Experiment: TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_04546 and MPMX20_04547 are separated by 112 nucleotides MPMX20_04547 and MPMX20_04548 are separated by 111 nucleotides MPMX20_04548 and MPMX20_04549 are separated by 33 nucleotides
MPMX20_04546: MPMX20_04546 - hypothetical protein, at 4,759,518 to 4,759,937
_04546
MPMX20_04547: MPMX20_04547 - hypothetical protein, at 4,760,050 to 4,760,646
_04547
MPMX20_04548: MPMX20_04548 - Triosephosphate isomerase, at 4,760,758 to 4,761,525
_04548
MPMX20_04549: MPMX20_04549 - hypothetical protein, at 4,761,559 to 4,762,863
_04549
Position (kb) 4760
4761
4762 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 4759.785 kb on - strand, within MPMX20_04546 at 4759.815 kb on - strand, within MPMX20_04546 at 4759.933 kb on - strand at 4759.935 kb on + strand at 4759.942 kb on + strand at 4759.987 kb on - strand at 4760.047 kb on - strand at 4760.047 kb on - strand at 4760.076 kb on + strand at 4760.131 kb on + strand, within MPMX20_04547 at 4760.143 kb on + strand, within MPMX20_04547 at 4760.227 kb on - strand, within MPMX20_04547 at 4760.228 kb on - strand, within MPMX20_04547 at 4760.270 kb on + strand, within MPMX20_04547 at 4760.275 kb on + strand, within MPMX20_04547 at 4760.278 kb on - strand, within MPMX20_04547 at 4760.278 kb on - strand, within MPMX20_04547 at 4760.283 kb on - strand, within MPMX20_04547 at 4760.283 kb on - strand, within MPMX20_04547 at 4760.283 kb on - strand, within MPMX20_04547 at 4760.334 kb on - strand, within MPMX20_04547 at 4760.369 kb on + strand, within MPMX20_04547 at 4760.387 kb on + strand, within MPMX20_04547 at 4760.395 kb on - strand, within MPMX20_04547 at 4760.395 kb on - strand, within MPMX20_04547 at 4760.395 kb on - strand, within MPMX20_04547 at 4760.427 kb on + strand, within MPMX20_04547 at 4760.440 kb on + strand, within MPMX20_04547 at 4760.466 kb on - strand, within MPMX20_04547 at 4760.545 kb on + strand, within MPMX20_04547 at 4760.545 kb on + strand, within MPMX20_04547 at 4760.545 kb on + strand, within MPMX20_04547 at 4760.553 kb on - strand, within MPMX20_04547 at 4760.712 kb on + strand at 4761.500 kb on + strand at 4761.630 kb on + strand at 4761.630 kb on + strand at 4761.630 kb on + strand at 4761.655 kb on - strand at 4761.700 kb on + strand, within MPMX20_04549 at 4761.813 kb on + strand, within MPMX20_04549 at 4761.813 kb on - strand, within MPMX20_04549 at 4761.815 kb on + strand, within MPMX20_04549 at 4761.880 kb on + strand, within MPMX20_04549 at 4762.098 kb on + strand, within MPMX20_04549 at 4762.291 kb on - strand, within MPMX20_04549 at 4762.402 kb on - strand, within MPMX20_04549 at 4762.422 kb on + strand, within MPMX20_04549 at 4762.474 kb on - strand, within MPMX20_04549
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2 remove 4,759,785 - MPMX20_04546 0.64 -2.7 4,759,815 - MPMX20_04546 0.71 +1.3 4,759,933 - -0.3 4,759,935 + +1.6 4,759,942 + +4.9 4,759,987 - +0.0 4,760,047 - +0.1 4,760,047 - -1.9 4,760,076 + -0.3 4,760,131 + MPMX20_04547 0.14 -0.1 4,760,143 + MPMX20_04547 0.16 +0.8 4,760,227 - MPMX20_04547 0.30 -0.5 4,760,228 - MPMX20_04547 0.30 +1.2 4,760,270 + MPMX20_04547 0.37 -0.9 4,760,275 + MPMX20_04547 0.38 -1.0 4,760,278 - MPMX20_04547 0.38 +0.7 4,760,278 - MPMX20_04547 0.38 -3.7 4,760,283 - MPMX20_04547 0.39 +0.3 4,760,283 - MPMX20_04547 0.39 +2.4 4,760,283 - MPMX20_04547 0.39 -1.0 4,760,334 - MPMX20_04547 0.48 +0.5 4,760,369 + MPMX20_04547 0.53 -0.8 4,760,387 + MPMX20_04547 0.56 +5.4 4,760,395 - MPMX20_04547 0.58 -0.9 4,760,395 - MPMX20_04547 0.58 -0.3 4,760,395 - MPMX20_04547 0.58 -1.2 4,760,427 + MPMX20_04547 0.63 -2.9 4,760,440 + MPMX20_04547 0.65 +1.1 4,760,466 - MPMX20_04547 0.70 -2.7 4,760,545 + MPMX20_04547 0.83 +0.3 4,760,545 + MPMX20_04547 0.83 +0.5 4,760,545 + MPMX20_04547 0.83 -1.4 4,760,553 - MPMX20_04547 0.84 -0.5 4,760,712 + -1.2 4,761,500 + +2.9 4,761,630 + -1.7 4,761,630 + +3.1 4,761,630 + -0.5 4,761,655 - -0.8 4,761,700 + MPMX20_04549 0.11 +3.1 4,761,813 + MPMX20_04549 0.19 +1.3 4,761,813 - MPMX20_04549 0.19 -0.7 4,761,815 + MPMX20_04549 0.20 -1.9 4,761,880 + MPMX20_04549 0.25 +2.9 4,762,098 + MPMX20_04549 0.41 -0.9 4,762,291 - MPMX20_04549 0.56 -0.8 4,762,402 - MPMX20_04549 0.65 -1.1 4,762,422 + MPMX20_04549 0.66 -0.0 4,762,474 - MPMX20_04549 0.70 +2.1
Or see this region's nucleotide sequence