Strain Fitness in Methanococcus maripaludis S2 around MMP_RS00505

Experiment: McCas_formate_complete 1,2-dimethyl-5-nitroimidazole 0.00416666666666667 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS00500 and MMP_RS00505 are separated by 4 nucleotidesMMP_RS00505 and MMP_RS00510 are separated by 14 nucleotides MMP_RS00500: MMP_RS00500 - glutamyl-tRNA reductase, at 98,977 to 100,125 _RS00500 MMP_RS00505: MMP_RS00505 - bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase, at 100,130 to 100,735 _RS00505 MMP_RS00510: MMP_RS00510 - glycosyltransferase family 4 protein, at 100,750 to 101,817 _RS00510 Position (kb) 100 101Strain fitness (log2 ratio) -3 -2 -1 0 1at 100.089 kb on - strandat 100.771 kb on + strandat 100.774 kb on - strandat 100.781 kb on - strandat 101.209 kb on + strandat 101.318 kb on - strandat 101.319 kb on + strandat 101.612 kb on - strandat 101.729 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction McCas_formate_complete 1,2-dimethyl-5-nitroimidazole 0.00416666666666667 mM
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100,089 - -2.8
100,771 + -0.9
100,774 - +0.3
100,781 - -3.1
101,209 + -2.1
101,318 - -1.1
101,319 + -3.5
101,612 - -3.5
101,729 + -2.8

Or see this region's nucleotide sequence