Experiment: LB plate, 1:1 coculture with Enterobacter_ludwigii_PH2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00212 and MPMX20_00213 overlap by 4 nucleotides MPMX20_00213 and MPMX20_00214 are separated by 1 nucleotides MPMX20_00214 and MPMX20_00215 overlap by 17 nucleotides MPMX20_00215 and MPMX20_00216 overlap by 8 nucleotides
MPMX20_00212: MPMX20_00212 - 2-iminoacetate synthase, at 222,202 to 223,335
_00212
MPMX20_00213: MPMX20_00213 - Thiazole synthase, at 223,332 to 224,102
_00213
MPMX20_00214: MPMX20_00214 - Sulfur carrier protein ThiS, at 224,104 to 224,304
_00214
MPMX20_00215: MPMX20_00215 - Sulfur carrier protein ThiS adenylyltransferase, at 224,288 to 225,043
_00215
MPMX20_00216: MPMX20_00216 - Thiamine-phosphate synthase, at 225,036 to 225,671
_00216
Position (kb) 223
224
225 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 222.410 kb on - strand, within MPMX20_00212 at 222.525 kb on + strand, within MPMX20_00212 at 222.546 kb on + strand, within MPMX20_00212 at 222.599 kb on + strand, within MPMX20_00212 at 222.629 kb on - strand, within MPMX20_00212 at 222.696 kb on + strand, within MPMX20_00212 at 222.831 kb on + strand, within MPMX20_00212 at 222.839 kb on - strand, within MPMX20_00212 at 222.853 kb on + strand, within MPMX20_00212 at 222.989 kb on + strand, within MPMX20_00212 at 223.011 kb on + strand, within MPMX20_00212 at 223.015 kb on - strand, within MPMX20_00212 at 223.072 kb on + strand, within MPMX20_00212 at 223.080 kb on - strand, within MPMX20_00212 at 223.086 kb on + strand, within MPMX20_00212 at 223.086 kb on + strand, within MPMX20_00212 at 223.086 kb on + strand, within MPMX20_00212 at 223.193 kb on + strand, within MPMX20_00212 at 223.193 kb on + strand, within MPMX20_00212 at 223.196 kb on - strand, within MPMX20_00212 at 223.201 kb on - strand, within MPMX20_00212 at 223.206 kb on - strand, within MPMX20_00212 at 223.284 kb on + strand at 223.284 kb on + strand at 223.284 kb on + strand at 223.292 kb on - strand at 223.292 kb on - strand at 223.292 kb on - strand at 223.292 kb on - strand at 223.368 kb on + strand at 223.594 kb on - strand, within MPMX20_00213 at 223.648 kb on - strand, within MPMX20_00213 at 223.722 kb on - strand, within MPMX20_00213 at 223.734 kb on + strand, within MPMX20_00213 at 223.734 kb on + strand, within MPMX20_00213 at 223.794 kb on + strand, within MPMX20_00213 at 224.025 kb on - strand, within MPMX20_00213 at 224.043 kb on + strand at 224.100 kb on + strand at 224.133 kb on + strand, within MPMX20_00214 at 224.163 kb on - strand, within MPMX20_00214 at 224.164 kb on - strand, within MPMX20_00214 at 224.173 kb on - strand, within MPMX20_00214 at 224.255 kb on - strand, within MPMX20_00214 at 224.261 kb on - strand, within MPMX20_00214 at 224.278 kb on - strand, within MPMX20_00214 at 224.285 kb on + strand at 224.293 kb on - strand at 224.339 kb on + strand at 224.347 kb on - strand at 224.407 kb on + strand, within MPMX20_00215 at 224.407 kb on + strand, within MPMX20_00215 at 224.420 kb on - strand, within MPMX20_00215 at 224.446 kb on + strand, within MPMX20_00215 at 224.456 kb on - strand, within MPMX20_00215 at 224.495 kb on + strand, within MPMX20_00215 at 224.509 kb on - strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.672 kb on + strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.680 kb on - strand, within MPMX20_00215 at 224.690 kb on - strand, within MPMX20_00215 at 224.691 kb on - strand, within MPMX20_00215 at 224.695 kb on - strand, within MPMX20_00215 at 224.718 kb on + strand, within MPMX20_00215 at 224.875 kb on + strand, within MPMX20_00215 at 224.883 kb on - strand, within MPMX20_00215 at 224.885 kb on + strand, within MPMX20_00215 at 224.890 kb on + strand, within MPMX20_00215 at 224.890 kb on + strand, within MPMX20_00215 at 224.898 kb on - strand, within MPMX20_00215 at 224.901 kb on + strand, within MPMX20_00215 at 225.082 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB plate, 1:1 coculture with Enterobacter_ludwigii_PH2 remove 222,410 - MPMX20_00212 0.18 +0.1 222,525 + MPMX20_00212 0.28 +0.1 222,546 + MPMX20_00212 0.30 +0.1 222,599 + MPMX20_00212 0.35 +0.5 222,629 - MPMX20_00212 0.38 +1.1 222,696 + MPMX20_00212 0.44 +0.7 222,831 + MPMX20_00212 0.55 -1.3 222,839 - MPMX20_00212 0.56 -1.8 222,853 + MPMX20_00212 0.57 +0.9 222,989 + MPMX20_00212 0.69 +1.5 223,011 + MPMX20_00212 0.71 +1.1 223,015 - MPMX20_00212 0.72 -2.6 223,072 + MPMX20_00212 0.77 -2.6 223,080 - MPMX20_00212 0.77 -0.1 223,086 + MPMX20_00212 0.78 +0.7 223,086 + MPMX20_00212 0.78 -0.9 223,086 + MPMX20_00212 0.78 -0.2 223,193 + MPMX20_00212 0.87 +1.4 223,193 + MPMX20_00212 0.87 -3.5 223,196 - MPMX20_00212 0.88 +1.3 223,201 - MPMX20_00212 0.88 +0.8 223,206 - MPMX20_00212 0.89 +0.1 223,284 + +0.6 223,284 + +1.7 223,284 + +0.5 223,292 - -0.1 223,292 - +2.7 223,292 - +0.5 223,292 - +0.8 223,368 + -3.3 223,594 - MPMX20_00213 0.34 -0.4 223,648 - MPMX20_00213 0.41 -0.5 223,722 - MPMX20_00213 0.51 -1.8 223,734 + MPMX20_00213 0.52 +0.4 223,734 + MPMX20_00213 0.52 +1.1 223,794 + MPMX20_00213 0.60 -2.9 224,025 - MPMX20_00213 0.90 -0.1 224,043 + +3.0 224,100 + +2.0 224,133 + MPMX20_00214 0.14 -1.2 224,163 - MPMX20_00214 0.29 -1.9 224,164 - MPMX20_00214 0.30 +0.8 224,173 - MPMX20_00214 0.34 +1.4 224,255 - MPMX20_00214 0.75 -0.7 224,261 - MPMX20_00214 0.78 +2.3 224,278 - MPMX20_00214 0.87 +0.3 224,285 + -0.3 224,293 - +0.5 224,339 + -1.6 224,347 - +0.5 224,407 + MPMX20_00215 0.16 +1.7 224,407 + MPMX20_00215 0.16 -2.1 224,420 - MPMX20_00215 0.17 -2.5 224,446 + MPMX20_00215 0.21 +0.1 224,456 - MPMX20_00215 0.22 +2.7 224,495 + MPMX20_00215 0.27 +2.4 224,509 - MPMX20_00215 0.29 +2.7 224,672 + MPMX20_00215 0.51 -1.5 224,672 + MPMX20_00215 0.51 +1.7 224,672 + MPMX20_00215 0.51 -0.8 224,672 + MPMX20_00215 0.51 +0.5 224,672 + MPMX20_00215 0.51 +1.8 224,672 + MPMX20_00215 0.51 -0.0 224,672 + MPMX20_00215 0.51 -1.3 224,672 + MPMX20_00215 0.51 +0.2 224,680 - MPMX20_00215 0.52 +1.9 224,680 - MPMX20_00215 0.52 +1.0 224,680 - MPMX20_00215 0.52 -3.7 224,680 - MPMX20_00215 0.52 +0.4 224,680 - MPMX20_00215 0.52 +1.7 224,690 - MPMX20_00215 0.53 +0.6 224,691 - MPMX20_00215 0.53 +0.9 224,695 - MPMX20_00215 0.54 +2.0 224,718 + MPMX20_00215 0.57 +1.7 224,875 + MPMX20_00215 0.78 -2.8 224,883 - MPMX20_00215 0.79 +2.9 224,885 + MPMX20_00215 0.79 -2.6 224,890 + MPMX20_00215 0.80 -0.5 224,890 + MPMX20_00215 0.80 -0.1 224,898 - MPMX20_00215 0.81 +0.1 224,901 + MPMX20_00215 0.81 -1.7 225,082 + -0.2
Or see this region's nucleotide sequence