Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0051

Experiment: 5-Hydroxymethylfurfural 5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0049 and Psest_0050 are separated by 155 nucleotidesPsest_0050 and Psest_0051 are separated by 193 nucleotidesPsest_0051 and Psest_0052 are separated by 10 nucleotidesPsest_0052 and Psest_0053 are separated by 167 nucleotides Psest_0049: Psest_0049 - Uncharacterized conserved protein, at 52,951 to 53,556 _0049 Psest_0050: Psest_0050 - Predicted acetyltransferase, at 53,712 to 53,993 _0050 Psest_0051: Psest_0051 - hypothetical protein, at 54,187 to 54,516 _0051 Psest_0052: Psest_0052 - hypothetical protein, at 54,527 to 55,279 _0052 Psest_0053: Psest_0053 - hypothetical protein, at 55,447 to 55,950 _0053 Position (kb) 54 55Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 53.211 kb on + strand, within Psest_0049at 53.211 kb on + strand, within Psest_0049at 53.211 kb on + strand, within Psest_0049at 53.211 kb on + strand, within Psest_0049at 53.211 kb on + strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.212 kb on - strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.284 kb on + strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.285 kb on - strand, within Psest_0049at 53.286 kb on + strand, within Psest_0049at 53.286 kb on + strand, within Psest_0049at 53.286 kb on + strand, within Psest_0049at 53.287 kb on - strand, within Psest_0049at 53.287 kb on - strand, within Psest_0049at 53.287 kb on - strand, within Psest_0049at 53.287 kb on - strand, within Psest_0049at 53.287 kb on - strand, within Psest_0049at 53.361 kb on + strand, within Psest_0049at 53.362 kb on - strand, within Psest_0049at 53.362 kb on - strand, within Psest_0049at 53.596 kb on - strandat 53.659 kb on + strandat 53.659 kb on + strandat 53.659 kb on + strandat 53.660 kb on - strandat 53.660 kb on - strandat 53.676 kb on + strandat 53.676 kb on + strandat 53.676 kb on + strandat 53.677 kb on - strandat 53.761 kb on - strand, within Psest_0050at 53.946 kb on + strand, within Psest_0050at 53.946 kb on + strand, within Psest_0050at 53.946 kb on + strand, within Psest_0050at 53.946 kb on + strand, within Psest_0050at 53.946 kb on + strand, within Psest_0050at 53.947 kb on - strand, within Psest_0050at 53.947 kb on - strand, within Psest_0050at 53.947 kb on - strand, within Psest_0050at 53.947 kb on - strand, within Psest_0050at 53.947 kb on - strand, within Psest_0050at 53.999 kb on - strandat 54.019 kb on + strandat 54.019 kb on + strandat 54.019 kb on + strandat 54.019 kb on + strandat 54.019 kb on + strandat 54.019 kb on + strandat 54.020 kb on - strandat 54.040 kb on + strandat 54.041 kb on - strandat 54.134 kb on - strandat 54.218 kb on + strandat 54.219 kb on - strandat 54.276 kb on + strand, within Psest_0051at 54.276 kb on + strand, within Psest_0051at 54.277 kb on - strand, within Psest_0051at 54.277 kb on - strand, within Psest_0051at 54.277 kb on - strand, within Psest_0051at 54.277 kb on - strand, within Psest_0051at 54.433 kb on + strand, within Psest_0051at 54.433 kb on + strand, within Psest_0051at 54.433 kb on + strand, within Psest_0051at 54.433 kb on + strand, within Psest_0051at 54.434 kb on - strand, within Psest_0051at 54.434 kb on - strand, within Psest_0051at 54.434 kb on - strand, within Psest_0051at 54.488 kb on + strandat 54.488 kb on + strandat 54.488 kb on + strandat 54.488 kb on + strandat 54.488 kb on + strandat 54.489 kb on - strandat 54.489 kb on - strandat 54.735 kb on + strand, within Psest_0052at 54.736 kb on - strand, within Psest_0052at 54.736 kb on - strand, within Psest_0052at 54.736 kb on - strand, within Psest_0052at 54.736 kb on - strand, within Psest_0052at 54.736 kb on - strand, within Psest_0052at 54.780 kb on + strand, within Psest_0052at 54.803 kb on + strand, within Psest_0052at 54.825 kb on + strand, within Psest_0052at 54.825 kb on + strand, within Psest_0052at 54.825 kb on + strand, within Psest_0052at 54.831 kb on + strand, within Psest_0052at 54.831 kb on + strand, within Psest_0052at 54.831 kb on + strand, within Psest_0052at 54.831 kb on + strand, within Psest_0052at 54.832 kb on - strand, within Psest_0052at 54.832 kb on - strand, within Psest_0052at 54.832 kb on - strand, within Psest_0052at 54.832 kb on - strand, within Psest_0052at 54.862 kb on - strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 54.999 kb on + strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.000 kb on - strand, within Psest_0052at 55.034 kb on + strand, within Psest_0052at 55.065 kb on + strand, within Psest_0052at 55.065 kb on + strand, within Psest_0052at 55.066 kb on - strand, within Psest_0052at 55.066 kb on - strand, within Psest_0052at 55.168 kb on - strand, within Psest_0052at 55.199 kb on + strand, within Psest_0052at 55.200 kb on - strand, within Psest_0052at 55.200 kb on - strand, within Psest_0052at 55.264 kb on - strandat 55.306 kb on + strandat 55.313 kb on - strandat 55.313 kb on - strandat 55.421 kb on - strandat 55.424 kb on - strandat 55.424 kb on - strandat 55.424 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 5-Hydroxymethylfurfural 5 mM
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53,211 + Psest_0049 0.43 +0.3
53,211 + Psest_0049 0.43 -0.4
53,211 + Psest_0049 0.43 -1.7
53,211 + Psest_0049 0.43 +1.7
53,211 + Psest_0049 0.43 +0.4
53,212 - Psest_0049 0.43 -0.8
53,212 - Psest_0049 0.43 -1.9
53,212 - Psest_0049 0.43 -0.9
53,212 - Psest_0049 0.43 -0.3
53,212 - Psest_0049 0.43 -1.9
53,212 - Psest_0049 0.43 +0.2
53,212 - Psest_0049 0.43 +0.6
53,212 - Psest_0049 0.43 +0.7
53,212 - Psest_0049 0.43 +0.1
53,212 - Psest_0049 0.43 -1.6
53,284 + Psest_0049 0.55 -0.9
53,284 + Psest_0049 0.55 +1.3
53,284 + Psest_0049 0.55 -2.4
53,284 + Psest_0049 0.55 -1.0
53,284 + Psest_0049 0.55 +0.6
53,284 + Psest_0049 0.55 +0.4
53,284 + Psest_0049 0.55 +0.2
53,284 + Psest_0049 0.55 -1.7
53,284 + Psest_0049 0.55 -0.6
53,284 + Psest_0049 0.55 +0.5
53,285 - Psest_0049 0.55 -0.6
53,285 - Psest_0049 0.55 +1.9
53,285 - Psest_0049 0.55 +1.9
53,285 - Psest_0049 0.55 -1.8
53,285 - Psest_0049 0.55 -0.1
53,285 - Psest_0049 0.55 +0.1
53,285 - Psest_0049 0.55 -1.1
53,285 - Psest_0049 0.55 -2.7
53,285 - Psest_0049 0.55 +1.6
53,285 - Psest_0049 0.55 +1.4
53,285 - Psest_0049 0.55 -0.1
53,285 - Psest_0049 0.55 -1.3
53,285 - Psest_0049 0.55 -0.5
53,286 + Psest_0049 0.55 +2.3
53,286 + Psest_0049 0.55 +1.0
53,286 + Psest_0049 0.55 -0.6
53,287 - Psest_0049 0.55 +0.7
53,287 - Psest_0049 0.55 +0.8
53,287 - Psest_0049 0.55 +0.8
53,287 - Psest_0049 0.55 +0.3
53,287 - Psest_0049 0.55 -3.2
53,361 + Psest_0049 0.68 -1.2
53,362 - Psest_0049 0.68 -0.2
53,362 - Psest_0049 0.68 -0.5
53,596 - -1.6
53,659 + -0.9
53,659 + -0.7
53,659 + -0.5
53,660 - -0.0
53,660 - +4.1
53,676 + +0.7
53,676 + -0.3
53,676 + +1.3
53,677 - +2.1
53,761 - Psest_0050 0.17 +0.9
53,946 + Psest_0050 0.83 -2.1
53,946 + Psest_0050 0.83 +2.0
53,946 + Psest_0050 0.83 -0.8
53,946 + Psest_0050 0.83 +1.4
53,946 + Psest_0050 0.83 -1.7
53,947 - Psest_0050 0.83 -0.7
53,947 - Psest_0050 0.83 -0.2
53,947 - Psest_0050 0.83 +1.1
53,947 - Psest_0050 0.83 -0.3
53,947 - Psest_0050 0.83 +2.5
53,999 - +1.5
54,019 + +0.2
54,019 + +1.1
54,019 + -1.6
54,019 + -0.4
54,019 + -1.9
54,019 + -0.2
54,020 - +0.8
54,040 + +0.5
54,041 - -0.3
54,134 - -3.2
54,218 + -0.5
54,219 - +0.7
54,276 + Psest_0051 0.27 +0.0
54,276 + Psest_0051 0.27 -2.6
54,277 - Psest_0051 0.27 -0.9
54,277 - Psest_0051 0.27 -1.9
54,277 - Psest_0051 0.27 -0.8
54,277 - Psest_0051 0.27 +0.7
54,433 + Psest_0051 0.75 -0.2
54,433 + Psest_0051 0.75 +0.2
54,433 + Psest_0051 0.75 -0.7
54,433 + Psest_0051 0.75 -1.5
54,434 - Psest_0051 0.75 -1.6
54,434 - Psest_0051 0.75 -0.7
54,434 - Psest_0051 0.75 -1.8
54,488 + +0.3
54,488 + +1.2
54,488 + -0.6
54,488 + -1.3
54,488 + +1.0
54,489 - -0.0
54,489 - -0.7
54,735 + Psest_0052 0.28 -1.5
54,736 - Psest_0052 0.28 +0.0
54,736 - Psest_0052 0.28 +1.5
54,736 - Psest_0052 0.28 -1.3
54,736 - Psest_0052 0.28 +3.1
54,736 - Psest_0052 0.28 +1.2
54,780 + Psest_0052 0.34 +0.2
54,803 + Psest_0052 0.37 -1.3
54,825 + Psest_0052 0.40 -1.5
54,825 + Psest_0052 0.40 +0.4
54,825 + Psest_0052 0.40 +0.3
54,831 + Psest_0052 0.40 +0.2
54,831 + Psest_0052 0.40 -0.2
54,831 + Psest_0052 0.40 +0.8
54,831 + Psest_0052 0.40 -0.1
54,832 - Psest_0052 0.41 +0.9
54,832 - Psest_0052 0.41 +1.1
54,832 - Psest_0052 0.41 -1.7
54,832 - Psest_0052 0.41 -0.7
54,862 - Psest_0052 0.44 +0.4
54,999 + Psest_0052 0.63 -1.9
54,999 + Psest_0052 0.63 -1.0
54,999 + Psest_0052 0.63 -0.3
54,999 + Psest_0052 0.63 -0.1
54,999 + Psest_0052 0.63 -1.5
54,999 + Psest_0052 0.63 +1.4
54,999 + Psest_0052 0.63 -2.8
54,999 + Psest_0052 0.63 +0.7
54,999 + Psest_0052 0.63 +0.1
54,999 + Psest_0052 0.63 -0.1
55,000 - Psest_0052 0.63 -1.5
55,000 - Psest_0052 0.63 -2.2
55,000 - Psest_0052 0.63 -0.6
55,000 - Psest_0052 0.63 -0.9
55,000 - Psest_0052 0.63 -1.3
55,000 - Psest_0052 0.63 +1.1
55,000 - Psest_0052 0.63 -0.1
55,000 - Psest_0052 0.63 -2.6
55,000 - Psest_0052 0.63 +2.8
55,000 - Psest_0052 0.63 +1.3
55,034 + Psest_0052 0.67 +2.3
55,065 + Psest_0052 0.71 -0.5
55,065 + Psest_0052 0.71 -0.5
55,066 - Psest_0052 0.72 -1.0
55,066 - Psest_0052 0.72 +0.1
55,168 - Psest_0052 0.85 +1.3
55,199 + Psest_0052 0.89 +0.5
55,200 - Psest_0052 0.89 +0.7
55,200 - Psest_0052 0.89 +0.9
55,264 - -1.4
55,306 + +1.5
55,313 - -1.8
55,313 - -2.4
55,421 - +0.4
55,424 - -0.7
55,424 - -0.9
55,424 - +1.1

Or see this region's nucleotide sequence