Experiment: RCH2_defined_glucose plate, 1:1 coculture with Enterobacter_TBS_079
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MPMX20_00522 and MPMX20_00523 are separated by 13 nucleotides MPMX20_00523 and MPMX20_00524 are separated by 42 nucleotides MPMX20_00524 and MPMX20_00525 overlap by 17 nucleotides
MPMX20_00522: MPMX20_00522 - hypothetical protein, at 538,237 to 539,013
_00522
MPMX20_00523: MPMX20_00523 - hypothetical protein, at 539,027 to 539,668
_00523
MPMX20_00524: MPMX20_00524 - Deacetylase, at 539,711 to 540,844
_00524
MPMX20_00525: MPMX20_00525 - D-glucosaminate-6-phosphate ammonia lyase, at 540,828 to 541,946
_00525
Position (kb) 539
540
541 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 538.775 kb on + strand, within MPMX20_00522 at 538.778 kb on + strand, within MPMX20_00522 at 538.794 kb on + strand, within MPMX20_00522 at 538.841 kb on + strand, within MPMX20_00522 at 538.855 kb on + strand, within MPMX20_00522 at 538.910 kb on - strand, within MPMX20_00522 at 538.982 kb on + strand at 539.043 kb on - strand at 539.043 kb on - strand at 539.076 kb on + strand at 539.086 kb on + strand at 539.089 kb on - strand at 539.094 kb on - strand, within MPMX20_00523 at 539.097 kb on - strand, within MPMX20_00523 at 539.141 kb on - strand, within MPMX20_00523 at 539.143 kb on + strand, within MPMX20_00523 at 539.260 kb on + strand, within MPMX20_00523 at 539.296 kb on - strand, within MPMX20_00523 at 539.296 kb on - strand, within MPMX20_00523 at 539.449 kb on - strand, within MPMX20_00523 at 539.481 kb on - strand, within MPMX20_00523 at 539.570 kb on - strand, within MPMX20_00523 at 539.574 kb on - strand, within MPMX20_00523 at 539.643 kb on - strand at 539.644 kb on - strand at 539.650 kb on + strand at 539.654 kb on - strand at 539.694 kb on - strand at 539.694 kb on - strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.723 kb on + strand at 539.731 kb on - strand at 539.731 kb on - strand at 539.756 kb on - strand at 539.761 kb on - strand at 539.841 kb on - strand, within MPMX20_00524 at 539.848 kb on - strand, within MPMX20_00524 at 539.854 kb on - strand, within MPMX20_00524 at 539.873 kb on - strand, within MPMX20_00524 at 539.873 kb on - strand, within MPMX20_00524 at 539.879 kb on + strand, within MPMX20_00524 at 539.923 kb on - strand, within MPMX20_00524 at 540.017 kb on + strand, within MPMX20_00524 at 540.035 kb on + strand, within MPMX20_00524 at 540.037 kb on - strand, within MPMX20_00524 at 540.048 kb on + strand, within MPMX20_00524 at 540.068 kb on + strand, within MPMX20_00524 at 540.076 kb on - strand, within MPMX20_00524 at 540.124 kb on - strand, within MPMX20_00524 at 540.195 kb on + strand, within MPMX20_00524 at 540.307 kb on - strand, within MPMX20_00524 at 540.309 kb on + strand, within MPMX20_00524 at 540.309 kb on + strand, within MPMX20_00524 at 540.309 kb on + strand, within MPMX20_00524 at 540.309 kb on + strand, within MPMX20_00524 at 540.314 kb on + strand, within MPMX20_00524 at 540.314 kb on + strand, within MPMX20_00524 at 540.317 kb on - strand, within MPMX20_00524 at 540.317 kb on - strand, within MPMX20_00524 at 540.317 kb on - strand, within MPMX20_00524 at 540.386 kb on + strand, within MPMX20_00524 at 540.479 kb on - strand, within MPMX20_00524 at 540.512 kb on - strand, within MPMX20_00524 at 540.678 kb on + strand, within MPMX20_00524 at 540.714 kb on - strand, within MPMX20_00524 at 540.791 kb on + strand at 540.797 kb on + strand at 540.817 kb on - strand at 540.817 kb on - strand at 540.824 kb on + strand at 540.827 kb on - strand at 540.827 kb on - strand at 540.853 kb on + strand at 540.873 kb on + strand at 540.874 kb on + strand at 540.975 kb on - strand, within MPMX20_00525 at 541.021 kb on + strand, within MPMX20_00525 at 541.029 kb on - strand, within MPMX20_00525 at 541.037 kb on - strand, within MPMX20_00525 at 541.037 kb on - strand, within MPMX20_00525 at 541.080 kb on + strand, within MPMX20_00525 at 541.244 kb on - strand, within MPMX20_00525 at 541.303 kb on + strand, within MPMX20_00525 at 541.305 kb on + strand, within MPMX20_00525 at 541.311 kb on - strand, within MPMX20_00525 at 541.311 kb on - strand, within MPMX20_00525 at 541.313 kb on - strand, within MPMX20_00525 at 541.334 kb on - strand, within MPMX20_00525 at 541.336 kb on + strand, within MPMX20_00525 at 541.336 kb on + strand, within MPMX20_00525 at 541.336 kb on + strand, within MPMX20_00525 at 541.344 kb on - strand, within MPMX20_00525 at 541.344 kb on - strand, within MPMX20_00525 at 541.344 kb on - strand, within MPMX20_00525 at 541.375 kb on - strand, within MPMX20_00525 at 541.429 kb on - strand, within MPMX20_00525 at 541.435 kb on - strand, within MPMX20_00525 at 541.586 kb on + strand, within MPMX20_00525 at 541.586 kb on + strand, within MPMX20_00525 at 541.594 kb on - strand, within MPMX20_00525 at 541.607 kb on + strand, within MPMX20_00525 at 541.630 kb on + strand, within MPMX20_00525 at 541.649 kb on + strand, within MPMX20_00525 at 541.671 kb on + strand, within MPMX20_00525 at 541.686 kb on - strand, within MPMX20_00525 at 541.704 kb on + strand, within MPMX20_00525 at 541.704 kb on + strand, within MPMX20_00525 at 541.704 kb on + strand, within MPMX20_00525 at 541.704 kb on + strand, within MPMX20_00525 at 541.704 kb on + strand, within MPMX20_00525 at 541.704 kb on + strand, within MPMX20_00525 at 541.704 kb on + strand, within MPMX20_00525 at 541.712 kb on - strand, within MPMX20_00525 at 541.712 kb on - strand, within MPMX20_00525 at 541.712 kb on - strand, within MPMX20_00525 at 541.712 kb on - strand, within MPMX20_00525 at 541.726 kb on + strand, within MPMX20_00525 at 541.734 kb on - strand, within MPMX20_00525 at 541.792 kb on - strand, within MPMX20_00525 at 541.809 kb on - strand, within MPMX20_00525 at 541.809 kb on - strand, within MPMX20_00525
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_glucose plate, 1:1 coculture with Enterobacter_TBS_079 remove 538,775 + MPMX20_00522 0.69 -1.4 538,778 + MPMX20_00522 0.70 -2.6 538,794 + MPMX20_00522 0.72 -0.9 538,841 + MPMX20_00522 0.78 +0.7 538,855 + MPMX20_00522 0.80 -1.2 538,910 - MPMX20_00522 0.87 -2.5 538,982 + +2.2 539,043 - -0.2 539,043 - -0.7 539,076 + -3.3 539,086 + +0.6 539,089 - -0.2 539,094 - MPMX20_00523 0.10 -0.1 539,097 - MPMX20_00523 0.11 +0.6 539,141 - MPMX20_00523 0.18 -1.3 539,143 + MPMX20_00523 0.18 -2.7 539,260 + MPMX20_00523 0.36 -1.1 539,296 - MPMX20_00523 0.42 +0.6 539,296 - MPMX20_00523 0.42 -1.2 539,449 - MPMX20_00523 0.66 +1.7 539,481 - MPMX20_00523 0.71 +2.4 539,570 - MPMX20_00523 0.85 +0.1 539,574 - MPMX20_00523 0.85 +0.6 539,643 - -1.4 539,644 - -1.0 539,650 + -1.6 539,654 - -1.9 539,694 - +1.3 539,694 - -1.5 539,723 + +1.5 539,723 + -0.7 539,723 + -0.2 539,731 - -0.6 539,731 - -0.5 539,756 - -1.2 539,761 - +2.1 539,841 - MPMX20_00524 0.11 -2.7 539,848 - MPMX20_00524 0.12 -1.9 539,854 - MPMX20_00524 0.13 +0.3 539,873 - MPMX20_00524 0.14 +0.1 539,873 - MPMX20_00524 0.14 -1.1 539,879 + MPMX20_00524 0.15 -0.7 539,923 - MPMX20_00524 0.19 +10.0 540,017 + MPMX20_00524 0.27 +0.7 540,035 + MPMX20_00524 0.29 +2.1 540,037 - MPMX20_00524 0.29 +0.2 540,048 + MPMX20_00524 0.30 -1.1 540,068 + MPMX20_00524 0.31 -0.4 540,076 - MPMX20_00524 0.32 +0.8 540,124 - MPMX20_00524 0.36 -0.5 540,195 + MPMX20_00524 0.43 +1.8 540,307 - MPMX20_00524 0.53 +0.2 540,309 + MPMX20_00524 0.53 -0.0 540,309 + MPMX20_00524 0.53 +0.1 540,309 + MPMX20_00524 0.53 -1.1 540,309 + MPMX20_00524 0.53 +2.6 540,314 + MPMX20_00524 0.53 -3.7 540,314 + MPMX20_00524 0.53 -0.7 540,317 - MPMX20_00524 0.53 -1.2 540,317 - MPMX20_00524 0.53 -0.0 540,317 - MPMX20_00524 0.53 +0.2 540,386 + MPMX20_00524 0.60 -0.4 540,479 - MPMX20_00524 0.68 +0.4 540,512 - MPMX20_00524 0.71 -0.3 540,678 + MPMX20_00524 0.85 -1.8 540,714 - MPMX20_00524 0.88 -0.5 540,791 + -2.6 540,797 + +0.1 540,817 - -0.7 540,817 - -2.3 540,824 + +2.0 540,827 - -0.7 540,827 - -1.2 540,853 + -0.5 540,873 + +0.8 540,874 + +1.2 540,975 - MPMX20_00525 0.13 -1.4 541,021 + MPMX20_00525 0.17 +1.4 541,029 - MPMX20_00525 0.18 +0.3 541,037 - MPMX20_00525 0.19 -2.0 541,037 - MPMX20_00525 0.19 +0.8 541,080 + MPMX20_00525 0.23 +1.6 541,244 - MPMX20_00525 0.37 +0.3 541,303 + MPMX20_00525 0.42 +1.3 541,305 + MPMX20_00525 0.43 -1.2 541,311 - MPMX20_00525 0.43 +1.4 541,311 - MPMX20_00525 0.43 -1.7 541,313 - MPMX20_00525 0.43 +0.9 541,334 - MPMX20_00525 0.45 +0.6 541,336 + MPMX20_00525 0.45 -1.2 541,336 + MPMX20_00525 0.45 +2.1 541,336 + MPMX20_00525 0.45 -0.2 541,344 - MPMX20_00525 0.46 -1.8 541,344 - MPMX20_00525 0.46 +0.9 541,344 - MPMX20_00525 0.46 -2.7 541,375 - MPMX20_00525 0.49 -0.9 541,429 - MPMX20_00525 0.54 +0.9 541,435 - MPMX20_00525 0.54 -0.1 541,586 + MPMX20_00525 0.68 +0.4 541,586 + MPMX20_00525 0.68 -1.7 541,594 - MPMX20_00525 0.68 -2.0 541,607 + MPMX20_00525 0.70 -3.3 541,630 + MPMX20_00525 0.72 -3.9 541,649 + MPMX20_00525 0.73 -1.4 541,671 + MPMX20_00525 0.75 -1.2 541,686 - MPMX20_00525 0.77 +1.5 541,704 + MPMX20_00525 0.78 +2.1 541,704 + MPMX20_00525 0.78 -2.4 541,704 + MPMX20_00525 0.78 +0.4 541,704 + MPMX20_00525 0.78 +4.1 541,704 + MPMX20_00525 0.78 -1.4 541,704 + MPMX20_00525 0.78 -0.2 541,704 + MPMX20_00525 0.78 +0.6 541,712 - MPMX20_00525 0.79 +0.3 541,712 - MPMX20_00525 0.79 -3.0 541,712 - MPMX20_00525 0.79 +1.1 541,712 - MPMX20_00525 0.79 -1.2 541,726 + MPMX20_00525 0.80 +1.6 541,734 - MPMX20_00525 0.81 -3.0 541,792 - MPMX20_00525 0.86 +1.9 541,809 - MPMX20_00525 0.88 -1.6 541,809 - MPMX20_00525 0.88 -2.9
Or see this region's nucleotide sequence