Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00135

Experiment: McCas_formate_complete potassium hexahydroxoantimonate 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00120 and MMP_RS00125 are separated by 29 nucleotidesMMP_RS00125 and MMP_RS00130 are separated by 91 nucleotidesMMP_RS00130 and MMP_RS00135 are separated by 116 nucleotidesMMP_RS00135 and MMP_RS00140 are separated by 4 nucleotidesMMP_RS00140 and MMP_RS00145 overlap by 4 nucleotides MMP_RS00120: MMP_RS00120 - LysR family transcriptional regulator, at 27,798 to 28,640 _RS00120 MMP_RS00125: MMP_RS00125 - DUF2798 domain-containing protein, at 28,670 to 28,897 _RS00125 MMP_RS00130: MMP_RS00130 - nickel-responsive transcriptional regulator NikR, at 28,989 to 29,414 _RS00130 MMP_RS00135: MMP_RS00135 - DUF2121 domain-containing protein, at 29,531 to 30,406 _RS00135 MMP_RS00140: MMP_RS00140 - DUF192 domain-containing protein, at 30,411 to 30,746 _RS00140 MMP_RS00145: MMP_RS00145 - ATP-dependent Clp protease proteolytic subunit, at 30,743 to 31,567 _RS00145 Position (kb) 29 30 31Strain fitness (log2 ratio) -2 -1 0 1at 28.609 kb on - strandat 28.657 kb on + strandat 28.659 kb on - strandat 28.660 kb on + strandat 28.683 kb on + strandat 28.829 kb on - strand, within MMP_RS00125at 28.900 kb on + strandat 28.900 kb on + strandat 28.924 kb on - strandat 29.300 kb on - strand, within MMP_RS00130at 29.390 kb on - strandat 29.390 kb on - strandat 29.390 kb on - strandat 29.417 kb on - strandat 29.417 kb on - strandat 29.418 kb on + strandat 29.453 kb on + strandat 29.459 kb on - strandat 29.516 kb on + strandat 29.746 kb on + strand, within MMP_RS00135at 29.770 kb on + strand, within MMP_RS00135at 29.770 kb on + strand, within MMP_RS00135at 29.807 kb on - strand, within MMP_RS00135at 29.807 kb on - strand, within MMP_RS00135at 30.051 kb on + strand, within MMP_RS00135at 30.051 kb on + strand, within MMP_RS00135at 30.231 kb on - strand, within MMP_RS00135at 30.253 kb on - strand, within MMP_RS00135at 30.262 kb on + strand, within MMP_RS00135at 30.262 kb on + strand, within MMP_RS00135at 30.479 kb on - strand, within MMP_RS00140at 30.489 kb on - strand, within MMP_RS00140at 30.520 kb on + strand, within MMP_RS00140at 30.534 kb on + strand, within MMP_RS00140at 30.571 kb on + strand, within MMP_RS00140at 30.703 kb on + strand, within MMP_RS00140at 30.723 kb on - strandat 30.780 kb on - strandat 30.780 kb on - strandat 30.780 kb on - strandat 30.841 kb on - strand, within MMP_RS00145at 30.890 kb on + strand, within MMP_RS00145at 30.926 kb on + strand, within MMP_RS00145at 31.128 kb on + strand, within MMP_RS00145at 31.278 kb on + strand, within MMP_RS00145at 31.319 kb on + strand, within MMP_RS00145at 31.319 kb on + strand, within MMP_RS00145at 31.337 kb on + strand, within MMP_RS00145at 31.372 kb on - strand, within MMP_RS00145at 31.377 kb on - strand, within MMP_RS00145at 31.378 kb on + strand, within MMP_RS00145at 31.378 kb on + strand, within MMP_RS00145at 31.382 kb on + strand, within MMP_RS00145

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete potassium hexahydroxoantimonate 0.5 mM
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28,609 - +0.0
28,657 + -0.3
28,659 - +0.6
28,660 + -0.2
28,683 + +0.4
28,829 - MMP_RS00125 0.70 -0.8
28,900 + +0.5
28,900 + +0.4
28,924 - -0.1
29,300 - MMP_RS00130 0.73 +0.5
29,390 - +0.4
29,390 - -0.0
29,390 - +0.5
29,417 - +0.9
29,417 - -0.2
29,418 + -0.5
29,453 + -0.4
29,459 - +0.0
29,516 + +0.2
29,746 + MMP_RS00135 0.25 -0.0
29,770 + MMP_RS00135 0.27 -1.0
29,770 + MMP_RS00135 0.27 +0.2
29,807 - MMP_RS00135 0.32 -0.0
29,807 - MMP_RS00135 0.32 -0.2
30,051 + MMP_RS00135 0.59 -0.7
30,051 + MMP_RS00135 0.59 -1.2
30,231 - MMP_RS00135 0.80 -0.1
30,253 - MMP_RS00135 0.82 +0.3
30,262 + MMP_RS00135 0.83 +0.2
30,262 + MMP_RS00135 0.83 -0.0
30,479 - MMP_RS00140 0.20 +0.4
30,489 - MMP_RS00140 0.23 +0.3
30,520 + MMP_RS00140 0.32 -0.1
30,534 + MMP_RS00140 0.37 +0.3
30,571 + MMP_RS00140 0.48 -0.9
30,703 + MMP_RS00140 0.87 +0.4
30,723 - +0.4
30,780 - +0.3
30,780 - +0.7
30,780 - +0.5
30,841 - MMP_RS00145 0.12 -0.4
30,890 + MMP_RS00145 0.18 +0.5
30,926 + MMP_RS00145 0.22 +0.5
31,128 + MMP_RS00145 0.47 -1.9
31,278 + MMP_RS00145 0.65 -0.6
31,319 + MMP_RS00145 0.70 -0.4
31,319 + MMP_RS00145 0.70 -0.5
31,337 + MMP_RS00145 0.72 +1.0
31,372 - MMP_RS00145 0.76 +0.6
31,377 - MMP_RS00145 0.77 -0.2
31,378 + MMP_RS00145 0.77 -1.1
31,378 + MMP_RS00145 0.77 -0.7
31,382 + MMP_RS00145 0.77 -1.3

Or see this region's nucleotide sequence