Experiment: Vitafiber 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS14225 and HMPREF1058_RS14220 are separated by 81 nucleotides HMPREF1058_RS14220 and HMPREF1058_RS14215 are separated by 185 nucleotides HMPREF1058_RS14215 and HMPREF1058_RS14210 overlap by 8 nucleotides
HMPREF1058_RS14225: HMPREF1058_RS14225 - polyprenyl synthetase family protein, at 1,253,074 to 1,254,051
_RS14225
HMPREF1058_RS14220: HMPREF1058_RS14220 - energy transducer TonB, at 1,254,133 to 1,254,816
_RS14220
HMPREF1058_RS14215: HMPREF1058_RS14215 - (d)CMP kinase, at 1,255,002 to 1,255,691
_RS14215
HMPREF1058_RS14210: HMPREF1058_RS14210 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase, at 1,255,684 to 1,256,550
_RS14210
Position (kb)
1254
1255 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1253.145 kb on + strand at 1253.172 kb on + strand, within HMPREF1058_RS14225 at 1253.221 kb on - strand, within HMPREF1058_RS14225 at 1253.272 kb on - strand, within HMPREF1058_RS14225 at 1253.375 kb on - strand, within HMPREF1058_RS14225 at 1253.405 kb on + strand, within HMPREF1058_RS14225 at 1253.467 kb on + strand, within HMPREF1058_RS14225 at 1253.467 kb on + strand, within HMPREF1058_RS14225 at 1253.468 kb on - strand, within HMPREF1058_RS14225 at 1253.503 kb on + strand, within HMPREF1058_RS14225 at 1253.655 kb on + strand, within HMPREF1058_RS14225 at 1253.676 kb on - strand, within HMPREF1058_RS14225 at 1253.676 kb on - strand, within HMPREF1058_RS14225 at 1253.773 kb on - strand, within HMPREF1058_RS14225 at 1253.827 kb on - strand, within HMPREF1058_RS14225 at 1253.827 kb on - strand, within HMPREF1058_RS14225 at 1253.833 kb on - strand, within HMPREF1058_RS14225 at 1253.846 kb on - strand, within HMPREF1058_RS14225 at 1253.846 kb on - strand, within HMPREF1058_RS14225 at 1253.846 kb on - strand, within HMPREF1058_RS14225 at 1253.952 kb on - strand, within HMPREF1058_RS14225 at 1253.952 kb on - strand, within HMPREF1058_RS14225 at 1253.956 kb on + strand at 1253.967 kb on - strand at 1254.013 kb on - strand at 1254.013 kb on - strand at 1254.013 kb on - strand at 1254.114 kb on - strand at 1254.114 kb on - strand at 1254.118 kb on + strand at 1254.118 kb on + strand at 1254.162 kb on + strand at 1254.176 kb on - strand at 1254.260 kb on - strand, within HMPREF1058_RS14220 at 1254.301 kb on - strand, within HMPREF1058_RS14220 at 1254.328 kb on - strand, within HMPREF1058_RS14220 at 1254.367 kb on - strand, within HMPREF1058_RS14220 at 1254.488 kb on + strand, within HMPREF1058_RS14220 at 1254.549 kb on + strand, within HMPREF1058_RS14220 at 1254.550 kb on - strand, within HMPREF1058_RS14220 at 1254.584 kb on - strand, within HMPREF1058_RS14220 at 1254.589 kb on - strand, within HMPREF1058_RS14220 at 1254.658 kb on + strand, within HMPREF1058_RS14220 at 1254.673 kb on - strand at 1254.750 kb on - strand at 1254.816 kb on + strand at 1254.860 kb on + strand at 1254.941 kb on + strand at 1254.941 kb on + strand at 1254.988 kb on + strand at 1254.997 kb on + strand at 1254.997 kb on + strand at 1254.999 kb on + strand at 1254.999 kb on + strand at 1255.092 kb on + strand, within HMPREF1058_RS14215 at 1255.129 kb on + strand, within HMPREF1058_RS14215 at 1255.131 kb on + strand, within HMPREF1058_RS14215 at 1255.133 kb on + strand, within HMPREF1058_RS14215 at 1255.133 kb on + strand, within HMPREF1058_RS14215 at 1255.133 kb on + strand, within HMPREF1058_RS14215 at 1255.208 kb on + strand, within HMPREF1058_RS14215 at 1255.208 kb on + strand, within HMPREF1058_RS14215 at 1255.521 kb on + strand, within HMPREF1058_RS14215 at 1255.694 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vitafiber 10 mg/mL (C) remove 1,253,145 + -0.7 1,253,172 + HMPREF1058_RS14225 0.10 -2.0 1,253,221 - HMPREF1058_RS14225 0.15 +0.1 1,253,272 - HMPREF1058_RS14225 0.20 -0.9 1,253,375 - HMPREF1058_RS14225 0.31 +0.7 1,253,405 + HMPREF1058_RS14225 0.34 -2.0 1,253,467 + HMPREF1058_RS14225 0.40 -0.2 1,253,467 + HMPREF1058_RS14225 0.40 +1.2 1,253,468 - HMPREF1058_RS14225 0.40 -2.9 1,253,503 + HMPREF1058_RS14225 0.44 -1.1 1,253,655 + HMPREF1058_RS14225 0.59 -2.4 1,253,676 - HMPREF1058_RS14225 0.62 -2.3 1,253,676 - HMPREF1058_RS14225 0.62 -2.2 1,253,773 - HMPREF1058_RS14225 0.71 -0.5 1,253,827 - HMPREF1058_RS14225 0.77 -1.0 1,253,827 - HMPREF1058_RS14225 0.77 -0.6 1,253,833 - HMPREF1058_RS14225 0.78 -1.0 1,253,846 - HMPREF1058_RS14225 0.79 -1.9 1,253,846 - HMPREF1058_RS14225 0.79 -2.3 1,253,846 - HMPREF1058_RS14225 0.79 +0.2 1,253,952 - HMPREF1058_RS14225 0.90 +0.9 1,253,952 - HMPREF1058_RS14225 0.90 -0.4 1,253,956 + +0.5 1,253,967 - -0.0 1,254,013 - +0.2 1,254,013 - -1.9 1,254,013 - -1.6 1,254,114 - -1.5 1,254,114 - +0.6 1,254,118 + +1.1 1,254,118 + +0.7 1,254,162 + +0.2 1,254,176 - +1.1 1,254,260 - HMPREF1058_RS14220 0.19 +2.5 1,254,301 - HMPREF1058_RS14220 0.25 +2.2 1,254,328 - HMPREF1058_RS14220 0.29 +2.4 1,254,367 - HMPREF1058_RS14220 0.34 +2.9 1,254,488 + HMPREF1058_RS14220 0.52 -0.1 1,254,549 + HMPREF1058_RS14220 0.61 -1.4 1,254,550 - HMPREF1058_RS14220 0.61 +3.6 1,254,584 - HMPREF1058_RS14220 0.66 +1.5 1,254,589 - HMPREF1058_RS14220 0.67 +4.4 1,254,658 + HMPREF1058_RS14220 0.77 -0.9 1,254,673 - +4.2 1,254,750 - +4.3 1,254,816 + -1.2 1,254,860 + +0.7 1,254,941 + +0.2 1,254,941 + +0.8 1,254,988 + +0.4 1,254,997 + -0.7 1,254,997 + +0.4 1,254,999 + -1.0 1,254,999 + -2.5 1,255,092 + HMPREF1058_RS14215 0.13 -0.3 1,255,129 + HMPREF1058_RS14215 0.18 +0.1 1,255,131 + HMPREF1058_RS14215 0.19 +0.1 1,255,133 + HMPREF1058_RS14215 0.19 +0.3 1,255,133 + HMPREF1058_RS14215 0.19 -1.6 1,255,133 + HMPREF1058_RS14215 0.19 +0.1 1,255,208 + HMPREF1058_RS14215 0.30 +0.0 1,255,208 + HMPREF1058_RS14215 0.30 -2.8 1,255,521 + HMPREF1058_RS14215 0.75 -2.4 1,255,694 + -1.6
Or see this region's nucleotide sequence