Experiment: TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01011 and MPMX20_01012 are separated by 24 nucleotides MPMX20_01012 and MPMX20_01013 are separated by 0 nucleotides MPMX20_01013 and MPMX20_01014 are separated by 312 nucleotides
MPMX20_01011: MPMX20_01011 - 1-deoxy-D-xylulose-5-phosphate synthase, at 1,063,398 to 1,065,260
_01011
MPMX20_01012: MPMX20_01012 - Farnesyl diphosphate synthase, at 1,065,285 to 1,066,184
_01012
MPMX20_01013: MPMX20_01013 - Exodeoxyribonuclease 7 small subunit, at 1,066,185 to 1,066,427
_01013
MPMX20_01014: MPMX20_01014 - tRNA sulfurtransferase, at 1,066,740 to 1,068,188
_01014
Position (kb)
1065
1066
1067 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1066.448 kb on - strand at 1066.500 kb on + strand at 1066.500 kb on + strand at 1066.500 kb on + strand at 1066.508 kb on - strand at 1066.508 kb on - strand at 1066.508 kb on - strand at 1066.508 kb on - strand at 1066.508 kb on - strand at 1066.520 kb on + strand at 1066.520 kb on - strand at 1066.537 kb on + strand at 1066.553 kb on + strand at 1066.553 kb on + strand at 1066.553 kb on + strand at 1066.554 kb on - strand at 1066.561 kb on - strand at 1066.561 kb on - strand at 1066.561 kb on - strand at 1066.561 kb on - strand at 1066.561 kb on - strand at 1066.561 kb on - strand at 1066.561 kb on - strand at 1066.561 kb on - strand at 1066.561 kb on - strand at 1066.563 kb on + strand at 1066.563 kb on + strand at 1066.563 kb on + strand at 1066.563 kb on + strand at 1066.571 kb on + strand at 1066.571 kb on - strand at 1066.571 kb on - strand at 1066.571 kb on - strand at 1066.571 kb on - strand at 1066.585 kb on - strand at 1066.587 kb on + strand at 1066.606 kb on - strand at 1066.628 kb on + strand at 1066.634 kb on + strand at 1066.636 kb on - strand at 1066.664 kb on + strand at 1066.709 kb on + strand at 1066.709 kb on + strand at 1066.709 kb on + strand at 1066.709 kb on + strand at 1066.710 kb on + strand at 1066.710 kb on + strand at 1066.710 kb on + strand at 1066.710 kb on + strand at 1066.717 kb on - strand at 1066.717 kb on - strand at 1066.717 kb on - strand at 1066.717 kb on - strand at 1066.717 kb on - strand at 1066.717 kb on - strand at 1066.717 kb on - strand at 1066.781 kb on + strand at 1066.877 kb on - strand at 1066.885 kb on + strand, within MPMX20_01014 at 1066.885 kb on + strand, within MPMX20_01014 at 1066.885 kb on + strand, within MPMX20_01014 at 1066.885 kb on + strand, within MPMX20_01014 at 1066.885 kb on + strand, within MPMX20_01014 at 1066.885 kb on + strand, within MPMX20_01014 at 1066.885 kb on + strand, within MPMX20_01014 at 1066.885 kb on + strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.893 kb on - strand, within MPMX20_01014 at 1066.942 kb on + strand, within MPMX20_01014 at 1066.942 kb on + strand, within MPMX20_01014 at 1066.942 kb on + strand, within MPMX20_01014 at 1066.950 kb on - strand, within MPMX20_01014 at 1066.950 kb on - strand, within MPMX20_01014 at 1066.950 kb on - strand, within MPMX20_01014 at 1066.950 kb on - strand, within MPMX20_01014 at 1066.986 kb on + strand, within MPMX20_01014 at 1066.986 kb on + strand, within MPMX20_01014 at 1066.994 kb on - strand, within MPMX20_01014 at 1066.994 kb on - strand, within MPMX20_01014 at 1067.032 kb on + strand, within MPMX20_01014 at 1067.035 kb on - strand, within MPMX20_01014 at 1067.064 kb on - strand, within MPMX20_01014 at 1067.093 kb on + strand, within MPMX20_01014 at 1067.101 kb on + strand, within MPMX20_01014 at 1067.101 kb on - strand, within MPMX20_01014 at 1067.114 kb on + strand, within MPMX20_01014 at 1067.114 kb on + strand, within MPMX20_01014 at 1067.129 kb on - strand, within MPMX20_01014 at 1067.131 kb on + strand, within MPMX20_01014 at 1067.173 kb on + strand, within MPMX20_01014 at 1067.173 kb on + strand, within MPMX20_01014
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2 remove 1,066,448 - -3.2 1,066,500 + -3.9 1,066,500 + -0.7 1,066,500 + +1.0 1,066,508 - +1.6 1,066,508 - -1.0 1,066,508 - -2.2 1,066,508 - -2.2 1,066,508 - -0.0 1,066,520 + +2.5 1,066,520 - +2.5 1,066,537 + +3.0 1,066,553 + -1.4 1,066,553 + +0.3 1,066,553 + +1.1 1,066,554 - -0.3 1,066,561 - -0.2 1,066,561 - -2.7 1,066,561 - -1.4 1,066,561 - +0.7 1,066,561 - +0.6 1,066,561 - -1.5 1,066,561 - +1.5 1,066,561 - -1.5 1,066,561 - -0.9 1,066,563 + +0.7 1,066,563 + +1.1 1,066,563 + +0.1 1,066,563 + +0.1 1,066,571 + +0.7 1,066,571 - -1.3 1,066,571 - -1.7 1,066,571 - -1.2 1,066,571 - -0.3 1,066,585 - -0.5 1,066,587 + +0.2 1,066,606 - +0.7 1,066,628 + +2.0 1,066,634 + +1.2 1,066,636 - +1.1 1,066,664 + +0.8 1,066,709 + -2.6 1,066,709 + +0.2 1,066,709 + -0.8 1,066,709 + -1.0 1,066,710 + -2.7 1,066,710 + +0.7 1,066,710 + +1.8 1,066,710 + +1.8 1,066,717 - +2.0 1,066,717 - -1.9 1,066,717 - -0.5 1,066,717 - -0.5 1,066,717 - +0.2 1,066,717 - +1.4 1,066,717 - -0.5 1,066,781 + -0.7 1,066,877 - -2.2 1,066,885 + MPMX20_01014 0.10 -2.1 1,066,885 + MPMX20_01014 0.10 -0.2 1,066,885 + MPMX20_01014 0.10 +0.3 1,066,885 + MPMX20_01014 0.10 +0.4 1,066,885 + MPMX20_01014 0.10 +3.0 1,066,885 + MPMX20_01014 0.10 +0.8 1,066,885 + MPMX20_01014 0.10 -0.3 1,066,885 + MPMX20_01014 0.10 -1.8 1,066,893 - MPMX20_01014 0.11 -0.5 1,066,893 - MPMX20_01014 0.11 +0.4 1,066,893 - MPMX20_01014 0.11 -0.1 1,066,893 - MPMX20_01014 0.11 +1.6 1,066,893 - MPMX20_01014 0.11 -0.4 1,066,893 - MPMX20_01014 0.11 -1.9 1,066,893 - MPMX20_01014 0.11 -1.4 1,066,893 - MPMX20_01014 0.11 +0.1 1,066,893 - MPMX20_01014 0.11 -0.6 1,066,893 - MPMX20_01014 0.11 -1.6 1,066,942 + MPMX20_01014 0.14 -2.3 1,066,942 + MPMX20_01014 0.14 -0.8 1,066,942 + MPMX20_01014 0.14 -0.5 1,066,950 - MPMX20_01014 0.14 -1.3 1,066,950 - MPMX20_01014 0.14 -2.1 1,066,950 - MPMX20_01014 0.14 -1.6 1,066,950 - MPMX20_01014 0.14 +0.7 1,066,986 + MPMX20_01014 0.17 -1.7 1,066,986 + MPMX20_01014 0.17 -1.0 1,066,994 - MPMX20_01014 0.18 -1.2 1,066,994 - MPMX20_01014 0.18 -0.7 1,067,032 + MPMX20_01014 0.20 -1.7 1,067,035 - MPMX20_01014 0.20 -1.7 1,067,064 - MPMX20_01014 0.22 -1.0 1,067,093 + MPMX20_01014 0.24 +0.0 1,067,101 + MPMX20_01014 0.25 -3.0 1,067,101 - MPMX20_01014 0.25 +0.4 1,067,114 + MPMX20_01014 0.26 -2.6 1,067,114 + MPMX20_01014 0.26 +1.0 1,067,129 - MPMX20_01014 0.27 +0.2 1,067,131 + MPMX20_01014 0.27 +0.4 1,067,173 + MPMX20_01014 0.30 +1.8 1,067,173 + MPMX20_01014 0.30 -1.8
Or see this region's nucleotide sequence