Experiment: pyrophosphate 1 mM phosphorus source
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS07050 and HMPREF1058_RS07045 are separated by 23 nucleotides HMPREF1058_RS07045 and HMPREF1058_RS07040 are separated by 72 nucleotides HMPREF1058_RS07040 and HMPREF1058_RS07035 are separated by 80 nucleotides
HMPREF1058_RS07050: HMPREF1058_RS07050 - Holliday junction resolvase RuvX, at 327,732 to 328,151
_RS07050
HMPREF1058_RS07045: HMPREF1058_RS07045 - peptide deformylase, at 328,175 to 328,729
_RS07045
HMPREF1058_RS07040: HMPREF1058_RS07040 - tetratricopeptide repeat protein, at 328,802 to 330,811
_RS07040
HMPREF1058_RS07035: HMPREF1058_RS07035 - threonine--tRNA ligase, at 330,892 to 332,832
_RS07035
Position (kb) 328
329
330
331 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 328.143 kb on + strand at 328.749 kb on + strand at 328.750 kb on - strand at 328.750 kb on - strand at 328.793 kb on - strand at 328.793 kb on - strand at 328.820 kb on + strand at 328.821 kb on - strand at 328.823 kb on - strand at 328.823 kb on - strand at 328.827 kb on + strand at 328.827 kb on + strand at 328.828 kb on - strand at 328.830 kb on + strand at 328.863 kb on + strand at 328.904 kb on + strand at 328.905 kb on - strand at 328.916 kb on + strand at 328.917 kb on - strand at 328.917 kb on - strand at 328.964 kb on + strand at 328.985 kb on + strand at 329.050 kb on + strand, within HMPREF1058_RS07040 at 329.073 kb on + strand, within HMPREF1058_RS07040 at 329.116 kb on + strand, within HMPREF1058_RS07040 at 329.133 kb on + strand, within HMPREF1058_RS07040 at 329.143 kb on + strand, within HMPREF1058_RS07040 at 329.144 kb on - strand, within HMPREF1058_RS07040 at 329.144 kb on - strand, within HMPREF1058_RS07040 at 329.176 kb on - strand, within HMPREF1058_RS07040 at 329.176 kb on - strand, within HMPREF1058_RS07040 at 329.176 kb on - strand, within HMPREF1058_RS07040 at 329.188 kb on + strand, within HMPREF1058_RS07040 at 329.188 kb on + strand, within HMPREF1058_RS07040 at 329.188 kb on + strand, within HMPREF1058_RS07040 at 329.222 kb on + strand, within HMPREF1058_RS07040 at 329.222 kb on + strand, within HMPREF1058_RS07040 at 329.275 kb on + strand, within HMPREF1058_RS07040 at 329.276 kb on - strand, within HMPREF1058_RS07040 at 329.324 kb on - strand, within HMPREF1058_RS07040 at 329.383 kb on + strand, within HMPREF1058_RS07040 at 329.383 kb on + strand, within HMPREF1058_RS07040 at 329.384 kb on - strand, within HMPREF1058_RS07040 at 329.427 kb on - strand, within HMPREF1058_RS07040 at 329.429 kb on - strand, within HMPREF1058_RS07040 at 329.492 kb on - strand, within HMPREF1058_RS07040 at 329.623 kb on - strand, within HMPREF1058_RS07040 at 329.732 kb on + strand, within HMPREF1058_RS07040 at 329.732 kb on + strand, within HMPREF1058_RS07040 at 329.734 kb on + strand, within HMPREF1058_RS07040 at 329.735 kb on - strand, within HMPREF1058_RS07040 at 329.827 kb on - strand, within HMPREF1058_RS07040 at 329.935 kb on + strand, within HMPREF1058_RS07040 at 329.938 kb on + strand, within HMPREF1058_RS07040 at 329.938 kb on + strand, within HMPREF1058_RS07040 at 329.975 kb on + strand, within HMPREF1058_RS07040 at 329.980 kb on + strand, within HMPREF1058_RS07040 at 329.981 kb on - strand, within HMPREF1058_RS07040 at 330.021 kb on - strand, within HMPREF1058_RS07040 at 330.095 kb on + strand, within HMPREF1058_RS07040 at 330.096 kb on - strand, within HMPREF1058_RS07040 at 330.140 kb on - strand, within HMPREF1058_RS07040 at 330.172 kb on - strand, within HMPREF1058_RS07040 at 330.229 kb on + strand, within HMPREF1058_RS07040 at 330.374 kb on - strand, within HMPREF1058_RS07040 at 330.374 kb on - strand, within HMPREF1058_RS07040 at 330.425 kb on + strand, within HMPREF1058_RS07040 at 330.427 kb on + strand, within HMPREF1058_RS07040 at 330.569 kb on + strand, within HMPREF1058_RS07040 at 330.576 kb on - strand, within HMPREF1058_RS07040 at 330.579 kb on - strand, within HMPREF1058_RS07040 at 330.580 kb on + strand, within HMPREF1058_RS07040 at 330.581 kb on - strand, within HMPREF1058_RS07040 at 330.584 kb on - strand, within HMPREF1058_RS07040 at 330.615 kb on - strand at 330.636 kb on - strand at 330.677 kb on + strand at 330.728 kb on - strand at 330.779 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction pyrophosphate 1 mM phosphorus source remove 328,143 + -0.5 328,749 + -0.0 328,750 - -2.8 328,750 - -0.1 328,793 - -4.1 328,793 - -1.5 328,820 + -1.2 328,821 - -4.2 328,823 - -2.2 328,823 - -3.9 328,827 + -2.4 328,827 + -1.9 328,828 - -2.0 328,830 + -3.3 328,863 + -1.2 328,904 + -1.0 328,905 - -2.8 328,916 + -3.3 328,917 - -2.2 328,917 - -3.3 328,964 + -1.0 328,985 + -0.2 329,050 + HMPREF1058_RS07040 0.12 -2.6 329,073 + HMPREF1058_RS07040 0.13 -2.2 329,116 + HMPREF1058_RS07040 0.16 -2.1 329,133 + HMPREF1058_RS07040 0.16 +1.9 329,143 + HMPREF1058_RS07040 0.17 -1.6 329,144 - HMPREF1058_RS07040 0.17 -1.0 329,144 - HMPREF1058_RS07040 0.17 -2.0 329,176 - HMPREF1058_RS07040 0.19 -1.1 329,176 - HMPREF1058_RS07040 0.19 -2.9 329,176 - HMPREF1058_RS07040 0.19 -2.3 329,188 + HMPREF1058_RS07040 0.19 -2.4 329,188 + HMPREF1058_RS07040 0.19 -3.4 329,188 + HMPREF1058_RS07040 0.19 -1.2 329,222 + HMPREF1058_RS07040 0.21 +0.1 329,222 + HMPREF1058_RS07040 0.21 +0.9 329,275 + HMPREF1058_RS07040 0.24 -2.6 329,276 - HMPREF1058_RS07040 0.24 -3.0 329,324 - HMPREF1058_RS07040 0.26 -2.8 329,383 + HMPREF1058_RS07040 0.29 -1.1 329,383 + HMPREF1058_RS07040 0.29 -1.1 329,384 - HMPREF1058_RS07040 0.29 -0.8 329,427 - HMPREF1058_RS07040 0.31 -3.2 329,429 - HMPREF1058_RS07040 0.31 -3.5 329,492 - HMPREF1058_RS07040 0.34 +0.4 329,623 - HMPREF1058_RS07040 0.41 -1.9 329,732 + HMPREF1058_RS07040 0.46 +0.2 329,732 + HMPREF1058_RS07040 0.46 -2.2 329,734 + HMPREF1058_RS07040 0.46 -2.9 329,735 - HMPREF1058_RS07040 0.46 -3.6 329,827 - HMPREF1058_RS07040 0.51 -1.4 329,935 + HMPREF1058_RS07040 0.56 -1.7 329,938 + HMPREF1058_RS07040 0.57 -0.7 329,938 + HMPREF1058_RS07040 0.57 -3.7 329,975 + HMPREF1058_RS07040 0.58 +0.6 329,980 + HMPREF1058_RS07040 0.59 -2.7 329,981 - HMPREF1058_RS07040 0.59 -4.4 330,021 - HMPREF1058_RS07040 0.61 -2.9 330,095 + HMPREF1058_RS07040 0.64 -0.8 330,096 - HMPREF1058_RS07040 0.64 -2.5 330,140 - HMPREF1058_RS07040 0.67 +0.0 330,172 - HMPREF1058_RS07040 0.68 -1.4 330,229 + HMPREF1058_RS07040 0.71 -2.3 330,374 - HMPREF1058_RS07040 0.78 -1.1 330,374 - HMPREF1058_RS07040 0.78 -2.4 330,425 + HMPREF1058_RS07040 0.81 -2.5 330,427 + HMPREF1058_RS07040 0.81 -2.6 330,569 + HMPREF1058_RS07040 0.88 -0.1 330,576 - HMPREF1058_RS07040 0.88 -2.6 330,579 - HMPREF1058_RS07040 0.88 -1.6 330,580 + HMPREF1058_RS07040 0.88 -1.1 330,581 - HMPREF1058_RS07040 0.89 -1.7 330,584 - HMPREF1058_RS07040 0.89 -0.7 330,615 - -1.4 330,636 - -1.1 330,677 + +0.6 330,728 - +0.8 330,779 - -0.5
Or see this region's nucleotide sequence