Experiment: TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00729 and MPMX20_00730 are separated by 158 nucleotides MPMX20_00730 and MPMX20_00731 overlap by 8 nucleotides MPMX20_00731 and MPMX20_00732 are separated by 26 nucleotides
MPMX20_00729: MPMX20_00729 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 753,783 to 754,733
_00729
MPMX20_00730: MPMX20_00730 - Glucose-6-phosphate isomerase, at 754,892 to 755,446
_00730
MPMX20_00731: MPMX20_00731 - hypothetical protein, at 755,439 to 756,209
_00731
MPMX20_00732: MPMX20_00732 - PTS system mannose-specific EIID component, at 756,236 to 757,054
_00732
Position (kb) 754
755
756 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 754.750 kb on + strand at 754.751 kb on - strand at 754.799 kb on + strand at 754.799 kb on - strand at 754.867 kb on - strand at 754.878 kb on + strand at 754.881 kb on - strand at 754.896 kb on - strand at 754.924 kb on + strand at 754.924 kb on + strand at 754.925 kb on + strand at 754.930 kb on + strand at 754.935 kb on + strand at 754.935 kb on + strand at 754.935 kb on + strand at 755.128 kb on - strand, within MPMX20_00730 at 755.169 kb on + strand, within MPMX20_00730 at 755.169 kb on + strand, within MPMX20_00730 at 755.169 kb on + strand, within MPMX20_00730 at 755.177 kb on - strand, within MPMX20_00730 at 755.177 kb on - strand, within MPMX20_00730 at 755.200 kb on + strand, within MPMX20_00730 at 755.246 kb on + strand, within MPMX20_00730 at 755.246 kb on + strand, within MPMX20_00730 at 755.246 kb on + strand, within MPMX20_00730 at 755.332 kb on + strand, within MPMX20_00730 at 755.340 kb on - strand, within MPMX20_00730 at 755.342 kb on - strand, within MPMX20_00730 at 755.376 kb on - strand, within MPMX20_00730 at 755.446 kb on + strand at 755.464 kb on - strand at 755.572 kb on + strand, within MPMX20_00731 at 755.572 kb on + strand, within MPMX20_00731 at 755.606 kb on + strand, within MPMX20_00731 at 755.664 kb on + strand, within MPMX20_00731 at 755.664 kb on + strand, within MPMX20_00731 at 755.664 kb on + strand, within MPMX20_00731 at 755.665 kb on - strand, within MPMX20_00731 at 755.665 kb on - strand, within MPMX20_00731 at 755.750 kb on + strand, within MPMX20_00731 at 755.788 kb on - strand, within MPMX20_00731 at 755.804 kb on + strand, within MPMX20_00731 at 755.880 kb on + strand, within MPMX20_00731 at 755.880 kb on + strand, within MPMX20_00731 at 755.927 kb on - strand, within MPMX20_00731 at 755.931 kb on + strand, within MPMX20_00731 at 756.001 kb on - strand, within MPMX20_00731 at 756.006 kb on - strand, within MPMX20_00731 at 756.009 kb on + strand, within MPMX20_00731 at 756.063 kb on + strand, within MPMX20_00731 at 756.305 kb on - strand at 756.331 kb on + strand, within MPMX20_00732 at 756.349 kb on - strand, within MPMX20_00732 at 756.408 kb on - strand, within MPMX20_00732 at 756.441 kb on + strand, within MPMX20_00732
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2 remove 754,750 + -0.4 754,751 - +0.5 754,799 + -1.3 754,799 - -2.5 754,867 - -0.6 754,878 + -1.0 754,881 - -3.2 754,896 - +2.1 754,924 + +0.9 754,924 + +1.6 754,925 + +1.5 754,930 + +1.7 754,935 + -0.8 754,935 + -3.5 754,935 + -0.6 755,128 - MPMX20_00730 0.43 -3.4 755,169 + MPMX20_00730 0.50 -1.7 755,169 + MPMX20_00730 0.50 +1.0 755,169 + MPMX20_00730 0.50 -0.4 755,177 - MPMX20_00730 0.51 +0.1 755,177 - MPMX20_00730 0.51 -0.3 755,200 + MPMX20_00730 0.55 +1.9 755,246 + MPMX20_00730 0.64 +3.9 755,246 + MPMX20_00730 0.64 -0.6 755,246 + MPMX20_00730 0.64 -1.5 755,332 + MPMX20_00730 0.79 -1.3 755,340 - MPMX20_00730 0.81 -2.3 755,342 - MPMX20_00730 0.81 -2.6 755,376 - MPMX20_00730 0.87 +0.1 755,446 + +1.9 755,464 - -0.1 755,572 + MPMX20_00731 0.17 +0.7 755,572 + MPMX20_00731 0.17 -3.0 755,606 + MPMX20_00731 0.22 +1.4 755,664 + MPMX20_00731 0.29 +0.1 755,664 + MPMX20_00731 0.29 -0.9 755,664 + MPMX20_00731 0.29 +0.9 755,665 - MPMX20_00731 0.29 +0.2 755,665 - MPMX20_00731 0.29 +1.6 755,750 + MPMX20_00731 0.40 +1.0 755,788 - MPMX20_00731 0.45 -0.3 755,804 + MPMX20_00731 0.47 -2.2 755,880 + MPMX20_00731 0.57 -2.0 755,880 + MPMX20_00731 0.57 -2.2 755,927 - MPMX20_00731 0.63 +1.0 755,931 + MPMX20_00731 0.64 +0.7 756,001 - MPMX20_00731 0.73 -0.4 756,006 - MPMX20_00731 0.74 -0.2 756,009 + MPMX20_00731 0.74 -1.0 756,063 + MPMX20_00731 0.81 -0.4 756,305 - -2.1 756,331 + MPMX20_00732 0.12 +1.5 756,349 - MPMX20_00732 0.14 +0.6 756,408 - MPMX20_00732 0.21 -1.8 756,441 + MPMX20_00732 0.25 -1.9
Or see this region's nucleotide sequence