Experiment: McCas_formate_complete Magnesium chloride 250 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03195 and MMP_RS03200 are separated by 36 nucleotides MMP_RS03200 and MMP_RS03205 are separated by 136 nucleotides MMP_RS03205 and MMP_RS03210 are separated by 147 nucleotides
MMP_RS03195: MMP_RS03195 - global nitrogen regulator NrpR, at 601,455 to 603,065
_RS03195
MMP_RS03200: MMP_RS03200 - methanogenesis marker 15 protein, at 603,102 to 604,337
_RS03200
MMP_RS03205: MMP_RS03205 - peptidyl-tRNA hydrolase Pth2, at 604,474 to 604,824
_RS03205
MMP_RS03210: MMP_RS03210 - tRNA guanosine(15) transglycosylase TgtA, at 604,972 to 606,921
_RS03210
Position (kb)
603
604
605 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 602.332 kb on + strand at 602.616 kb on + strand at 602.640 kb on - strand at 602.646 kb on + strand at 603.390 kb on - strand at 603.854 kb on - strand at 604.216 kb on - strand at 604.242 kb on + strand at 604.429 kb on - strand at 604.430 kb on + strand at 604.434 kb on - strand at 604.475 kb on - strand at 604.481 kb on + strand at 604.486 kb on - strand at 604.545 kb on - strand, within MMP_RS03205 at 604.699 kb on + strand, within MMP_RS03205 at 604.821 kb on + strand at 604.876 kb on + strand at 604.879 kb on + strand at 604.880 kb on - strand at 604.881 kb on + strand at 604.881 kb on + strand at 604.885 kb on + strand at 604.885 kb on + strand at 604.885 kb on + strand at 604.885 kb on + strand at 604.916 kb on - strand at 604.961 kb on + strand at 604.961 kb on + strand at 605.147 kb on - strand at 605.270 kb on - strand, within MMP_RS03210 at 605.270 kb on - strand, within MMP_RS03210 at 605.271 kb on + strand, within MMP_RS03210
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_formate_complete Magnesium chloride 250 mM remove 602,332 + -1.1 602,616 + -2.7 602,640 - -1.5 602,646 + -2.4 603,390 - -3.3 603,854 - -0.2 604,216 - -2.6 604,242 + -0.8 604,429 - -0.3 604,430 + -0.2 604,434 - +1.2 604,475 - -1.0 604,481 + -1.2 604,486 - -1.2 604,545 - MMP_RS03205 0.20 -3.0 604,699 + MMP_RS03205 0.64 -3.7 604,821 + -2.9 604,876 + -0.2 604,879 + +0.4 604,880 - -1.5 604,881 + +0.7 604,881 + +0.7 604,885 + +0.9 604,885 + -0.2 604,885 + +0.0 604,885 + +1.9 604,916 - -0.9 604,961 + -0.9 604,961 + +0.0 605,147 - +0.5 605,270 - MMP_RS03210 0.15 -0.3 605,270 - MMP_RS03210 0.15 -0.5 605,271 + MMP_RS03210 0.15 +0.4
Or see this region's nucleotide sequence