Experiment: LB plate, 1:1 coculture with Pseudomonas_plecoglossicida_TBS_049
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_01014 and MPMX20_01015 are separated by 45 nucleotides MPMX20_01015 and MPMX20_01016 overlap by 38 nucleotides MPMX20_01016 and MPMX20_01017 are separated by 203 nucleotides MPMX20_01017 and MPMX20_01018 are separated by 32 nucleotides
MPMX20_01014: MPMX20_01014 - tRNA sulfurtransferase, at 1,066,740 to 1,068,188
_01014
MPMX20_01015: MPMX20_01015 - Protein/nucleic acid deglycase 3, at 1,068,234 to 1,068,827
_01015
MPMX20_01016: MPMX20_01016 - 2-dehydropantoate 2-reductase, at 1,068,790 to 1,069,701
_01016
MPMX20_01017: MPMX20_01017 - hypothetical protein, at 1,069,905 to 1,070,396
_01017
MPMX20_01018: MPMX20_01018 - Inner membrane transport protein YajR, at 1,070,429 to 1,071,790
_01018
Position (kb)
1068
1069
1070 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1068.073 kb on + strand at 1068.081 kb on - strand at 1068.106 kb on - strand at 1068.117 kb on + strand at 1068.117 kb on + strand at 1068.117 kb on + strand at 1068.150 kb on + strand at 1068.181 kb on - strand at 1068.239 kb on - strand at 1068.288 kb on + strand at 1068.454 kb on + strand, within MPMX20_01015 at 1068.533 kb on - strand, within MPMX20_01015 at 1068.614 kb on + strand, within MPMX20_01015 at 1068.677 kb on - strand, within MPMX20_01015 at 1068.783 kb on - strand at 1068.810 kb on - strand at 1068.820 kb on - strand at 1068.833 kb on - strand at 1068.837 kb on + strand at 1068.837 kb on + strand at 1068.850 kb on - strand at 1068.922 kb on + strand, within MPMX20_01016 at 1069.018 kb on + strand, within MPMX20_01016 at 1069.074 kb on - strand, within MPMX20_01016 at 1069.207 kb on - strand, within MPMX20_01016 at 1069.207 kb on - strand, within MPMX20_01016 at 1069.379 kb on + strand, within MPMX20_01016 at 1069.379 kb on + strand, within MPMX20_01016 at 1069.422 kb on + strand, within MPMX20_01016 at 1069.422 kb on + strand, within MPMX20_01016 at 1069.472 kb on + strand, within MPMX20_01016 at 1069.484 kb on + strand, within MPMX20_01016 at 1069.560 kb on - strand, within MPMX20_01016 at 1069.589 kb on - strand, within MPMX20_01016 at 1069.597 kb on + strand, within MPMX20_01016 at 1069.605 kb on - strand, within MPMX20_01016 at 1069.605 kb on - strand, within MPMX20_01016 at 1069.618 kb on + strand at 1069.618 kb on + strand at 1069.618 kb on + strand at 1069.618 kb on + strand at 1069.618 kb on + strand at 1069.626 kb on + strand at 1069.626 kb on - strand at 1069.626 kb on - strand at 1069.626 kb on - strand at 1069.655 kb on + strand at 1069.663 kb on - strand at 1069.663 kb on - strand at 1069.673 kb on - strand at 1069.673 kb on - strand at 1069.679 kb on + strand at 1069.687 kb on - strand at 1069.744 kb on + strand at 1069.797 kb on + strand at 1069.801 kb on + strand at 1069.816 kb on + strand at 1069.854 kb on + strand at 1069.866 kb on + strand at 1069.874 kb on - strand at 1070.017 kb on + strand, within MPMX20_01017 at 1070.087 kb on + strand, within MPMX20_01017 at 1070.111 kb on + strand, within MPMX20_01017 at 1070.130 kb on - strand, within MPMX20_01017 at 1070.155 kb on + strand, within MPMX20_01017 at 1070.213 kb on - strand, within MPMX20_01017 at 1070.213 kb on - strand, within MPMX20_01017 at 1070.213 kb on - strand, within MPMX20_01017 at 1070.213 kb on - strand, within MPMX20_01017 at 1070.258 kb on + strand, within MPMX20_01017 at 1070.503 kb on + strand at 1070.511 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB plate, 1:1 coculture with Pseudomonas_plecoglossicida_TBS_049 remove 1,068,073 + +3.0 1,068,081 - -1.4 1,068,106 - -0.7 1,068,117 + -0.2 1,068,117 + -1.4 1,068,117 + -0.6 1,068,150 + +0.6 1,068,181 - -3.1 1,068,239 - -1.1 1,068,288 + +0.4 1,068,454 + MPMX20_01015 0.37 -0.7 1,068,533 - MPMX20_01015 0.50 -0.8 1,068,614 + MPMX20_01015 0.64 +0.4 1,068,677 - MPMX20_01015 0.75 +0.4 1,068,783 - +0.2 1,068,810 - -2.8 1,068,820 - -2.4 1,068,833 - -0.8 1,068,837 + +0.1 1,068,837 + -1.6 1,068,850 - -0.2 1,068,922 + MPMX20_01016 0.14 +0.7 1,069,018 + MPMX20_01016 0.25 +0.5 1,069,074 - MPMX20_01016 0.31 -2.3 1,069,207 - MPMX20_01016 0.46 -1.3 1,069,207 - MPMX20_01016 0.46 -0.0 1,069,379 + MPMX20_01016 0.65 +1.7 1,069,379 + MPMX20_01016 0.65 +0.3 1,069,422 + MPMX20_01016 0.69 -1.5 1,069,422 + MPMX20_01016 0.69 +1.5 1,069,472 + MPMX20_01016 0.75 +2.5 1,069,484 + MPMX20_01016 0.76 +2.8 1,069,560 - MPMX20_01016 0.84 -1.6 1,069,589 - MPMX20_01016 0.88 +1.0 1,069,597 + MPMX20_01016 0.88 -0.4 1,069,605 - MPMX20_01016 0.89 +0.9 1,069,605 - MPMX20_01016 0.89 -2.9 1,069,618 + -0.1 1,069,618 + +0.4 1,069,618 + +0.3 1,069,618 + -1.5 1,069,618 + -1.3 1,069,626 + +1.0 1,069,626 - -1.2 1,069,626 - +0.6 1,069,626 - +1.2 1,069,655 + -0.5 1,069,663 - -0.7 1,069,663 - +3.0 1,069,673 - +0.4 1,069,673 - +0.1 1,069,679 + -2.2 1,069,687 - +3.0 1,069,744 + -2.1 1,069,797 + +2.3 1,069,801 + +2.2 1,069,816 + +1.9 1,069,854 + +0.3 1,069,866 + -1.5 1,069,874 - -1.1 1,070,017 + MPMX20_01017 0.23 +1.4 1,070,087 + MPMX20_01017 0.37 -1.7 1,070,111 + MPMX20_01017 0.42 -3.4 1,070,130 - MPMX20_01017 0.46 -2.5 1,070,155 + MPMX20_01017 0.51 -1.8 1,070,213 - MPMX20_01017 0.63 -2.5 1,070,213 - MPMX20_01017 0.63 -1.8 1,070,213 - MPMX20_01017 0.63 +2.5 1,070,213 - MPMX20_01017 0.63 -0.1 1,070,258 + MPMX20_01017 0.72 -0.7 1,070,503 + +3.0 1,070,511 - -2.7
Or see this region's nucleotide sequence