Experiment: D-Mannose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05365 and HMPREF1058_RS21500 are separated by 212 nucleotides HMPREF1058_RS21500 and HMPREF1058_RS05360 are separated by 32 nucleotides HMPREF1058_RS05360 and HMPREF1058_RS05355 are separated by 11 nucleotides HMPREF1058_RS05355 and HMPREF1058_RS05350 are separated by 188 nucleotides
HMPREF1058_RS05365: HMPREF1058_RS05365 - hypothetical protein, at 181,794 to 182,354
_RS05365
HMPREF1058_RS21500: HMPREF1058_RS21500 - hypothetical protein, at 182,567 to 182,740
_RS21500
HMPREF1058_RS05360: HMPREF1058_RS05360 - helix-turn-helix domain-containing protein, at 182,773 to 183,099
_RS05360
HMPREF1058_RS05355: HMPREF1058_RS05355 - helix-turn-helix domain-containing protein, at 183,111 to 183,431
_RS05355
HMPREF1058_RS05350: HMPREF1058_RS05350 - Fic family protein, at 183,620 to 184,642
_RS05350
Position (kb) 182
183
184 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 181.921 kb on - strand, within HMPREF1058_RS05365 at 182.011 kb on - strand, within HMPREF1058_RS05365 at 182.011 kb on - strand, within HMPREF1058_RS05365 at 182.179 kb on + strand, within HMPREF1058_RS05365 at 182.305 kb on + strand at 182.306 kb on - strand at 182.368 kb on + strand at 182.368 kb on + strand at 182.549 kb on + strand at 182.550 kb on - strand at 182.780 kb on + strand at 182.781 kb on - strand at 182.997 kb on - strand, within HMPREF1058_RS05360 at 183.054 kb on + strand, within HMPREF1058_RS05360 at 183.086 kb on + strand at 183.087 kb on - strand at 183.098 kb on - strand at 183.111 kb on - strand at 183.215 kb on - strand, within HMPREF1058_RS05355 at 183.252 kb on + strand, within HMPREF1058_RS05355 at 183.255 kb on + strand, within HMPREF1058_RS05355 at 183.255 kb on + strand, within HMPREF1058_RS05355 at 183.256 kb on - strand, within HMPREF1058_RS05355 at 183.347 kb on - strand, within HMPREF1058_RS05355 at 183.387 kb on - strand, within HMPREF1058_RS05355 at 183.387 kb on - strand, within HMPREF1058_RS05355 at 183.402 kb on + strand at 183.403 kb on - strand at 183.441 kb on + strand at 183.477 kb on - strand at 183.577 kb on - strand at 183.646 kb on - strand at 183.699 kb on - strand at 183.724 kb on + strand, within HMPREF1058_RS05350 at 183.945 kb on - strand, within HMPREF1058_RS05350 at 184.028 kb on + strand, within HMPREF1058_RS05350 at 184.028 kb on + strand, within HMPREF1058_RS05350
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose 10 mM (C) remove 181,921 - HMPREF1058_RS05365 0.23 -3.2 182,011 - HMPREF1058_RS05365 0.39 -0.7 182,011 - HMPREF1058_RS05365 0.39 -0.3 182,179 + HMPREF1058_RS05365 0.69 -0.5 182,305 + -1.8 182,306 - -0.4 182,368 + -1.5 182,368 + -0.5 182,549 + -0.7 182,550 - -1.0 182,780 + -0.6 182,781 - -0.5 182,997 - HMPREF1058_RS05360 0.69 -0.4 183,054 + HMPREF1058_RS05360 0.86 -2.4 183,086 + +0.1 183,087 - +1.4 183,098 - +0.8 183,111 - -0.1 183,215 - HMPREF1058_RS05355 0.32 +1.9 183,252 + HMPREF1058_RS05355 0.44 -0.5 183,255 + HMPREF1058_RS05355 0.45 +1.6 183,255 + HMPREF1058_RS05355 0.45 -2.9 183,256 - HMPREF1058_RS05355 0.45 -1.3 183,347 - HMPREF1058_RS05355 0.74 -0.2 183,387 - HMPREF1058_RS05355 0.86 -0.7 183,387 - HMPREF1058_RS05355 0.86 +2.6 183,402 + -0.5 183,403 - +0.7 183,441 + +0.0 183,477 - -2.3 183,577 - +0.6 183,646 - -0.0 183,699 - +0.1 183,724 + HMPREF1058_RS05350 0.10 +0.1 183,945 - HMPREF1058_RS05350 0.32 -0.4 184,028 + HMPREF1058_RS05350 0.40 -2.4 184,028 + HMPREF1058_RS05350 0.40 -1.9
Or see this region's nucleotide sequence