Experiment: TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00255 and MPMX20_00256 are separated by 12 nucleotides MPMX20_00256 and MPMX20_00257 are separated by 64 nucleotides MPMX20_00257 and MPMX20_00258 are separated by 154 nucleotides MPMX20_00258 and MPMX20_00259 are separated by 108 nucleotides
MPMX20_00255: MPMX20_00255 - Chorismate pyruvate-lyase, at 273,144 to 273,641
_00255
MPMX20_00256: MPMX20_00256 - 4-hydroxybenzoate octaprenyltransferase, at 273,654 to 274,523
_00256
MPMX20_00257: MPMX20_00257 - Glycerol-3-phosphate acyltransferase, at 274,588 to 277,008
_00257
MPMX20_00258: MPMX20_00258 - Diacylglycerol kinase, at 277,163 to 277,531
_00258
MPMX20_00259: MPMX20_00259 - LexA repressor, at 277,640 to 278,248
_00259
Position (kb) 274
275
276
277
278 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 273.630 kb on + strand at 273.630 kb on + strand at 274.502 kb on + strand at 274.516 kb on - strand at 274.577 kb on - strand at 274.626 kb on + strand at 274.626 kb on + strand at 274.626 kb on + strand at 274.634 kb on - strand at 274.634 kb on - strand at 274.639 kb on - strand at 274.885 kb on - strand, within MPMX20_00257 at 277.060 kb on - strand at 277.087 kb on + strand at 277.087 kb on - strand at 277.094 kb on + strand at 277.102 kb on - strand at 277.104 kb on + strand at 277.123 kb on - strand at 277.128 kb on - strand at 277.174 kb on - strand at 277.176 kb on + strand at 277.189 kb on - strand at 277.195 kb on + strand at 277.195 kb on + strand at 277.203 kb on - strand, within MPMX20_00258 at 277.203 kb on - strand, within MPMX20_00258 at 277.235 kb on + strand, within MPMX20_00258 at 277.235 kb on + strand, within MPMX20_00258 at 277.243 kb on - strand, within MPMX20_00258 at 277.344 kb on + strand, within MPMX20_00258 at 277.344 kb on + strand, within MPMX20_00258 at 277.344 kb on + strand, within MPMX20_00258 at 277.352 kb on - strand, within MPMX20_00258 at 277.352 kb on - strand, within MPMX20_00258 at 277.352 kb on - strand, within MPMX20_00258 at 277.354 kb on + strand, within MPMX20_00258 at 277.396 kb on - strand, within MPMX20_00258 at 277.438 kb on - strand, within MPMX20_00258 at 277.450 kb on - strand, within MPMX20_00258 at 277.456 kb on - strand, within MPMX20_00258 at 277.458 kb on - strand, within MPMX20_00258 at 277.458 kb on - strand, within MPMX20_00258 at 277.495 kb on + strand at 277.500 kb on + strand at 277.510 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Enterobacter_ludwigii_PH2 remove 273,630 + +1.0 273,630 + -0.1 274,502 + -0.3 274,516 - +0.9 274,577 - +1.0 274,626 + -1.9 274,626 + -0.4 274,626 + -1.9 274,634 - -0.8 274,634 - +2.9 274,639 - +0.9 274,885 - MPMX20_00257 0.12 -1.6 277,060 - -1.2 277,087 + -1.4 277,087 - -0.7 277,094 + +2.6 277,102 - +0.8 277,104 + +0.9 277,123 - +3.0 277,128 - +0.3 277,174 - -0.8 277,176 + +0.3 277,189 - -1.4 277,195 + -0.3 277,195 + +0.1 277,203 - MPMX20_00258 0.11 -0.5 277,203 - MPMX20_00258 0.11 -2.4 277,235 + MPMX20_00258 0.20 -1.7 277,235 + MPMX20_00258 0.20 -2.2 277,243 - MPMX20_00258 0.22 -1.4 277,344 + MPMX20_00258 0.49 +0.6 277,344 + MPMX20_00258 0.49 +0.1 277,344 + MPMX20_00258 0.49 +0.6 277,352 - MPMX20_00258 0.51 -1.9 277,352 - MPMX20_00258 0.51 -0.3 277,352 - MPMX20_00258 0.51 -1.0 277,354 + MPMX20_00258 0.52 -1.4 277,396 - MPMX20_00258 0.63 -1.0 277,438 - MPMX20_00258 0.75 -3.3 277,450 - MPMX20_00258 0.78 -0.3 277,456 - MPMX20_00258 0.79 +0.1 277,458 - MPMX20_00258 0.80 -1.3 277,458 - MPMX20_00258 0.80 +2.9 277,495 + -0.8 277,500 + -1.6 277,510 + -0.7
Or see this region's nucleotide sequence