Experiment: TSB plate, 1:1 coculture with Rhodococcus_aetherivorans_MH2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00744 and MPMX20_00745 are separated by 39 nucleotides MPMX20_00745 and MPMX20_00746 are separated by 4 nucleotides MPMX20_00746 and MPMX20_00747 are separated by 3 nucleotides MPMX20_00747 and MPMX20_00748 overlap by 4 nucleotides
MPMX20_00744: MPMX20_00744 - IS1595 family transposase ISSsu9, at 770,517 to 770,996
_00744
MPMX20_00745: MPMX20_00745 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical, at 771,036 to 771,884
_00745
MPMX20_00746: MPMX20_00746 - Protein ApaG, at 771,889 to 772,266
_00746
MPMX20_00747: MPMX20_00747 - Ribosomal RNA small subunit methyltransferase A, at 772,270 to 773,091
_00747
MPMX20_00748: MPMX20_00748 - 4-hydroxythreonine-4-phosphate dehydrogenase, at 773,088 to 774,074
_00748
Position (kb) 771
772
773 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7
8 at 771.024 kb on + strand at 771.123 kb on + strand, within MPMX20_00745 at 771.123 kb on + strand, within MPMX20_00745 at 771.123 kb on + strand, within MPMX20_00745 at 771.131 kb on - strand, within MPMX20_00745 at 771.131 kb on - strand, within MPMX20_00745 at 771.178 kb on - strand, within MPMX20_00745 at 771.234 kb on - strand, within MPMX20_00745 at 771.372 kb on + strand, within MPMX20_00745 at 771.380 kb on - strand, within MPMX20_00745 at 771.471 kb on - strand, within MPMX20_00745 at 771.596 kb on - strand, within MPMX20_00745 at 771.622 kb on + strand, within MPMX20_00745 at 771.693 kb on + strand, within MPMX20_00745 at 771.701 kb on - strand, within MPMX20_00745 at 771.701 kb on - strand, within MPMX20_00745 at 771.720 kb on - strand, within MPMX20_00745 at 771.814 kb on - strand at 771.821 kb on + strand at 771.842 kb on - strand at 771.870 kb on - strand at 771.976 kb on + strand, within MPMX20_00746 at 771.994 kb on - strand, within MPMX20_00746 at 771.994 kb on - strand, within MPMX20_00746 at 771.994 kb on - strand, within MPMX20_00746 at 772.046 kb on + strand, within MPMX20_00746 at 772.132 kb on + strand, within MPMX20_00746 at 772.268 kb on + strand at 772.276 kb on - strand at 772.321 kb on + strand at 772.329 kb on - strand at 772.329 kb on - strand at 772.442 kb on - strand, within MPMX20_00747 at 772.442 kb on - strand, within MPMX20_00747 at 772.459 kb on - strand, within MPMX20_00747 at 772.473 kb on + strand, within MPMX20_00747 at 772.506 kb on + strand, within MPMX20_00747 at 772.506 kb on + strand, within MPMX20_00747 at 772.526 kb on - strand, within MPMX20_00747 at 772.528 kb on + strand, within MPMX20_00747 at 772.553 kb on - strand, within MPMX20_00747 at 772.573 kb on - strand, within MPMX20_00747 at 772.626 kb on - strand, within MPMX20_00747 at 772.626 kb on - strand, within MPMX20_00747 at 772.724 kb on - strand, within MPMX20_00747 at 772.877 kb on - strand, within MPMX20_00747 at 772.920 kb on - strand, within MPMX20_00747 at 772.939 kb on - strand, within MPMX20_00747 at 772.944 kb on - strand, within MPMX20_00747 at 772.953 kb on - strand, within MPMX20_00747 at 772.980 kb on - strand, within MPMX20_00747 at 773.071 kb on - strand at 773.076 kb on - strand at 773.152 kb on + strand at 773.155 kb on - strand at 773.157 kb on + strand at 773.157 kb on + strand at 773.157 kb on + strand at 773.157 kb on + strand at 773.177 kb on + strand at 773.180 kb on - strand at 773.182 kb on + strand at 773.249 kb on - strand, within MPMX20_00748 at 773.249 kb on - strand, within MPMX20_00748 at 773.255 kb on - strand, within MPMX20_00748
Per-strain Table
Position Strand Gene LocusTag Fraction TSB plate, 1:1 coculture with Rhodococcus_aetherivorans_MH2 remove 771,024 + -3.0 771,123 + MPMX20_00745 0.10 +1.7 771,123 + MPMX20_00745 0.10 +1.2 771,123 + MPMX20_00745 0.10 -2.1 771,131 - MPMX20_00745 0.11 -1.6 771,131 - MPMX20_00745 0.11 -0.8 771,178 - MPMX20_00745 0.17 -0.7 771,234 - MPMX20_00745 0.23 +1.3 771,372 + MPMX20_00745 0.40 +1.5 771,380 - MPMX20_00745 0.41 -0.3 771,471 - MPMX20_00745 0.51 -0.6 771,596 - MPMX20_00745 0.66 +1.4 771,622 + MPMX20_00745 0.69 -0.7 771,693 + MPMX20_00745 0.77 +2.4 771,701 - MPMX20_00745 0.78 +0.5 771,701 - MPMX20_00745 0.78 +0.3 771,720 - MPMX20_00745 0.81 -0.6 771,814 - +0.6 771,821 + +1.9 771,842 - +0.2 771,870 - -0.6 771,976 + MPMX20_00746 0.23 +8.1 771,994 - MPMX20_00746 0.28 +0.8 771,994 - MPMX20_00746 0.28 -1.3 771,994 - MPMX20_00746 0.28 +0.5 772,046 + MPMX20_00746 0.42 -1.2 772,132 + MPMX20_00746 0.64 -1.2 772,268 + -1.6 772,276 - +0.1 772,321 + -0.6 772,329 - -2.2 772,329 - +2.9 772,442 - MPMX20_00747 0.21 -0.7 772,442 - MPMX20_00747 0.21 -0.5 772,459 - MPMX20_00747 0.23 +0.1 772,473 + MPMX20_00747 0.25 +0.9 772,506 + MPMX20_00747 0.29 -0.1 772,506 + MPMX20_00747 0.29 +1.9 772,526 - MPMX20_00747 0.31 +1.3 772,528 + MPMX20_00747 0.31 +0.5 772,553 - MPMX20_00747 0.34 -1.1 772,573 - MPMX20_00747 0.37 -0.3 772,626 - MPMX20_00747 0.43 -0.6 772,626 - MPMX20_00747 0.43 -3.5 772,724 - MPMX20_00747 0.55 -0.1 772,877 - MPMX20_00747 0.74 -0.8 772,920 - MPMX20_00747 0.79 -0.6 772,939 - MPMX20_00747 0.81 -2.1 772,944 - MPMX20_00747 0.82 -0.3 772,953 - MPMX20_00747 0.83 +1.9 772,980 - MPMX20_00747 0.86 +1.3 773,071 - -1.6 773,076 - -0.1 773,152 + -0.5 773,155 - -2.8 773,157 + -0.8 773,157 + -0.6 773,157 + -0.7 773,157 + +1.9 773,177 + -2.8 773,180 - +1.3 773,182 + +0.5 773,249 - MPMX20_00748 0.16 +0.5 773,249 - MPMX20_00748 0.16 -1.1 773,255 - MPMX20_00748 0.17 +0.1
Or see this region's nucleotide sequence