Experiment: D-Mannose 10 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS01660 and HMPREF1058_RS01655 are separated by 3 nucleotides HMPREF1058_RS01655 and HMPREF1058_RS01650 are separated by 15 nucleotides HMPREF1058_RS01650 and HMPREF1058_RS01645 are separated by 71 nucleotides HMPREF1058_RS01645 and HMPREF1058_RS01640 are separated by 101 nucleotides
HMPREF1058_RS01660: HMPREF1058_RS01660 - lipoprotein signal peptidase, at 74,486 to 75,124
_RS01660
HMPREF1058_RS01655: HMPREF1058_RS01655 - DUF4296 domain-containing protein, at 75,128 to 76,102
_RS01655
HMPREF1058_RS01650: HMPREF1058_RS01650 - hypothetical protein, at 76,118 to 76,441
_RS01650
HMPREF1058_RS01645: HMPREF1058_RS01645 - RNA methyltransferase, at 76,513 to 77,268
_RS01645
HMPREF1058_RS01640: HMPREF1058_RS01640 - BamA/TamA family outer membrane protein, at 77,370 to 79,694
_RS01640
Position (kb) 76
77 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 75.519 kb on + strand, within HMPREF1058_RS01655 at 75.519 kb on + strand, within HMPREF1058_RS01655 at 75.520 kb on - strand, within HMPREF1058_RS01655 at 75.524 kb on + strand, within HMPREF1058_RS01655 at 75.638 kb on + strand, within HMPREF1058_RS01655 at 75.639 kb on - strand, within HMPREF1058_RS01655 at 75.640 kb on + strand, within HMPREF1058_RS01655 at 75.640 kb on + strand, within HMPREF1058_RS01655 at 75.641 kb on - strand, within HMPREF1058_RS01655 at 75.641 kb on - strand, within HMPREF1058_RS01655 at 75.643 kb on - strand, within HMPREF1058_RS01655 at 75.643 kb on - strand, within HMPREF1058_RS01655 at 75.756 kb on - strand, within HMPREF1058_RS01655 at 75.757 kb on + strand, within HMPREF1058_RS01655 at 75.760 kb on - strand, within HMPREF1058_RS01655 at 75.809 kb on + strand, within HMPREF1058_RS01655 at 75.809 kb on + strand, within HMPREF1058_RS01655 at 75.810 kb on - strand, within HMPREF1058_RS01655 at 75.811 kb on + strand, within HMPREF1058_RS01655 at 75.811 kb on + strand, within HMPREF1058_RS01655 at 75.893 kb on - strand, within HMPREF1058_RS01655 at 76.146 kb on - strand at 76.171 kb on + strand, within HMPREF1058_RS01650 at 76.175 kb on + strand, within HMPREF1058_RS01650 at 76.175 kb on + strand, within HMPREF1058_RS01650 at 76.176 kb on - strand, within HMPREF1058_RS01650 at 76.176 kb on - strand, within HMPREF1058_RS01650 at 76.365 kb on + strand, within HMPREF1058_RS01650 at 76.365 kb on + strand, within HMPREF1058_RS01650 at 76.366 kb on - strand, within HMPREF1058_RS01650 at 76.378 kb on + strand, within HMPREF1058_RS01650 at 76.380 kb on + strand, within HMPREF1058_RS01650 at 76.381 kb on - strand, within HMPREF1058_RS01650 at 76.443 kb on - strand at 76.444 kb on + strand at 76.444 kb on + strand at 76.444 kb on + strand at 76.445 kb on - strand at 76.445 kb on - strand at 76.445 kb on - strand at 76.445 kb on - strand at 76.491 kb on + strand at 76.505 kb on + strand at 76.614 kb on + strand, within HMPREF1058_RS01645 at 76.617 kb on - strand, within HMPREF1058_RS01645 at 76.619 kb on - strand, within HMPREF1058_RS01645 at 76.630 kb on + strand, within HMPREF1058_RS01645 at 76.847 kb on + strand, within HMPREF1058_RS01645 at 76.848 kb on - strand, within HMPREF1058_RS01645 at 76.878 kb on + strand, within HMPREF1058_RS01645 at 76.879 kb on - strand, within HMPREF1058_RS01645 at 76.879 kb on - strand, within HMPREF1058_RS01645 at 76.939 kb on + strand, within HMPREF1058_RS01645 at 76.940 kb on - strand, within HMPREF1058_RS01645 at 77.005 kb on + strand, within HMPREF1058_RS01645 at 77.006 kb on - strand, within HMPREF1058_RS01645 at 77.282 kb on + strand at 77.315 kb on + strand at 77.329 kb on - strand at 77.329 kb on - strand at 77.340 kb on + strand at 77.392 kb on - strand at 77.392 kb on - strand at 77.393 kb on + strand at 77.393 kb on + strand at 77.394 kb on - strand at 77.394 kb on - strand at 77.394 kb on - strand at 77.394 kb on - strand at 77.395 kb on + strand at 77.396 kb on - strand at 77.396 kb on - strand at 77.396 kb on - strand at 77.396 kb on - strand at 77.405 kb on + strand at 77.406 kb on - strand at 77.408 kb on + strand at 77.417 kb on - strand at 77.417 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Mannose 10 mM (C) remove 75,519 + HMPREF1058_RS01655 0.40 +0.2 75,519 + HMPREF1058_RS01655 0.40 -2.8 75,520 - HMPREF1058_RS01655 0.40 +0.5 75,524 + HMPREF1058_RS01655 0.41 -1.7 75,638 + HMPREF1058_RS01655 0.52 -3.0 75,639 - HMPREF1058_RS01655 0.52 -0.8 75,640 + HMPREF1058_RS01655 0.53 -2.6 75,640 + HMPREF1058_RS01655 0.53 -3.3 75,641 - HMPREF1058_RS01655 0.53 -5.2 75,641 - HMPREF1058_RS01655 0.53 -2.7 75,643 - HMPREF1058_RS01655 0.53 -0.1 75,643 - HMPREF1058_RS01655 0.53 -2.3 75,756 - HMPREF1058_RS01655 0.64 -1.5 75,757 + HMPREF1058_RS01655 0.65 -4.0 75,760 - HMPREF1058_RS01655 0.65 -4.6 75,809 + HMPREF1058_RS01655 0.70 -3.0 75,809 + HMPREF1058_RS01655 0.70 -1.6 75,810 - HMPREF1058_RS01655 0.70 -2.5 75,811 + HMPREF1058_RS01655 0.70 -3.0 75,811 + HMPREF1058_RS01655 0.70 -2.2 75,893 - HMPREF1058_RS01655 0.78 -1.3 76,146 - -3.4 76,171 + HMPREF1058_RS01650 0.16 -0.1 76,175 + HMPREF1058_RS01650 0.18 -2.5 76,175 + HMPREF1058_RS01650 0.18 -3.4 76,176 - HMPREF1058_RS01650 0.18 -3.5 76,176 - HMPREF1058_RS01650 0.18 -2.4 76,365 + HMPREF1058_RS01650 0.76 -2.1 76,365 + HMPREF1058_RS01650 0.76 -3.7 76,366 - HMPREF1058_RS01650 0.77 -3.3 76,378 + HMPREF1058_RS01650 0.80 -2.6 76,380 + HMPREF1058_RS01650 0.81 -5.1 76,381 - HMPREF1058_RS01650 0.81 -3.2 76,443 - -0.4 76,444 + -0.8 76,444 + -0.7 76,444 + -1.5 76,445 - +2.5 76,445 - -1.6 76,445 - +0.5 76,445 - -0.5 76,491 + -1.0 76,505 + -2.8 76,614 + HMPREF1058_RS01645 0.13 -0.4 76,617 - HMPREF1058_RS01645 0.14 -1.1 76,619 - HMPREF1058_RS01645 0.14 -1.0 76,630 + HMPREF1058_RS01645 0.15 -0.7 76,847 + HMPREF1058_RS01645 0.44 -1.3 76,848 - HMPREF1058_RS01645 0.44 -1.9 76,878 + HMPREF1058_RS01645 0.48 -1.5 76,879 - HMPREF1058_RS01645 0.48 -0.1 76,879 - HMPREF1058_RS01645 0.48 -2.0 76,939 + HMPREF1058_RS01645 0.56 -0.5 76,940 - HMPREF1058_RS01645 0.56 +0.0 77,005 + HMPREF1058_RS01645 0.65 -1.1 77,006 - HMPREF1058_RS01645 0.65 -1.2 77,282 + +0.3 77,315 + -0.0 77,329 - -1.1 77,329 - -1.5 77,340 + +0.7 77,392 - -2.0 77,392 - -0.9 77,393 + -1.7 77,393 + -0.5 77,394 - -1.3 77,394 - -2.8 77,394 - -2.6 77,394 - -2.4 77,395 + -1.1 77,396 - -0.5 77,396 - -2.1 77,396 - -1.1 77,396 - -3.6 77,405 + -2.7 77,406 - -2.4 77,408 + -2.8 77,417 - -0.1 77,417 - +0.7
Or see this region's nucleotide sequence