Experiment: TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_02495 and MPMX20_02496 are separated by 6 nucleotides MPMX20_02496 and MPMX20_02497 are separated by 148 nucleotides MPMX20_02497 and MPMX20_02498 are separated by 197 nucleotides MPMX20_02498 and MPMX20_02499 are separated by 44 nucleotides
MPMX20_02495: MPMX20_02495 - Lipopolysaccharide assembly protein B, at 2,634,473 to 2,635,642
_02495
MPMX20_02496: MPMX20_02496 - Lipopolysaccharide assembly protein A, at 2,635,649 to 2,635,957
_02496
MPMX20_02497: MPMX20_02497 - Phosphatidylglycerophosphatase B, at 2,636,106 to 2,636,873
_02497
MPMX20_02498: MPMX20_02498 - GTP cyclohydrolase-2, at 2,637,071 to 2,637,661
_02498
MPMX20_02499: MPMX20_02499 - Aconitate hydratase A, at 2,637,706 to 2,640,381
_02499
Position (kb)
2636
2637 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2635.677 kb on - strand at 2635.695 kb on - strand, within MPMX20_02496 at 2635.784 kb on - strand, within MPMX20_02496 at 2635.984 kb on - strand at 2636.001 kb on - strand at 2636.001 kb on - strand at 2636.158 kb on + strand at 2636.213 kb on + strand, within MPMX20_02497 at 2636.215 kb on + strand, within MPMX20_02497 at 2636.301 kb on - strand, within MPMX20_02497 at 2636.301 kb on - strand, within MPMX20_02497 at 2636.321 kb on + strand, within MPMX20_02497 at 2636.345 kb on + strand, within MPMX20_02497 at 2636.353 kb on - strand, within MPMX20_02497 at 2636.363 kb on - strand, within MPMX20_02497 at 2636.387 kb on + strand, within MPMX20_02497 at 2636.470 kb on + strand, within MPMX20_02497 at 2636.601 kb on + strand, within MPMX20_02497 at 2636.702 kb on - strand, within MPMX20_02497 at 2636.726 kb on + strand, within MPMX20_02497 at 2636.738 kb on + strand, within MPMX20_02497 at 2636.746 kb on - strand, within MPMX20_02497 at 2636.747 kb on + strand, within MPMX20_02497 at 2636.796 kb on + strand, within MPMX20_02497 at 2636.805 kb on - strand at 2636.856 kb on - strand at 2636.981 kb on - strand at 2637.733 kb on + strand at 2637.853 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2 remove 2,635,677 - +1.9 2,635,695 - MPMX20_02496 0.15 -1.3 2,635,784 - MPMX20_02496 0.44 -0.3 2,635,984 - -2.2 2,636,001 - +0.9 2,636,001 - +0.6 2,636,158 + -0.1 2,636,213 + MPMX20_02497 0.14 -0.7 2,636,215 + MPMX20_02497 0.14 -1.1 2,636,301 - MPMX20_02497 0.25 -1.9 2,636,301 - MPMX20_02497 0.25 -1.4 2,636,321 + MPMX20_02497 0.28 -1.8 2,636,345 + MPMX20_02497 0.31 -1.9 2,636,353 - MPMX20_02497 0.32 -0.6 2,636,363 - MPMX20_02497 0.33 -0.1 2,636,387 + MPMX20_02497 0.37 -0.6 2,636,470 + MPMX20_02497 0.47 -1.6 2,636,601 + MPMX20_02497 0.64 -1.4 2,636,702 - MPMX20_02497 0.78 +1.7 2,636,726 + MPMX20_02497 0.81 +0.5 2,636,738 + MPMX20_02497 0.82 -1.5 2,636,746 - MPMX20_02497 0.83 +1.4 2,636,747 + MPMX20_02497 0.83 +1.3 2,636,796 + MPMX20_02497 0.90 -0.5 2,636,805 - +0.4 2,636,856 - -0.7 2,636,981 - -0.8 2,637,733 + +0.6 2,637,853 - -2.9
Or see this region's nucleotide sequence