Experiment: pyrophosphate 1 mM phosphorus source
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS17170 and HMPREF1058_RS17165 are separated by 157 nucleotides HMPREF1058_RS17165 and HMPREF1058_RS17160 are separated by 13 nucleotides HMPREF1058_RS17160 and HMPREF1058_RS17155 are separated by 4 nucleotides HMPREF1058_RS17155 and HMPREF1058_RS17150 are separated by 14 nucleotides
HMPREF1058_RS17170: HMPREF1058_RS17170 - Crp/Fnr family transcriptional regulator, at 509,815 to 510,492
_RS17170
HMPREF1058_RS17165: HMPREF1058_RS17165 - M3 family metallopeptidase, at 510,650 to 512,692
_RS17165
HMPREF1058_RS17160: HMPREF1058_RS17160 - DUF4847 family protein, at 512,706 to 513,200
_RS17160
HMPREF1058_RS17155: HMPREF1058_RS17155 - dCMP deaminase family protein, at 513,205 to 513,639
_RS17155
HMPREF1058_RS17150: HMPREF1058_RS17150 - S41 family peptidase, at 513,654 to 515,402
_RS17150
Position (kb) 510
511
512
513 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 509.655 kb on - strand at 509.655 kb on - strand at 509.657 kb on - strand at 509.682 kb on + strand at 509.683 kb on - strand at 509.745 kb on + strand at 509.773 kb on - strand at 509.781 kb on - strand at 509.791 kb on - strand at 509.824 kb on - strand at 509.830 kb on + strand at 509.831 kb on - strand at 509.835 kb on + strand at 509.903 kb on + strand, within HMPREF1058_RS17170 at 509.904 kb on - strand, within HMPREF1058_RS17170 at 509.984 kb on + strand, within HMPREF1058_RS17170 at 509.996 kb on + strand, within HMPREF1058_RS17170 at 510.028 kb on + strand, within HMPREF1058_RS17170 at 510.035 kb on + strand at 510.065 kb on + strand, within HMPREF1058_RS17170 at 510.100 kb on + strand, within HMPREF1058_RS17170 at 510.102 kb on + strand, within HMPREF1058_RS17170 at 510.102 kb on + strand, within HMPREF1058_RS17170 at 510.124 kb on - strand, within HMPREF1058_RS17170 at 510.124 kb on - strand, within HMPREF1058_RS17170 at 510.124 kb on - strand, within HMPREF1058_RS17170 at 510.137 kb on + strand, within HMPREF1058_RS17170 at 510.138 kb on - strand, within HMPREF1058_RS17170 at 510.138 kb on - strand, within HMPREF1058_RS17170 at 510.155 kb on + strand, within HMPREF1058_RS17170 at 510.155 kb on + strand, within HMPREF1058_RS17170 at 510.156 kb on - strand, within HMPREF1058_RS17170 at 510.156 kb on - strand, within HMPREF1058_RS17170 at 510.198 kb on + strand, within HMPREF1058_RS17170 at 510.207 kb on - strand, within HMPREF1058_RS17170 at 510.219 kb on + strand, within HMPREF1058_RS17170 at 510.222 kb on + strand, within HMPREF1058_RS17170 at 510.222 kb on + strand, within HMPREF1058_RS17170 at 510.263 kb on + strand, within HMPREF1058_RS17170 at 510.263 kb on + strand, within HMPREF1058_RS17170 at 510.264 kb on - strand at 510.428 kb on + strand at 510.499 kb on - strand at 510.523 kb on + strand at 510.524 kb on - strand at 510.563 kb on - strand at 510.567 kb on - strand at 510.570 kb on - strand at 510.572 kb on - strand at 510.587 kb on + strand at 510.644 kb on - strand at 510.718 kb on + strand at 510.719 kb on - strand at 510.719 kb on - strand at 510.726 kb on + strand at 510.734 kb on + strand at 510.867 kb on + strand, within HMPREF1058_RS17165 at 511.008 kb on + strand, within HMPREF1058_RS17165 at 511.010 kb on + strand, within HMPREF1058_RS17165 at 511.070 kb on + strand, within HMPREF1058_RS17165 at 511.071 kb on - strand, within HMPREF1058_RS17165 at 511.146 kb on + strand, within HMPREF1058_RS17165 at 511.152 kb on + strand, within HMPREF1058_RS17165 at 511.152 kb on + strand, within HMPREF1058_RS17165 at 511.152 kb on + strand, within HMPREF1058_RS17165 at 511.340 kb on + strand, within HMPREF1058_RS17165 at 511.373 kb on + strand, within HMPREF1058_RS17165 at 511.373 kb on + strand, within HMPREF1058_RS17165 at 511.382 kb on + strand, within HMPREF1058_RS17165 at 511.385 kb on - strand, within HMPREF1058_RS17165 at 511.385 kb on - strand, within HMPREF1058_RS17165 at 511.388 kb on - strand, within HMPREF1058_RS17165 at 511.470 kb on + strand, within HMPREF1058_RS17165 at 511.473 kb on + strand, within HMPREF1058_RS17165 at 511.539 kb on - strand, within HMPREF1058_RS17165 at 511.568 kb on + strand, within HMPREF1058_RS17165 at 511.569 kb on - strand, within HMPREF1058_RS17165 at 511.664 kb on + strand, within HMPREF1058_RS17165 at 511.667 kb on + strand, within HMPREF1058_RS17165 at 511.725 kb on - strand, within HMPREF1058_RS17165 at 511.779 kb on - strand, within HMPREF1058_RS17165 at 511.839 kb on - strand, within HMPREF1058_RS17165 at 511.876 kb on - strand, within HMPREF1058_RS17165 at 511.880 kb on + strand, within HMPREF1058_RS17165 at 511.950 kb on + strand, within HMPREF1058_RS17165 at 512.130 kb on + strand, within HMPREF1058_RS17165 at 512.130 kb on + strand, within HMPREF1058_RS17165 at 512.130 kb on + strand, within HMPREF1058_RS17165 at 512.130 kb on + strand, within HMPREF1058_RS17165 at 512.130 kb on + strand, within HMPREF1058_RS17165 at 512.131 kb on - strand, within HMPREF1058_RS17165 at 512.288 kb on + strand at 512.336 kb on + strand, within HMPREF1058_RS17165 at 512.337 kb on - strand, within HMPREF1058_RS17165 at 512.376 kb on - strand, within HMPREF1058_RS17165 at 512.406 kb on + strand, within HMPREF1058_RS17165 at 512.452 kb on + strand, within HMPREF1058_RS17165 at 512.452 kb on + strand, within HMPREF1058_RS17165 at 512.625 kb on - strand at 512.714 kb on - strand at 512.714 kb on - strand at 512.727 kb on + strand at 512.729 kb on + strand at 512.729 kb on + strand at 512.729 kb on + strand at 512.730 kb on - strand at 512.731 kb on + strand at 512.734 kb on + strand at 512.753 kb on - strand at 512.865 kb on + strand, within HMPREF1058_RS17160 at 512.866 kb on - strand, within HMPREF1058_RS17160 at 512.866 kb on - strand, within HMPREF1058_RS17160 at 512.966 kb on + strand, within HMPREF1058_RS17160 at 513.112 kb on + strand, within HMPREF1058_RS17160 at 513.145 kb on + strand, within HMPREF1058_RS17160 at 513.185 kb on + strand at 513.185 kb on + strand at 513.185 kb on + strand at 513.186 kb on - strand at 513.186 kb on - strand at 513.203 kb on + strand at 513.204 kb on - strand at 513.247 kb on + strand at 513.264 kb on + strand, within HMPREF1058_RS17155 at 513.283 kb on + strand, within HMPREF1058_RS17155 at 513.347 kb on - strand, within HMPREF1058_RS17155 at 513.414 kb on - strand, within HMPREF1058_RS17155 at 513.446 kb on + strand, within HMPREF1058_RS17155 at 513.484 kb on + strand, within HMPREF1058_RS17155 at 513.485 kb on - strand, within HMPREF1058_RS17155 at 513.485 kb on - strand, within HMPREF1058_RS17155 at 513.506 kb on + strand, within HMPREF1058_RS17155 at 513.507 kb on - strand, within HMPREF1058_RS17155 at 513.607 kb on - strand at 513.648 kb on - strand at 513.653 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 1 mM phosphorus source remove 509,655 - -1.2 509,655 - -0.3 509,657 - +0.5 509,682 + -0.3 509,683 - -0.2 509,745 + +0.9 509,773 - -1.7 509,781 - -1.2 509,791 - +1.9 509,824 - -2.5 509,830 + -2.0 509,831 - -0.7 509,835 + +0.2 509,903 + HMPREF1058_RS17170 0.13 +0.1 509,904 - HMPREF1058_RS17170 0.13 -0.4 509,984 + HMPREF1058_RS17170 0.25 -3.2 509,996 + HMPREF1058_RS17170 0.27 -1.1 510,028 + HMPREF1058_RS17170 0.31 -1.2 510,035 + -0.5 510,065 + HMPREF1058_RS17170 0.37 -1.1 510,100 + HMPREF1058_RS17170 0.42 +1.3 510,102 + HMPREF1058_RS17170 0.42 -0.8 510,102 + HMPREF1058_RS17170 0.42 -2.0 510,124 - HMPREF1058_RS17170 0.46 -3.3 510,124 - HMPREF1058_RS17170 0.46 -0.4 510,124 - HMPREF1058_RS17170 0.46 +0.9 510,137 + HMPREF1058_RS17170 0.47 -1.6 510,138 - HMPREF1058_RS17170 0.48 +2.9 510,138 - HMPREF1058_RS17170 0.48 -0.7 510,155 + HMPREF1058_RS17170 0.50 -2.0 510,155 + HMPREF1058_RS17170 0.50 -2.7 510,156 - HMPREF1058_RS17170 0.50 +0.9 510,156 - HMPREF1058_RS17170 0.50 -1.7 510,198 + HMPREF1058_RS17170 0.56 -1.7 510,207 - HMPREF1058_RS17170 0.58 -0.6 510,219 + HMPREF1058_RS17170 0.60 -0.9 510,222 + HMPREF1058_RS17170 0.60 -1.6 510,222 + HMPREF1058_RS17170 0.60 -1.8 510,263 + HMPREF1058_RS17170 0.66 -1.9 510,263 + HMPREF1058_RS17170 0.66 -0.1 510,264 - +0.1 510,428 + -2.0 510,499 - +0.8 510,523 + -0.6 510,524 - -1.5 510,563 - +3.6 510,567 - -0.7 510,570 - +0.3 510,572 - +3.8 510,587 + +0.6 510,644 - -0.4 510,718 + +0.8 510,719 - +0.3 510,719 - -0.2 510,726 + +0.6 510,734 + +2.0 510,867 + HMPREF1058_RS17165 0.11 +3.5 511,008 + HMPREF1058_RS17165 0.18 +4.8 511,010 + HMPREF1058_RS17165 0.18 +2.8 511,070 + HMPREF1058_RS17165 0.21 +3.6 511,071 - HMPREF1058_RS17165 0.21 +0.3 511,146 + HMPREF1058_RS17165 0.24 +2.8 511,152 + HMPREF1058_RS17165 0.25 +3.3 511,152 + HMPREF1058_RS17165 0.25 +2.7 511,152 + HMPREF1058_RS17165 0.25 +3.1 511,340 + HMPREF1058_RS17165 0.34 +6.3 511,373 + HMPREF1058_RS17165 0.35 +2.5 511,373 + HMPREF1058_RS17165 0.35 +3.9 511,382 + HMPREF1058_RS17165 0.36 +3.7 511,385 - HMPREF1058_RS17165 0.36 +0.9 511,385 - HMPREF1058_RS17165 0.36 -1.9 511,388 - HMPREF1058_RS17165 0.36 +2.9 511,470 + HMPREF1058_RS17165 0.40 +3.0 511,473 + HMPREF1058_RS17165 0.40 +3.9 511,539 - HMPREF1058_RS17165 0.44 -0.1 511,568 + HMPREF1058_RS17165 0.45 +4.4 511,569 - HMPREF1058_RS17165 0.45 +0.3 511,664 + HMPREF1058_RS17165 0.50 +3.0 511,667 + HMPREF1058_RS17165 0.50 +3.1 511,725 - HMPREF1058_RS17165 0.53 +0.9 511,779 - HMPREF1058_RS17165 0.55 -0.5 511,839 - HMPREF1058_RS17165 0.58 -0.4 511,876 - HMPREF1058_RS17165 0.60 -2.4 511,880 + HMPREF1058_RS17165 0.60 +4.5 511,950 + HMPREF1058_RS17165 0.64 +2.9 512,130 + HMPREF1058_RS17165 0.72 +0.1 512,130 + HMPREF1058_RS17165 0.72 -1.4 512,130 + HMPREF1058_RS17165 0.72 +0.2 512,130 + HMPREF1058_RS17165 0.72 -0.1 512,130 + HMPREF1058_RS17165 0.72 -0.9 512,131 - HMPREF1058_RS17165 0.72 -1.0 512,288 + -0.4 512,336 + HMPREF1058_RS17165 0.83 -0.1 512,337 - HMPREF1058_RS17165 0.83 +0.1 512,376 - HMPREF1058_RS17165 0.84 -0.4 512,406 + HMPREF1058_RS17165 0.86 -0.0 512,452 + HMPREF1058_RS17165 0.88 -0.1 512,452 + HMPREF1058_RS17165 0.88 +1.0 512,625 - -1.9 512,714 - +0.3 512,714 - -2.3 512,727 + +3.3 512,729 + +4.3 512,729 + +2.8 512,729 + +1.0 512,730 - -0.5 512,731 + +4.7 512,734 + +4.2 512,753 - -0.2 512,865 + HMPREF1058_RS17160 0.32 +4.1 512,866 - HMPREF1058_RS17160 0.32 -0.2 512,866 - HMPREF1058_RS17160 0.32 -1.2 512,966 + HMPREF1058_RS17160 0.53 +4.0 513,112 + HMPREF1058_RS17160 0.82 +4.8 513,145 + HMPREF1058_RS17160 0.89 +4.2 513,185 + +1.8 513,185 + +0.8 513,185 + +1.0 513,186 - -0.5 513,186 - +0.1 513,203 + -1.8 513,204 - +0.5 513,247 + -0.5 513,264 + HMPREF1058_RS17155 0.14 -1.0 513,283 + HMPREF1058_RS17155 0.18 -2.3 513,347 - HMPREF1058_RS17155 0.33 -0.2 513,414 - HMPREF1058_RS17155 0.48 -1.9 513,446 + HMPREF1058_RS17155 0.55 +0.9 513,484 + HMPREF1058_RS17155 0.64 -3.2 513,485 - HMPREF1058_RS17155 0.64 -2.8 513,485 - HMPREF1058_RS17155 0.64 +0.9 513,506 + HMPREF1058_RS17155 0.69 -0.8 513,507 - HMPREF1058_RS17155 0.69 -2.2 513,607 - -1.1 513,648 - +0.3 513,653 + +1.3
Or see this region's nucleotide sequence