Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS17410 and HMPREF1058_RS17405 are separated by 49 nucleotides HMPREF1058_RS17405 and HMPREF1058_RS17400 are separated by 8 nucleotides HMPREF1058_RS17400 and HMPREF1058_RS17395 are separated by 108 nucleotides HMPREF1058_RS17395 and HMPREF1058_RS21415 overlap by 4 nucleotides HMPREF1058_RS21415 and HMPREF1058_RS17390 are separated by 40 nucleotides
HMPREF1058_RS17410: HMPREF1058_RS17410 - undecaprenyl-phosphate glucose phosphotransferase, at 450,190 to 451,593
_RS17410
HMPREF1058_RS17405: HMPREF1058_RS17405 - polysaccharide export protein, at 451,643 to 452,434
_RS17405
HMPREF1058_RS17400: HMPREF1058_RS17400 - tyrosine-protein kinase, at 452,443 to 454,854
_RS17400
HMPREF1058_RS17395: HMPREF1058_RS17395 - N-acetylmuramoyl-L-alanine amidase, at 454,963 to 455,412
_RS17395
HMPREF1058_RS21415: HMPREF1058_RS21415 - smalltalk protein, at 455,409 to 455,510
_RS21415
HMPREF1058_RS17390: HMPREF1058_RS17390 - HU family DNA-binding protein, at 455,551 to 455,979
_RS17390
Position (kb) 452
453
454
455 Strain fitness (log2 ratio) -2
-1
0
1
2 at 451.499 kb on + strand at 451.499 kb on + strand at 451.504 kb on + strand at 451.539 kb on + strand at 451.540 kb on - strand at 451.542 kb on + strand at 451.624 kb on + strand at 451.625 kb on - strand at 451.740 kb on - strand, within HMPREF1058_RS17405 at 451.772 kb on + strand, within HMPREF1058_RS17405 at 451.773 kb on - strand, within HMPREF1058_RS17405 at 451.919 kb on + strand, within HMPREF1058_RS17405 at 452.030 kb on + strand, within HMPREF1058_RS17405 at 452.058 kb on + strand, within HMPREF1058_RS17405 at 452.059 kb on - strand, within HMPREF1058_RS17405 at 452.140 kb on + strand, within HMPREF1058_RS17405 at 452.141 kb on - strand, within HMPREF1058_RS17405 at 452.178 kb on + strand, within HMPREF1058_RS17405 at 452.179 kb on - strand, within HMPREF1058_RS17405 at 452.285 kb on + strand, within HMPREF1058_RS17405 at 452.286 kb on - strand, within HMPREF1058_RS17405 at 452.286 kb on - strand, within HMPREF1058_RS17405 at 452.351 kb on - strand, within HMPREF1058_RS17405 at 452.443 kb on - strand at 452.553 kb on + strand at 452.584 kb on + strand at 452.584 kb on + strand at 452.963 kb on - strand, within HMPREF1058_RS17400 at 453.132 kb on + strand, within HMPREF1058_RS17400 at 453.133 kb on - strand, within HMPREF1058_RS17400 at 453.874 kb on - strand, within HMPREF1058_RS17400 at 453.950 kb on - strand, within HMPREF1058_RS17400 at 453.998 kb on - strand, within HMPREF1058_RS17400 at 454.000 kb on - strand, within HMPREF1058_RS17400 at 454.080 kb on - strand, within HMPREF1058_RS17400 at 454.080 kb on - strand, within HMPREF1058_RS17400 at 454.118 kb on + strand, within HMPREF1058_RS17400 at 454.118 kb on + strand, within HMPREF1058_RS17400 at 454.166 kb on + strand, within HMPREF1058_RS17400 at 454.178 kb on + strand, within HMPREF1058_RS17400 at 454.183 kb on + strand, within HMPREF1058_RS17400 at 454.183 kb on + strand, within HMPREF1058_RS17400 at 454.183 kb on + strand at 454.184 kb on - strand, within HMPREF1058_RS17400 at 454.184 kb on - strand, within HMPREF1058_RS17400 at 454.186 kb on - strand, within HMPREF1058_RS17400 at 454.186 kb on - strand, within HMPREF1058_RS17400 at 454.470 kb on + strand, within HMPREF1058_RS17400 at 454.471 kb on - strand, within HMPREF1058_RS17400 at 454.550 kb on - strand, within HMPREF1058_RS17400 at 454.551 kb on + strand, within HMPREF1058_RS17400 at 454.627 kb on + strand at 454.627 kb on + strand at 454.627 kb on + strand at 454.627 kb on + strand at 454.628 kb on - strand at 454.645 kb on + strand at 454.710 kb on - strand at 454.710 kb on - strand at 454.714 kb on - strand at 454.778 kb on - strand at 455.060 kb on - strand, within HMPREF1058_RS17395 at 455.192 kb on + strand, within HMPREF1058_RS17395 at 455.217 kb on + strand, within HMPREF1058_RS17395 at 455.501 kb on + strand at 455.553 kb on - strand at 455.687 kb on + strand, within HMPREF1058_RS17390 at 455.687 kb on + strand, within HMPREF1058_RS17390 at 455.688 kb on - strand, within HMPREF1058_RS17390 at 455.688 kb on - strand, within HMPREF1058_RS17390 at 455.688 kb on - strand, within HMPREF1058_RS17390 at 455.756 kb on - strand, within HMPREF1058_RS17390
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 451,499 + +1.8 451,499 + -1.0 451,504 + +2.3 451,539 + -1.3 451,540 - -0.1 451,542 + +1.3 451,624 + -0.2 451,625 - -0.3 451,740 - HMPREF1058_RS17405 0.12 -0.8 451,772 + HMPREF1058_RS17405 0.16 +0.4 451,773 - HMPREF1058_RS17405 0.16 -0.6 451,919 + HMPREF1058_RS17405 0.35 -2.1 452,030 + HMPREF1058_RS17405 0.49 +0.7 452,058 + HMPREF1058_RS17405 0.52 +0.9 452,059 - HMPREF1058_RS17405 0.53 +0.7 452,140 + HMPREF1058_RS17405 0.63 +0.1 452,141 - HMPREF1058_RS17405 0.63 +0.5 452,178 + HMPREF1058_RS17405 0.68 +0.3 452,179 - HMPREF1058_RS17405 0.68 +0.3 452,285 + HMPREF1058_RS17405 0.81 +1.7 452,286 - HMPREF1058_RS17405 0.81 -0.3 452,286 - HMPREF1058_RS17405 0.81 -0.0 452,351 - HMPREF1058_RS17405 0.89 -0.8 452,443 - -1.4 452,553 + -0.8 452,584 + -0.1 452,584 + -0.7 452,963 - HMPREF1058_RS17400 0.22 -1.1 453,132 + HMPREF1058_RS17400 0.29 -1.1 453,133 - HMPREF1058_RS17400 0.29 -2.0 453,874 - HMPREF1058_RS17400 0.59 +2.3 453,950 - HMPREF1058_RS17400 0.62 -0.0 453,998 - HMPREF1058_RS17400 0.64 -1.6 454,000 - HMPREF1058_RS17400 0.65 -0.9 454,080 - HMPREF1058_RS17400 0.68 -0.2 454,080 - HMPREF1058_RS17400 0.68 -2.3 454,118 + HMPREF1058_RS17400 0.69 +1.0 454,118 + HMPREF1058_RS17400 0.69 +0.5 454,166 + HMPREF1058_RS17400 0.71 -0.3 454,178 + HMPREF1058_RS17400 0.72 +0.2 454,183 + HMPREF1058_RS17400 0.72 -1.7 454,183 + HMPREF1058_RS17400 0.72 -1.9 454,183 + +0.7 454,184 - HMPREF1058_RS17400 0.72 -1.9 454,184 - HMPREF1058_RS17400 0.72 +0.6 454,186 - HMPREF1058_RS17400 0.72 -0.3 454,186 - HMPREF1058_RS17400 0.72 +0.1 454,470 + HMPREF1058_RS17400 0.84 +0.3 454,471 - HMPREF1058_RS17400 0.84 -0.0 454,550 - HMPREF1058_RS17400 0.87 +0.6 454,551 + HMPREF1058_RS17400 0.87 -0.7 454,627 + +0.8 454,627 + -0.1 454,627 + +0.3 454,627 + -2.2 454,628 - -0.0 454,645 + -2.5 454,710 - -0.6 454,710 - +0.8 454,714 - -0.7 454,778 - -0.6 455,060 - HMPREF1058_RS17395 0.22 +0.3 455,192 + HMPREF1058_RS17395 0.51 -0.7 455,217 + HMPREF1058_RS17395 0.56 -1.3 455,501 + +0.3 455,553 - +0.8 455,687 + HMPREF1058_RS17390 0.32 +2.1 455,687 + HMPREF1058_RS17390 0.32 +0.7 455,688 - HMPREF1058_RS17390 0.32 +1.3 455,688 - HMPREF1058_RS17390 0.32 +1.8 455,688 - HMPREF1058_RS17390 0.32 +0.5 455,756 - HMPREF1058_RS17390 0.48 -0.7
Or see this region's nucleotide sequence