimulation protein, at 1,090,107 to 1,090,814
_1005
Psest_1006: Psest_1006 - dTDP-4-dehydrorhamnose reductase, at 1,091,078 to 1,091,998
_1006
Position (kb)
1088
1089
1090
1091 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1087.990 kb on + strand, within Psest_1002 at 1087.991 kb on - strand, within Psest_1002 at 1087.991 kb on - strand, within Psest_1002 at 1088.101 kb on + strand, within Psest_1002 at 1088.102 kb on - strand, within Psest_1002 at 1088.102 kb on - strand, within Psest_1002 at 1088.686 kb on - strand, within Psest_1003 at 1088.686 kb on - strand, within Psest_1003 at 1088.901 kb on - strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.947 kb on + strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1088.948 kb on - strand at 1089.128 kb on - strand, within Psest_1004 at 1089.130 kb on - strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.197 kb on + strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.198 kb on - strand, within Psest_1004 at 1089.202 kb on - strand, within Psest_1004 at 1089.202 kb on - strand, within Psest_1004 at 1089.553 kb on + strand, within Psest_1004 at 1089.553 kb on + strand, within Psest_1004 at 1089.553 kb on + strand, within Psest_1004 at 1089.553 kb on + strand, within Psest_1004 at 1089.554 kb on - strand, within Psest_1004 at 1089.554 kb on - strand, within Psest_1004 at 1089.554 kb on - strand, within Psest_1004 at 1089.554 kb on - strand, within Psest_1004 at 1089.569 kb on - strand, within Psest_1004 at 1089.600 kb on - strand, within Psest_1004 at 1089.637 kb on + strand, within Psest_1004 at 1089.637 kb on + strand, within Psest_1004 at 1089.637 kb on + strand, within Psest_1004 at 1089.638 kb on - strand, within Psest_1004 at 1089.638 kb on - strand, within Psest_1004 at 1089.638 kb on - strand, within Psest_1004 at 1089.724 kb on + strand, within Psest_1004 at 1089.725 kb on - strand, within Psest_1004 at 1089.725 kb on - strand, within Psest_1004 at 1089.867 kb on + strand, within Psest_1004 at 1089.896 kb on - strand, within Psest_1004 at 1089.896 kb on - strand, within Psest_1004 at 1089.896 kb on - strand, within Psest_1004 at 1089.902 kb on - strand, within Psest_1004 at 1089.902 kb on - strand, within Psest_1004 at 1090.034 kb on - strand at 1090.034 kb on - strand at 1090.152 kb on + strand at 1090.443 kb on + strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.444 kb on - strand, within Psest_1005 at 1090.642 kb on - strand, within Psest_1005 at 1090.642 kb on - strand, within Psest_1005 at 1090.699 kb on + strand, within Psest_1005 at 1090.699 kb on + strand, within Psest_1005 at 1090.700 kb on - strand, within Psest_1005 at 1090.700 kb on - strand, within Psest_1005 at 1090.701 kb on + strand, within Psest_1005 at 1090.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 5 mM remove 1,087,990 + Psest_1002 0.76 -2.9 1,087,991 - Psest_1002 0.76 -1.9 1,087,991 - Psest_1002 0.76 -0.7 1,088,101 + Psest_1002 0.88 +1.0 1,088,102 - Psest_1002 0.88 -0.6 1,088,102 - Psest_1002 0.88 +1.6 1,088,686 - Psest_1003 0.87 -1.1 1,088,686 - Psest_1003 0.87 -1.1 1,088,901 - -0.7 1,088,947 + -2.2 1,088,947 + -1.0 1,088,947 + -0.2 1,088,947 + -3.3 1,088,947 + -0.5 1,088,947 + -0.9 1,088,947 + -1.4 1,088,947 + -1.9 1,088,948 - +0.2 1,088,948 - +3.1 1,088,948 - +1.0 1,088,948 - -0.4 1,089,128 - Psest_1004 0.16 +1.7 1,089,130 - Psest_1004 0.17 +1.2 1,089,197 + Psest_1004 0.22 +1.4 1,089,197 + Psest_1004 0.22 -1.1 1,089,197 + Psest_1004 0.22 +1.2 1,089,198 - Psest_1004 0.22 -0.7 1,089,198 - Psest_1004 0.22 +0.2 1,089,198 - Psest_1004 0.22 +2.0 1,089,198 - Psest_1004 0.22 +1.9 1,089,202 - Psest_1004 0.23 -0.3 1,089,202 - Psest_1004 0.23 +3.4 1,089,553 + Psest_1004 0.53 -1.1 1,089,553 + Psest_1004 0.53 +0.1 1,089,553 + Psest_1004 0.53 +0.1 1,089,553 + Psest_1004 0.53 -0.4 1,089,554 - Psest_1004 0.53 +2.2 1,089,554 - Psest_1004 0.53 +0.0 1,089,554 - Psest_1004 0.53 +4.6 1,089,554 - Psest_1004 0.53 +0.2 1,089,569 - Psest_1004 0.54 -0.4 1,089,600 - Psest_1004 0.57 +0.9 1,089,637 + Psest_1004 0.60 -0.3 1,089,637 + Psest_1004 0.60 -1.1 1,089,637 + Psest_1004 0.60 +0.6 1,089,638 - Psest_1004 0.60 +2.7 1,089,638 - Psest_1004 0.60 +2.4 1,089,638 - Psest_1004 0.60 -2.1 1,089,724 + Psest_1004 0.67 +1.0 1,089,725 - Psest_1004 0.67 -0.0 1,089,725 - Psest_1004 0.67 -1.2 1,089,867 + Psest_1004 0.79 -0.9 1,089,896 - Psest_1004 0.82 +0.3 1,089,896 - Psest_1004 0.82 +1.1 1,089,896 - Psest_1004 0.82 +1.1 1,089,902 - Psest_1004 0.82 +0.0 1,089,902 - Psest_1004 0.82 -1.6 1,090,034 - -0.9 1,090,034 - +0.7 1,090,152 + +1.3 1,090,443 + Psest_1005 0.47 -1.4 1,090,444 - Psest_1005 0.48 +0.0 1,090,444 - Psest_1005 0.48 -1.0 1,090,444 - Psest_1005 0.48 +0.4 1,090,444 - Psest_1005 0.48 +1.0 1,090,444 - Psest_1005 0.48 -1.2 1,090,642 - Psest_1005 0.76 -1.5 1,090,642 - Psest_1005 0.76 -1.0 1,090,699 + Psest_1005 0.84 +0.9 1,090,699 + Psest_1005 0.84 -1.6 1,090,700 - Psest_1005 0.84 -0.9 1,090,700 - Psest_1005 0.84 +0.2 1,090,701 + Psest_1005 0.84 +1.2 1,090,943 - +1.9
Or see this region's nucleotide sequence